Rasgrp4 (NM_145149) Mouse Untagged Clone

CAT#: MC220375

Rasgrp4 (untagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4), transcript variant 2, (10ug)



  "NM_145149" in other vectors (4)

CNY 8,650.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220375 representing NM_145149
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGGAAAGACATCAAAAGGAAGTCTCATCAGGAATGCTCTGGGAAGGCAGGAGGGCGGGGTCGGT
CACGTCAGGCCCGCCGCCACAAGACGTGCCCCACCCCCCGGGAAATCAGCAAGGTCATGGCGTCCATGAA
TCTGGGAGTGCTGAGTGAGAGCAGCTGCAGCGAAGATGAGCTATTGGAGGAATGTATCCGCTGCTTTGAC
TCGGCTGGCAGCCTGCGCCGAGGGGACCACATTCTCAAGATGGTGCTCACGATGCACAGCTGGGTGCTGC
CATCCTCAGAGCTCGCTGCCCGTCTGCTGACTTCGTATCAGAAGGCTGCCAAAGATGCCCAAGAGCTAAG
ACAGCTACAGATCTGTTACTTGGTCAGGTACTGGCTGACCCATCACCATGAGGCAGTGCACCAGGAACCC
CAGCTGGAAGCAGTCATAAGCCGGTTTTGGACCACTGTCGCTCAGGAGGGCAACATGGCCCAAAGGAGCC
TGGGAGATGCCTCCAGCCTGCTAAGTCCTGGAGGGCCCGGTCCCCCACCTCCCATGAGCAGCCCAGGCCT
GGGCAAGAAGCGGAAAGTGTCATTGCTATTCGATCACCTGGAGACAGAGGAGCTGGCACAACACCTCACT
TACCTGGAGTTCCGGTCTTTCCAGGCTATCACACCCCAAGACCTGCGGGGCTATGTTTTGCAGGGTTCAG
TGAGAGGTTGTCCAGCTCTGGAAGGTTCTGTGGGTCTGAGCAACAGTGTGTCCCGCTGGGTGCAGGTCAT
GGTGCTGAGTCGTCCTGGGCCTGCACAACGTGCCCAGGTCCTGGACAAATTCATTCGTGTGGCACAGAGG
CTTCACCAGCTGCAGAATTTCAACACACTGATGGCGGTCACGGGGGGTCTGTGTCATAGCGCCATCTCCA
GACTCAAGGACTCCCACGTTCATCTGAGCCCTGACAGCACAAAGGCCCTGCTGGAGCTGACAGAGCTCCT
CTCATCCCACAACAACTATGCCCACTACCGCCGCACCTGGGCCGGCTGTACTGGCTTCCGGCTGCCAGTA
CTGGGTGTGCACCTCAAGGACCTGGTATCTCTATATGAGGCTCATCCAGACAGATTGCCAGATGGCCGCC
TGCACCTACCCAAGCTGAATAGCCTCTATCTACGGCTGCAGGAGCTGATGGCGCTCCAGGGACAGCATCC
TCCCTGCAGTGCCAATGAGGACCTGCTGCACCTGCTGACGCTCTCCCTGGATCTCTTCTACACGGAAGAT
GAGATCTACGAGCTTTCTTATGCCCGGGAACCACGCTGTCCCAAGAGTCTGCCACCGTCCCCCTTCAAAG
CACCAGTGGTGGTAGAGTGGGCCCAGGGTGTGACACCAAAGCCAGACAGCGTGACTCTGGGTCAGCATGT
GGAACAGCTGGTGGAGTCCGTGTTCAAGAACTACGACCCAGAAGGCCGTGGCTCCATCTCTCTGGAGGAC
TTTGAGCGGCTGTCAGGCAACTTCCCGTTCGCCTGCCATGGGCTTCACCCACCTCCCCGCCATGGGAGTG
GCTCCTTCAGCAGAGAGGAGCTGACCAAGTACCTGCTCCATGCCAGTGCCATCTGCTCCAAGCTGGGCCT
GGCCTTCCTGCACGCCTTCCAGGAGGTCACCTTCCGAAAGCCCACGTTCTGTCACAGCTGCAGCGGCTTC
CTCTGGGGGGTCACCAAGCAAGGCTATCGCTGTCGGGACTGTGGGCTGTGTTGTCACAGACACTGCAGGG
ATCAAGTGAGAGTGGAGTGTAAGAAGAGGCCAGAGACTAAGGGTGACCCGGGCCCCCCAGGTGCACCTGT
GCCAGCCACATCACTTCCTCCTGCCAACTGTGGCTCAGAGGAAAGTCTCTCCTATACACTCTCCCCGGAT
CCGGAGTCTGGTTGCCACCTTCGCCATGCTTGGACCCAGACGGAATCCTCACACTCTTCCTGGGAGCCAG
AGGTGGTGCCCTGCCCAGCACGGGTCTTACCATCCAGAGCTTCCTCGAAGCCCAGCGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145149
Insert Size 2022 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145149.4, NP_660131.2
RefSeq Size 4621 bp
RefSeq ORF 2022 bp
Locus ID 233046
UniProt ID Q8BTM9
Gene Summary Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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