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Rfx6 (NM_177306) Mouse Untagged Clone
CAT＃: MC220294
Rfx6 (untagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 2, (10ug)
CNY 8,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4930572O07Rik; Rfxdc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220294 representing NM_177306
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTACAAAACTCACTGCCAGTGCATACTCGACAATGCCATCAATGGGAACTTTGAAGAGATCCAGC ATTTCTTACTACACTTTTGGCAAGGAATGCCAGACCATCTCCTTCCCCTGCTTGAAAATCCTGTTATCAT TGATATTTTCTGTGTCTGTGACTCAATTCTTTATAAGGTTCTTACAGATGTCCTCATTCCTGCAACAATG CAAGAAATGCCTGAAAGTTTGTTAGCAGATATAAGAAATTTTGCTAAAAATTGGGAACAGTGGGTTGTTT CATCCTTGGAAAACTTGCCAGAAGCCCTCATTGATAAGAAAATCCCCATTTTGCGAAGATTTGTATCTTC CCTGAAGCGACAGACATCTTTCTTGCATCTTGCTCAGATTGCCAGACCAGCTCTCTTTGACCAGCATGTG GTGAATGCCATGGTATCTGATATTGAAAAGGTTGACTTAAATAGTATTGGGTCTCAGGCTCTTCTTACCA TATCCAACAGCACAGACACGGAATCTGACATCTACAGTGAACATGACTCTATTACTGTGTTCCAAGAACT GAAAGATCTCCTTAAGAAGAATGCTACAGTGGAGGCATTTATTGAATGGTTGGACACTGTGGTAGAGCAG AGAGTTATTAAGATGAGCAAACAAAATGGAAGATCTCTGAAGAAGAGGGCTCAAGACTTTCTGCTCAAAT GGAGCTTTTTTGGTGCCCGCGTGATGCATAATCTTACCTTGAACAACGCATCAAGTTTTGGCTCTTTCCA TCTGATCCGAATGCTTCTGGATGAGTACATTCTCCTGGCCATGGAGACTCAATTTAACAATGACAAAGAG CAGGAACTACAGAATTTATTGGACAAGTATATGAAGAACTCGGATGCGAGTAAAGCTGCCTTCACAGCTT CCCCGAGCTCTTGCTTTCTGGCCAACCGAAATAAGGCTAGCTCACTTGCCAGTGACACTGTGAAGAACGA AAGCCACGTGGAGACATCCTATGTCCCTCTGCCTTCCAGCCAGCCTGGAGCCATACCCCCTGCTCTGCAC CCATTCTCAACTGAGGACACTGATAACATGCCACTCCCAGGTCAAATAGAGCTTTCACAAAGTACTGGCC ATCTGATGACACCACCGATTTCTCCAGCCATAGCAAGCAGAGGAAGTGTTATTAACCAAGGGCCAATGGC GAGCAGACCCCCGAGCGTGGGCACAGTTCTCTCAGCTCCAACACATTGCTCAACATATGCAGAACCAATT TATCCTACGCTCTCTCCAGCCAACCACGACTTTTATGGGACCAACTCTAACTATCAGACTATGTTTAGGA CACAGTCTCACCCTGCATCAAGCCTCTATGCTCACCGTGCAGAGCATGGGCGGTGCATGGCCTGGACTGA ACAGCAGCTTTCTAGAGACTTCTTTGGTGGCAGTTGTGCTGGGTCTCCATATAATTGTAGGCCACCTTCC AGTTATGGACCATCCACACACACACAAGAGTCACACAGCATGCAGGTTTTGAACACAGGAAGCTTCAATT TCCTTAGTAATGCAGGAGCTGGCAGCTGCCAAGGGTCAACATTGCCTTCTAATTCTCCCAATGGATACTA TGGAAACAATATAAACTACTCAGAGGCACATAGGCTTGGATCGATGGTGAACCAACATGTTTCAGTCATC AGCAGTGTGCGCTCCCTGCCTCCCTACAGTGATATTCATGATCCACTTAACATTTTAGATGACAGCAGCC GGAAGCAGAACAACTCGTTTTATGCAGACACATTGTCTCCTGTTGCATGTCGTACTACTGTAGTAGCTTC CAACTTGCAAACCCAGATTCCTTCATCTTCATCCCAGTGTATGTATGGAACTTCCAATCAGTATCCAGTG CAAGATAGTCTGGACTCCAATGCAGCAAGCAACAGAGAAATGGTGTCCTCTTTACCACCCATCAACACCG TGTTTATGGGGACAGCAGCTGGAGACACTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_177306 |
| Insert Size | 1992 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177306.3, NP_796280.1 |
| RefSeq Size | 3088 bp |
| RefSeq ORF | 1992 bp |
| Locus ID | 320995 |
| UniProt ID | Q8C7R7 |
| Gene Summary | Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224309 | Rfx6 (tGFP-tagged) - Mouse regulatory factor X 6 (Rfx6) transcript variant 2, (10ug) |
CNY 7,320.00 |
|
| MR224309 | Rfx6 (Myc-DDK-tagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 2 |
CNY 6,650.00 |
|
| MR224309L3 | Lenti ORF clone of Rfx6 (Myc-DDK-tagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 2 |
CNY 8,550.00 |
|
| MR224309L4 | Lenti ORF clone of Rfx6 (mGFP-tagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 2 |
CNY 8,550.00 |
