United States
Germany
Japan
United Kingdom
China
Trp73 (NM_011642) Mouse Untagged Clone
CAT＃: MC219984
Trp73 (untagged) - Mouse transformation related protein 73 (Trp73), transcript variant 1, (10ug)
CNY 7,136.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | delta; deltaNp73; p7; p73; TAp; TAp73; Tp73 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_011642.3
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCAGCGTTGGGGAGATGGCCCAGACCTCTTCTTCCTCCTCCTCCACCTTCGAGCACCTGTGGA GTTCTCTAGAGCCAGACAGCACCTACTTTGACCTCCCCCAGCCCAGCCAAGGGACTAGCGAGGCATCAGG CAGCGAGGAGTCCAACATGGATGTCTTCCACCTGCAAGGCATGGCCCAGTTCAATTTGCTCAGCAGTGCC ATGGACCAGATGGGCAGCCGTGCGGCCCCGGCGAGCCCCTACACCCCGGAGCACGCCGCCAGCGCGCCCA CCCACTCGCCCTACGCGCAGCCCAGCTCCACCTTCGACACCATGTCTCCGGCGCCTGTCATCCCTTCCAA TACCGACTACCCCGGCCCCCACCACTTCGAGGTCACCTTCCAGCAGTCGAGCACTGCCAAGTCGGCCACC TGGACATACTCCCCACTCTTGAAGAAGTTGTACTGTCAGATTGCTAAGACATGCCCCATCCAGATCAAAG TGTCCACACCACCACCCCCGGGCACGGCCATCCGGGCCATGCCTGTCTACAAGAAGGCAGAGCATGTGAC CGACATTGTTAAGCGCTGCCCCAACCACGAGCTTGGAAGGGACTTCAATGAAGGACAGTCTGCCCCGGCT AGCCACCTCATCCGTGTAGAAGGCAACAACCTCGCCCAGTACGTGGATGACCCTGTCACCGGAAGGCAGA GTGTGGTTGTGCCGTATGAACCCCCACAGGTGGGAACAGAATTTACCACCATCCTGTACAACTTCATGTG TAACAGCAGCTGTGTGGGGGGCATGAATCGGAGGCCCATCCTTGTCATCATCACCCTGGAGACCCGGGAT GGACAGGTCCTGGGCCGCCGGTCTTTCGAGGGTCGCATCTGTGCCTGTCCTGGCCGTGACCGCAAAGCTG ATGAAGACCATTACCGGGAGCAACAGGCTCTGAATGAAAGTACCACCAAAAATGGAGCTGCCAGCAAACG TGCATTCAAGCAGAGCCCCCCTGCCATCCCTGCCCTGGGTACCAACGTGAAGAAGAGACGCCACGGGGAC GAGGACATGTTCTACATGCACGTGCGAGGCCGGGAGAACTTTGAGATCTTGATGAAAGTCAAGGAGAGCC TAGAACTGATGGAGCTTGTGCCCCAGCCTTTGGTTGACTCCTATCGACAGCAGCAGCAGCAGCAGCTCCT ACAGAGGCCGAGTCACCTGCAGCCTCCATCCTATGGGCCCGTGCTCTCCCCAATGAACAAGGTACACGGT GGTGTCAACAAACTGCCCTCCGTCAACCAGCTGGTGGGCCAGCCTCCCCCGCACAGCTCAGCAGCTGGGC CCAACCTGGGGCCCATGGGCTCCGGGATGCTCAACAGCCACGGCCACAGCATGCCGGCCAATGGTGAGAT GAATGGAGGCCACAGCTCCCAGACCATGGTTTCGGGATCCCACTGCACCCCGCCACCCCCCTATCATGCA GACCCCAGCCTCGTCAGTTTTTTGACAGGGTTGGGGTGTCCAAACTGCATCGAGTGCTTCACTTCCCAAG GGTTGCAGAGCATCTACCACCTGCAGAACCTTACCATCGAGGACCTTGGGGCTCTGAAGGTCCCTGACCA GTACCGTATGACCATCTGGAGGGGCCTACAGGACCTGAAGCAGAGCCATGACTGCGGCCAGCAACTGCTA CGCTCCAGCAGCAACGCGGCCACCATCTCCATCGGCGGCTCTGGCGAGCTGCAGCGGCAGCGGGTCATGG AAGCCGTGCATTTCCGTGTGCGCCACACCATCACGATCCCCAACCGTGGAGGCGCAGGTGCGGTGACAGG TCCCGACGAGTGGGCGGACTTTGGCTTTGACCTGCCTGACTGCAAGTCCCGTAAGCAGCCCATCAAAGAG GAGTTCACAGAGACAGAGAGCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011642 |
| Insert Size | 1917 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011642.3, NP_035772.2 |
| RefSeq Size | 5046 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 22062 |
| UniProt ID | Q9JJP2 |
| Gene Summary | This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes isoform a, also known as TAp73alpha. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Transcription factor TAp73 and microRNA-449 complement each other to support multiciliogenesis
,null,
Cell Death and Differentiation
,PubMed ID 31068677
[Trp73]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227042 | Trp73 (tGFP-tagged) - Mouse transformation related protein 73 (Trp73) transcript variant 1, (10ug) |
CNY 9,200.00 |
|
| MR227042 | Trp73 (Myc-DDK-tagged) - Mouse transformation related protein 73 (Trp73), transcript variant 1 |
CNY 6,370.00 |
|
| MR227042L3 | Lenti ORF clone of Trp73 (Myc-DDK-tagged) - Mouse transformation related protein 73 (Trp73), transcript variant 1 |
CNY 8,270.00 |
|
| MR227042L4 | Lenti ORF clone of Trp73 (mGFP-tagged) - Mouse transformation related protein 73 (Trp73), transcript variant 1 |
CNY 8,270.00 |
