Tcf3 (NM_001164147) Mouse Untagged Clone
CAT#: MC220077
Tcf3 (untagged) - Mouse transcription factor 3 (Tcf3), transcript variant 1, (10ug)
CNY 8,360.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | A1; AA408400; ALF2; AW209082; bHLHb21; E2A; E12; E12/E47; E47; KA1; ME2; Pan1; Pan2; TCF-3; Tcfe2a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC220077 representing NM_001164147
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAACCAGTCTCAGAGAATGGCACCCGTGGGCTCTGACAAGGAACTGAGTGACCTCCTGGACTTCA GCATGATGTTCCCGCTACCTGTGGCCAATGGGAAGAGCCGGCCCGCCTCCCTCGGGGGAACCCAGTTTGC AGGCTCAGGACTGGAGGACCGACCCAGCTCAGGCTCCTGGGGCAGCAGTGACCAGAACAGTTCTTCCTTT GACCCTAGCCGGACATACAGCGAAGGTGCCCACTTCAGTGACTCCCACAGCAGCCTGCCGCCTTCCACGT TCCTAGGAGCTGGGCTTGGAGGCAAGGGCAGTGAGCGGAATGCCTATGCCACCTTTGGGAGAGACACCAG TGTTGGCACCTTGAGTCAGGCTGGCTTCCTGCCAGGTGAGCTGAGCCTCAGCAGTCCCGGGCCACTGTCC CCATCGGGCATCAAGAGCAGCTCCCAGTATTACCCCTCATTCCCCAGCAACCCTCGTCGGAGAGCTGCAG ATGGTGGCCTGGCAGATACTCAGCCGAAGAAGGTCCGGAAGGTTCCGCCTGGTCTCCCTTCCTCGGTGTA TCCGCCCAGCTCAGGTGACAGCTACAGCAGGGATGCTGCAGCCTACCCCTCCGCCAAGACCCCCAGCAGC GCTTACCCCTCCCCCTTCTACGTGGCAGATGGCAGCCTGCACCCATCAGCTGAGCTCTGGAGTACGCCTA GCCAGGTGGGCTTTGGGCCCATGCTAGGTGACGGCTCTTCCCCTCTGCCCCTTGCACCGGGCAGCAGCTC CGTGGGCAGTGGTACCTTTGGGGGCCTCCAGCAGCAGGATCGCATGGGCTACCAGCTGCATGGATCTGAG GTTAATGGCTCGCTCCCAGCTGTATCCAGCTTTTCGGCTGCCCCTGGCACTTACAGTGGGACTTCCGGCC ACACGCCCCCTGTGAGTGGGGCCGCAGCTGAAAGCCTCCTAGGCACCCGAGGGACTACAGCCAGCAGCTC AGGGGATGCCCTTGGGAAGGCACTGGCCTCGATCTACTCCCCGGATCACTCCAGCAATAATTTCTCACCT AGCCCCTCAACGCCTGTGGGTTCACCCCAGGGCCTGCCAGGGACATCACAGTGGCCCCGGGCAGGAGCGC CCAGTGCCTTATCCCCCAACTACGATGCAGGTCTCCATGGCCTGCAGAGCAAGATGGAGGACCGCTTGGA CGAGGCCATCCATGTCCTGCGAAGCCACGCTGTTGGCACCGCTAGCGATCTCCATGGGCTTTTGCCTGGC CATGGCGCACTGACCACGAGCTTCACCGGCCCCATGTCACTGGGCGGGCGGCATGCCGGCCTGGTCGGGG GAAGCCATCCTGAGGAGGGCCTCACAAGTGGGGCCAGTCTTTTGCATAACCATGCCAGCCTCCCCAGCCA GCCCAGTTCCCTCCCTGACCTCTCACAGAGACCTCCCGACTCCTATAGTGGACTCGGGAGGGCAGGCACA ACAGCGGGTGCCAGCGAGATCAAGCGGGAGGAGAAAGAGGATGAGGAAATCGCATCAGTAGCCGACGCCG AAGAGGACAAGAAGGACCTGAAGGTCCCACGCACGCGCACCAGCCCAGACGAGGACGAGGACGACCTTCT CCCCCCAGAGCAGAAGGCGGAGCGGGAGAAGGAGCGCCGGGTGGCCAATAATGCCCGAGAGCGCCTGCGG GTCCGCGACATCAATGAGGCCTTTAAGGAGCTCGGCCGCATGTGCCAGCTGCACCTCAGCAGCGAGAAGC CGCAGACCAAACTGCTCATCCTGCACCAGGCCGTGGCCGTCATCCTCAGCCTGGAGCAGCAGGTGCGAGA ACGCAACCTGAACCCCAAAGCAGCCTGCTTGAAGCGGAGGGAGGAGGAGAAGGTGTCTGGCGTGGTCGGG GACCCACAGCTGGCCCTGTCAGCCGCCCACCCGGGCCTGGGTGAGGCCCACAACCCAGCCGGGCACCTGT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001164147 |
Insert Size | 1962 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164147.1, NP_001157619.1 |
RefSeq Size | 3320 bp |
RefSeq ORF | 1962 bp |
Locus ID | 21423 |
Gene Summary | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG225776 | Tcf3 (tGFP-tagged) - Mouse transcription factor 3 (Tcf3) transcript variant 1, (10ug) |
CNY 8,888.00 |
|
MR225776 | Tcf3 (Myc-DDK-tagged) - Mouse transcription factor 3 (Tcf3), transcript variant 1 |
CNY 7,288.00 |
|
MR225776L3 | Lenti ORF clone of Tcf3 (Myc-DDK-tagged) - Mouse transcription factor 3 (Tcf3), transcript variant 1 |
CNY 8,460.00 |
|
MR225776L4 | Lenti ORF clone of Tcf3 (mGFP-tagged) - Mouse transcription factor 3 (Tcf3), transcript variant 1 |
CNY 8,460.00 |