Padi4 (NM_011061) Mouse Untagged Clone

CAT#: MC220244

Padi4 (untagged) - Mouse peptidyl arginine deiminase, type IV (Padi4), (10ug)



  "NM_011061" in other vectors (4)

CNY 4,888.00

CNY 8,550.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Pad4; Pdi4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_011061.2
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAAGGCGCGGTGATCCACGTGGCCCCCGAGCAGCCCACTCACGCCGTGTGTGTGGTGGGCACAG
CGACCCCGCTGGATGTCCGCGGTTCTGCTCCTAAGGGCTACACAACCTTCGGCATCACAGCCTCTCCAGG
AGTCATCGTAGATGTCATCCATGGTCCTCCAGTCAAGAAGAGTACCATGGGGGCCTCCAAATGGCCTCTG
GACCCTGAGCTGGAGGTGACCCTACAGGTGAAAGCAGCCAGCAGCAGAACAGATGATGAAAAGGTTCGAG
TTTCATACTATGGACCCAAGACCTCCCCAGTCCAAGCCCTGATCTACATCACTGGGGTGGAACTGTCCCT
GAGCGCAGATGTCACCCGCACAGGCAGAGTGAAGCCAGCCCAAGCCGGGAAGGATCAGAGCACCTGGACC
TGGGGCCCGGGCGGCCGTGGCGCCATCCTGTTGGTGAACTGTGACAAAGAGGACCCTCAGGCCTCCGGAA
TGGACTTTGAGGATGACAAGATCTTGGACAACAAAGACCTGCAGGACATGTCTCCAATGACCCTAAGCAC
GAAGACGCCCAAAGACTTCTTTGAAAAGTATCAGCTGGTGTTGGAGGTGCCCAAGGCCAAGATGAACAGA
GTGAGAGTCTTCCGGGCCACACGGGGCAAACTGCCGTCCCGGTACAAGGTGGCCCTGGGACCACAACAGT
TCTCGTATTGCCTGGAGCTGCCCGGCGGCCAGCACAGCACAGACTTCTATGTGGAAGGCCTTGCTTTCCC
AGACGCAGACTTCAAAGGGCTCATTCCCCTCACCATCTCCCTGCTGGACAAGTCTAACCCGGAGCTCCCC
GAGGCCCTGGTGTTCCAAGACAGTGTGACGTTCCGTGTGGCCCCCTGGATCATGACCCCCAACACTCAGC
CCCCCCAGGAGGTGTACGTGTGCAGGGTTTCTGACAATGAAGACTTCCTAAAGTCACTAGCTACTCTGAC
CAAGAAAGCCAAGTGCAAGCTGACTGTGTGCCCCGAGGAGGAGAATATAGATGACCAATGGATGCAGGAC
GAAATGGAGATTGGCTACATCCAGGCCCCACACAAGACGCTGCCTGTGGTCTTTGACTCCCCGAGGGACA
GAGGCCTGAAGGATTTCCCTGTCAAGCGAGTTATGGGTCCAAATTTTGGCTATGTGACCCGAAAGCTCTA
TATGTCAGAGCTCACTGGGCTGGATGCCTTTGGGAACCTGGAGGTGAGTCCCCCAGTCACTGTCAGAGGG
AAGGAGTACCCACTGGGCAGAATTCTCATCGGGAATAGCGGTTACTCCAGCAGCGAGAGCCGGGACATGC
ACCAGGCCCTGCAGGACTTCCTGAGCGCCCAGCAGGTGCAGGCCCCCGTGAGGCTCTTCTCCGATTGGCT
CTTTGTGGGTCACGTGGATGAGTTCTTGAGCTTTGTCCCAGCGCGGGACAAGCAGGGTTTTCGGCTGCTG
CTGTCCAGCCCCAGAGCTTGCTATCAGCTGTTCCAGGAGCTACAGAGCCAGGGCCACGGGGAGGCGACAC
TGTTCGAAGGACTCAAGAGGAAAAGGCAGACAATCAATGAAATTCTGTCCAACAAGAAATTAAGAGACCA
GAATGCCTATGTGGAGAGCTGTATCGACTGGAACCGGGCGGTGCTGAAGCGGGAGCTGGGCCTGGCAGAG
GGTGACATCATCGACATTCCGCAGCTCTTCAAGCTCGCGGGGAACTCCAGAGGGAACTCTAAGGCCCAGG
CCTTCTTCCCAAACATGGTGAACATGCTGGTCCTGGGCAAGTACCTGGGCATCCCCAAGCCCTTTGGGCC
CATCATCGATGGCCACTGCTGCCTGGAGGAGGAGGTGCGTTCCCACCTGGAGCCGCTGGGTCTGCACTGC
ACCTTCATCAACGACTTCTACACCTACCACGTGTACAACGGGGAGGTTCACTGTGGCACCAATGTGCGCA
GGAAGCCCTTCACCTTCAAGTGGTGGCACATGGTGCCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_011061
Insert Size 2001 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011061.2, NP_035191.2
RefSeq Size 2640 bp
RefSeq ORF 2001 bp
Locus ID 18602
UniProt ID Q9Z183
Gene Summary Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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