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Foxk2 (NM_001080932) Mouse Untagged Clone
CAT＃: MC220094
Foxk2 (untagged) - Mouse forkhead box K2 (Foxk2), (10ug)
CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110054H05Rik; 5730434B08Rik; 6230415M23Rik; ILF; Ilf1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220094 representing NM_001080932
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCGGCGGCGCTCTCGGGCGCGGGCGCCCCTCCCGCGGGCGGCGGGGCCGGGGGCGGCGGAT CCCCGCCGGGCGGCTGGGCCGTGGCGCGGCTCGAAGGCCGCGAGTTCGAGTACCTCATGAAGAAGCGGTC GGTGACCATCGGCCGCAACTCATCGCAGGGCTCGGTAGATGTGAGCATGGGCCACTCGAGCTTCATCTCG CGGCGCCACCTGGAGATCTTCACGCCCCCGGGCGGCGGCCATAGCGCGGCCGCCCCGGAACCCGCGCAGC CCCGGCCGGACGCGGGCGGCGACTTCTACCTGCGCTGTCTCGGCAAGAACGGCGTGTTCGTGGACGGGGT GTTCCAGCGGCGGGGGGCGCCGCCGCTGCAGCTGCCGCGCGTGTGCACTTTCAGGTTTCCGAGCACAAAT ATCAAGATAACATTCACAGCCCTATCCAGCGAGAAGAGAGAGAAGCAGGAAGCACCTGAGTCGCCAGTGA AGCCTGTGCAGCCACACATCTCACCCCTGACCATCAACATTCCAGACACGATGGCCCACCTCATCAGCCC CCTTCCTTCCCCCACGGGAACTATCAGTGCTGCCAACTCCTGCCCATCCAGTCCTCGCGGAGCAGGGTCT TCAGGGTACAAAGTGGGCCGTGTGATGCCATCGGACCTCAGTTTAATGGCTGACAACTCCCAGCCAGAAA ATGAAAAGGAAGCGTCAGGTGGAGACAGCCCAAAGGATGACTCAAAGCCACCTTACTCCTATGCACAGTT GATAGTACAAGCAATTACAATGGCTCCTGATAAACAACTTACCCTAAATGGGATTTATACACACATCACT AAAAACTATCCATACTACAGGACTGCGGACAAGGGCTGGCAGAATTCAATTCGCCACAATCTCTCTCTGA ACCGTTATTTCATCAAAGTGCCACGTTCCCAGGAAGAACCAGGCAAAGGCTCCTTCTGGAGGATAGACCC TGCCTCCGAGAGCAAGCTGGTAGAGCAGGCTTTTAGGAAAAGACGGCCTAGGGGCGTGCCTTGCTTTAGA ACCCCCCTGGGACCTCTCTCCTCTAGGAGTGCCCCAGCTTCTCCCAACCATGCCGGAGTGCTGTCTGCTC ACTCCAGTGGCGCCCAGACCCCCGAGAGCCTGTCGAGGGAGGGCTCCCCAGCCCCCTTGGAGCCTGAGCC TGGCGCCTCACAGCCCAAGCTTGCCGTCATCCAGGAGGCCAGGTTTGCCCAGAGTGCACCAGGGTCACCT CTCTCTAGTCAGCCCGTCTTAATTACTGTCCAGCGGCAGCTACCCCCGGCCATTAAGCCTGTCACCTACA CTGTTGCAACCCCAGTGACCACACCCACCTCTCAGCCACCTGTTGTGCAGACGGTCCATGTTGTTCATCA GATACCAGCAGTGTCAGTCACCAGTGTGGCTGGACTGGCCCCAGCAAACACATACACAGTTGCTGGACAA GCTGTGGTCACTCAGGCTGCGGTGCTGGCTCCTCCTAACCCTGAACCACAAGAGAATGGCGACCACAGAG AAGTCAGAGTGAAAGTAGAACCTGTCCCTGCAATTAGCCCAGCTACACTAGGCGCTGCTAGCCGAATCAT CCAGACATCACAGGGCACCCCTGTTCAGACAGTGACCATAGTGCAGCAGGCGCCTTTAGGTCAACACCAG CTTCCAATAAAAACTGTAACGCAAAACGGAGCTCATGTGGTACCCATGCCTACAGCAGTGCACAGCCAGG TGAACAATGCAGCAGCAAGTCCTCTCCATATGTTGGCCACCCATGCATCAGCATCGGCATCCCTGCCCAC AAAGCGCCAGAACGGTGACCAGGCAGAGCAGCCAGAGCTCAAGCGAGTTAAAGCAGAAGATGGCGAGAGC ATTGTCATTGCCCTGAGTGTGGATGCACCACCAGCAGCTGTGAGAGAAAAGGCAATCCAGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001080932 |
| Insert Size | 1956 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080932.2, NP_001074401.2 |
| RefSeq Size | 5032 bp |
| RefSeq ORF | 1956 bp |
| Locus ID | 68837 |
| UniProt ID | Q3UCQ1 |
| Gene Summary | Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (PubMed:25402684, PubMed:29861159, PubMed:30700909). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:25402684, PubMed:29861159, PubMed:30700909). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (PubMed:30700909). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (PubMed:30700909). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (PubMed:30700909). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (PubMed:25402684). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (By similarity). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRT[G/T]NB-3' sequence (By similarity). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (By similarity). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216773 | Foxk2 (tGFP-tagged) - Mouse forkhead box K2 (Foxk2), (10ug) |
CNY 6,270.00 |
|
| MR216773 | Foxk2 (Myc-DDK-tagged) - Mouse forkhead box K2 (Foxk2) |
CNY 4,848.00 |
|
| MR216773L3 | Lenti ORF clone of Foxk2 (Myc-DDK-tagged) - Mouse forkhead box K2 (Foxk2) |
CNY 7,600.00 |
|
| MR216773L4 | Lenti ORF clone of Foxk2 (mGFP-tagged) - Mouse forkhead box K2 (Foxk2) |
CNY 7,600.00 |
