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Picalm (NM_146194) Mouse Untagged Clone
CAT＃: MC220207
Picalm (untagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm), (10ug)
CNY 7,384.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CALM; CLTH; fit-1; fit1; mKIAA4114; PAP180 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220207 representing NM_146194
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCCAGAGCCTGACGGACCGAATCACCGCGGCCCAGCACAGTGTCACTGGCTCCGCGGTATCTA AGACAGTATGCAAGGCCACGACCCACGAGATCATGGGCCCCAAGAAAAAGCACCTGGACTACTTAATTCA GTGTACAAATGAGATGAATGTGAACATCCCACAGTTGGCGGACAGTTTGTTTGAAAGAACTACTAACAGT AGTTGGGTGGTGGTCTTCAAATCACTCATTACAACTCATCATTTGATGGTGTATGGAAATGAGCGTTTCA TTCAGTATTTGGCTTCAAGAAACACATTGTTTAACTTAAGCAATTTTTTGGATAAAAGTGGATTGCAAGG ATATGATATGTCTACATTTATTAGACGGTATAGTAGATACTTAAATGAAAAAGCAGTTTCATACAGACAA GTTGCATTCGATTTCACAAAAGTGAAGAGAGGAGCTGATGGAGTTATGAGAACAATGAACACAGAAAAAC TCTTAAAAACTGTACCAATTATCCAAAATCAAATGGATGCACTTCTTGATTTTAATGTTAATAGTAATGA ACTTACAAATGGGGTAATAAATGCTGCCTTCATGCTCCTCTTCAAAGATGCCATTAGACTATTTGCAGCA TACAATGAAGGAATTATTAATTTATTGGAAAAATATTTTGATATGAAAAAGAACCAGTGCAAAGAAGGTC TTGACATCTATAAGAAGTTTCTGACTAGGATGACAAGAATCTCAGAGTTTCTGAAAGTTGCAGAGCAAGT TGGAATTGACAGAGGAGATATTCCAGATCTTTCACAGGCCCCCAGCAGTCTTCTTGATGCTTTAGAACAA CATTTAGCTTCCTTGGAAGGAAAGAAAATCAAAGATTCCACAGCTGCAAGCAGGGCTACAACACTTTCCA ATGCAGTCTCTTCTTTGGCAAGCACTGGCCTATCTCTGACCAAAGTGGATGAAAGGGAAAAACAGGCAGC ATTAGAGGAAGAACAGGCTCGTTTAAAAGCATTAAAGGAACAGCGTCTAAAAGAACTTGCAAAGAAACCG CATACCTCTCTAACAACTGCAGCCTCTCCGGTGTCCACCTCAGCAGGGGGAATAATGACTGCACCAGCCA TTGACATATTTTCTACCCCTAGTTCTTCTAACAGCACATCTAAGCTGCCAAATGACCTGCTTGACTTGCA GCAGCCAACCTTTCACCCGTCTGTCCACGCCATGTCGGCTGCTCCTCAGGGAGCGAGTACATGGGGAGAT CCTTTCTCTGCTACTCTAGATGCTGTTGAGGATGCCATTCCAAGCTTAAACCCTTTCCTCACAAAAAGTA GTGGTGATGTTCACCTGCCCATTGCTTCAGATGTATCCACTTTTACTACTAGGACACCTACTCATGAGAT GTTTGTTGGATTCAGTCCTTCTCCAGTTGCACAGCCACATTCTTCAGCTGGCCTTAATGTTGACTTTGAA TCTGTGTTTGGAAATAAATCTACGAATGTTGCTGTAGATTCTGGTGGCTTTGATGAACTAGGTGGACTTC TCAAACCAACAGTGGCCTCCCAGAACCAGAGTCTTCCTGTTGCCAAACTTCCGCCTAACAAATTAGTGTC TGACGACTTGGATTCATCTTTAGCCAACCTTGTGGGCAATCTTGGCATTGGAAATGGAACCACTAAGAAT GATGTAAGTTGGAGTCAACCAGGTGAAAAGAAGTTAACTGGAGGATCTAACTGGCAACCAAAGGTTGCAC CAACAACTGCCTGGAGCGCTGCAACAATGAATGGCATGCATTTTCCACAATACGCGCCCCCTGTAATGGC CTATCCTGCTACTACACCAACGGGCATGATAGGATATGGAATTCCTCCTCAGATGGGAAGTGTACCTGTA ATGACACAACCAACCTTAATATACAGCCAGCCTGTCATGAGACCACCAAACCCCTTTGGCCCTGTATCAG GAGCACAGATACAGTTTATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_146194 |
| Insert Size | 1983 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_146194.4, NP_666306.2 |
| RefSeq Size | 4174 bp |
| RefSeq ORF | 1983 bp |
| Locus ID | 233489 |
| UniProt ID | Q7M6Y3 |
| Gene Summary | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224816 | Picalm (tGFP-tagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm), (10ug) |
CNY 7,220.00 |
|
| MR224816 | Picalm (Myc-DDK-tagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm) |
CNY 6,560.00 |
|
| MR224816L3 | Lenti ORF clone of Picalm (Myc-DDK-tagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm) |
CNY 8,460.00 |
|
| MR224816L4 | Lenti ORF clone of Picalm (mGFP-tagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm) |
CNY 8,460.00 |
