Picalm (NM_146194) Mouse Untagged Clone

CAT#: MC220207

Picalm (untagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm), (10ug)



  "NM_146194" in other vectors (4)

CNY 7,384.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CALM; CLTH; fit-1; fit1; mKIAA4114; PAP180
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220207 representing NM_146194
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGCCAGAGCCTGACGGACCGAATCACCGCGGCCCAGCACAGTGTCACTGGCTCCGCGGTATCTA
AGACAGTATGCAAGGCCACGACCCACGAGATCATGGGCCCCAAGAAAAAGCACCTGGACTACTTAATTCA
GTGTACAAATGAGATGAATGTGAACATCCCACAGTTGGCGGACAGTTTGTTTGAAAGAACTACTAACAGT
AGTTGGGTGGTGGTCTTCAAATCACTCATTACAACTCATCATTTGATGGTGTATGGAAATGAGCGTTTCA
TTCAGTATTTGGCTTCAAGAAACACATTGTTTAACTTAAGCAATTTTTTGGATAAAAGTGGATTGCAAGG
ATATGATATGTCTACATTTATTAGACGGTATAGTAGATACTTAAATGAAAAAGCAGTTTCATACAGACAA
GTTGCATTCGATTTCACAAAAGTGAAGAGAGGAGCTGATGGAGTTATGAGAACAATGAACACAGAAAAAC
TCTTAAAAACTGTACCAATTATCCAAAATCAAATGGATGCACTTCTTGATTTTAATGTTAATAGTAATGA
ACTTACAAATGGGGTAATAAATGCTGCCTTCATGCTCCTCTTCAAAGATGCCATTAGACTATTTGCAGCA
TACAATGAAGGAATTATTAATTTATTGGAAAAATATTTTGATATGAAAAAGAACCAGTGCAAAGAAGGTC
TTGACATCTATAAGAAGTTTCTGACTAGGATGACAAGAATCTCAGAGTTTCTGAAAGTTGCAGAGCAAGT
TGGAATTGACAGAGGAGATATTCCAGATCTTTCACAGGCCCCCAGCAGTCTTCTTGATGCTTTAGAACAA
CATTTAGCTTCCTTGGAAGGAAAGAAAATCAAAGATTCCACAGCTGCAAGCAGGGCTACAACACTTTCCA
ATGCAGTCTCTTCTTTGGCAAGCACTGGCCTATCTCTGACCAAAGTGGATGAAAGGGAAAAACAGGCAGC
ATTAGAGGAAGAACAGGCTCGTTTAAAAGCATTAAAGGAACAGCGTCTAAAAGAACTTGCAAAGAAACCG
CATACCTCTCTAACAACTGCAGCCTCTCCGGTGTCCACCTCAGCAGGGGGAATAATGACTGCACCAGCCA
TTGACATATTTTCTACCCCTAGTTCTTCTAACAGCACATCTAAGCTGCCAAATGACCTGCTTGACTTGCA
GCAGCCAACCTTTCACCCGTCTGTCCACGCCATGTCGGCTGCTCCTCAGGGAGCGAGTACATGGGGAGAT
CCTTTCTCTGCTACTCTAGATGCTGTTGAGGATGCCATTCCAAGCTTAAACCCTTTCCTCACAAAAAGTA
GTGGTGATGTTCACCTGCCCATTGCTTCAGATGTATCCACTTTTACTACTAGGACACCTACTCATGAGAT
GTTTGTTGGATTCAGTCCTTCTCCAGTTGCACAGCCACATTCTTCAGCTGGCCTTAATGTTGACTTTGAA
TCTGTGTTTGGAAATAAATCTACGAATGTTGCTGTAGATTCTGGTGGCTTTGATGAACTAGGTGGACTTC
TCAAACCAACAGTGGCCTCCCAGAACCAGAGTCTTCCTGTTGCCAAACTTCCGCCTAACAAATTAGTGTC
TGACGACTTGGATTCATCTTTAGCCAACCTTGTGGGCAATCTTGGCATTGGAAATGGAACCACTAAGAAT
GATGTAAGTTGGAGTCAACCAGGTGAAAAGAAGTTAACTGGAGGATCTAACTGGCAACCAAAGGTTGCAC
CAACAACTGCCTGGAGCGCTGCAACAATGAATGGCATGCATTTTCCACAATACGCGCCCCCTGTAATGGC
CTATCCTGCTACTACACCAACGGGCATGATAGGATATGGAATTCCTCCTCAGATGGGAAGTGTACCTGTA
ATGACACAACCAACCTTAATATACAGCCAGCCTGTCATGAGACCACCAAACCCCTTTGGCCCTGTATCAG
GAGCACAGATACAGTTTATGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_146194
Insert Size 1983 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_146194.4, NP_666306.2
RefSeq Size 4174 bp
RefSeq ORF 1983 bp
Locus ID 233489
UniProt ID Q7M6Y3
Gene Summary Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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