United States
Germany
Japan
United Kingdom
China
Alox15 (NM_009660) Mouse Untagged Clone
CAT＃: MC220233
Alox15 (untagged) - Mouse arachidonate 15-lipoxygenase (Alox15), (10ug)
CNY 7,416.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220233 representing NM_009660
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTCTACCGCATCCGCGTCTCCACCGGGGACTCCGTGTACGCGGGCTCCAACAACGAGGTCTACC TGTGGTTGATCGGACAGCATGGGGAGGCATCTCTCGGGAAGCTGTTCCGACCCTGTCGGAACTCGGAGGC AGAATTCAAGGTGGATGTGTCAGAATACCTTGGGCCACTGCTGTTTGTGAGAGTGCAGAAATGGCATTAT CTCAAAGAGGACGCCTGGTTCTGCAACTGGATTTCTGTGAAGGGCCCCGGAGACCAGGGATCGGAGTACA CGTTCCCCTGTTACCGATGGGTTCAGGGCACCAGCATCCTGAACCTCCCTGAGGGCACTGGCTGCACCGT GGTTGAAGACTCTCAAGGCCTGTTCAGGAACCACAGGGAGGAGGAACTGGAAGAAAGGAGGAGTCTGTAC AGGTGGGGCAACTGGAAGGATGGCACAATCCTGAACGTGGCGGCGACCAGTATCTCTGACCTCCCTGTAG ACCAGCGATTTCGAGAGGACAAAAGACTTGAATTTGAAGCTTCACAGGTTCTGGGGACAATGGACACCGT TATTAACTTCCCTAAAAACACTGTGACCTGCTGGAAAAGCCTAGATGACTTCAACTATGTTTTCAAGAGT GGCCACACCAAGATGGCTGAGCGGGTTCGAAACTCCTGGAAAGAGGATGCTTTCTTTGGGTACCAGTTTC TCAATGGTGCTAACCCCATGGTGCTGAAGCGGTCTACTTGTCTCCCTGCCCGCCTGGTATTCCCTCCGGG GATGGAGAAGCTACAGGCCCAGCTGGATGAGGAGCTCAAGAAAGGCACTCTGTTTGAAGCGGATTTCTTC CTTCTGGATGGGATCAAGGCCAATGTCATCCTTTGTAGTCAGCAGTACCTGGCTGCCCCCCTCGTCATGC TGAAGCTGCAGCCCGATGGGCAACTCTTGCCTATAGCCATCCAGCTCGAACTGCCCAAAACTGGGTCTAC CCCACCGCCGATTTTCACGCCCTTGGATCCCCCAATGGACTGGCTCCTGGCCAAATGCTGGGTCCGAAGC TCAGACTTACAGCTTCATGAGCTACAGGCTCATCTTCTGAGGGGACACTTGGTGGCTGAGGTCTTTGCTG TGGCCACCATGAGGTGCCTGCCTTCCGTGCACCCTGTTTTTAAGCTTCTAGTTCCTCACCTACTCTACAC CATGGAAATCAATGTCCGGGCCAGGAGCGACCTGATCTCAGAGAGAGGCTTTTTTGACAAGGTGATGAGC ACAGGTGGAGGAGGCCACCTGGATCTTCTCAAGCAAGCTGGAGCCTTCCTGACCTACAGCTCATTGTGTC CCCCTGATGACTTGGCTGAGCGAGGACTCCTGGATATTGACACTTGCTTCTATGCTAAAGATGCCCTGCA GCTCTGGCAAGTCATGAATCGGTACGTGGTGGGAATGTTCGATCTCTACTACAAGACCGACCAAGCTGTT CAGGATGACTATGAACTGCAGAGCTGGTGTCAAGAGATCACTGAGATTGGGTTGCAAGGTGCCCAGGACA GAGGCTTCCCCACCTCCCTCCAGTCCCGGGCTCAGGCTTGCCACTTCATCACCATGTGCATCTTCACATG CACTGCGCAGCACTCTTCCATCCATCTTGGCCAGCTGGATTGGTTCTACTGGGTTCCTAATGCACCCTGC ACCATGCGGCTGCCACCACCTAAAACCAAGGACGCGACGATGGAGAAGCTGATGGCGACGCTGCCCAATC CTAATCAGTCTACTCTCCAGATAAATGTCGTTTGGCTCCTGGGCAGACGCCAGGCTGTTATGGTGCCCCT GGGCCAGCATTCAGAGGAACACTTCCCAAACCCTGAGGCCAAAGCTGTGCTGAAGAAGTTCAGAGAGGAG CTGGCTGCCTTGGATAAGGAAATTGAGATTCGTAACAAGAGCTTGGACATACCTTATGAGTACCTGCGGC CCAGCCTGGTAGAAAACAGCGTGGCCATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009660 |
| Insert Size | 1992 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009660.3, NP_033790.3 |
| RefSeq Size | 2414 bp |
| RefSeq ORF | 1992 bp |
| Locus ID | 11687 |
| UniProt ID | P39654 |
| Gene Summary | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226258 | Alox15 (tGFP-tagged) - Mouse arachidonate 15-lipoxygenase (Alox15), (10ug) |
CNY 9,000.00 |
|
| MR226258 | Alox15 (Myc-DDK-tagged) - Mouse arachidonate 15-lipoxygenase (Alox15) |
CNY 7,400.00 |
|
| MR226258L3 | Lenti ORF clone of Alox15 (Myc-DDK-tagged) - Mouse arachidonate 15-lipoxygenase (Alox15) |
CNY 8,550.00 |
|
| MR226258L4 | Lenti ORF clone of Alox15 (mGFP-tagged) - Mouse arachidonate 15-lipoxygenase (Alox15) |
CNY 8,550.00 |
