Scnn1b (NM_011325) Mouse Untagged Clone
CAT#: MC219982
Scnn1b (untagged) - Mouse sodium channel, nonvoltage-gated 1 beta (Scnn1b), (10ug)
CNY 7,136.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219982 representing NM_011325
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTGAAGAAGTACCTCCTGAAGTGCCTGCACCGGCTGCAGAAGGGCCCAGGCTACACCTACAAGG AGCTGCTAGTGTGGTACTGCAATAACACCAACACCCACGGCCCCAAACGCATCATCTGTGAGGGGCCCAA GAAGAAGGCCATGTGGTTCCTGCTTACGCTGCTCTTCGCCTGCCTGGTGTGCTGGCAGTGGGGAGTCTTC ATCCAGACCTACCTGAGCTGGGAGGTCAGCGTCTCGCTCTCCATGGGCTTCAAGACGATGAACTTCCCGG CGGTCACCGTCTGCAATTCCAGCCCCTTCCAGTACTCCAAGGTCAAGCACTTGCTCAAGGACTTGGATGA GCTAATGGAGGCAGTCCTGGAAAAGATTCTGGCTCCAGAGGCCAGCCACAGCAACACCACCAGGACCCTG AACTTTACCATCTGGAACCACACACCCCTGGTCCTTATTGATGAGCGGAACCCTGACCACCCGGTGGTTC TCAATTTGTTTGGGGACAGCCACAACAGCAGCAACCCGGCTCCAGGAAGCACCTGCAATGCCCAGGGATG CAAAGTGGCCATGAGGCTGTGCAGTGCCAATGGAACTGTGTGTACCTTGCGGAACTTCACCAGTGCCACC CAGGCCGTAACTGAGTGGTACATCCTGCAGGCCACCAACATCTTCTCACAAGTGCTTCCTCAGGACCTGG TGGGGATGGGCTATGCCCCTGATCGCATAATCCTAGCTTGCCTGTTTGGAACAGAGCCCTGCAGTCATCG GAACTTCACGCCTATCTTCTACCCTGATTATGGCAACTGCTACATCTTCAACTGGGGCATGACAGAGGAG ACACTTCCTTCTGCCAACCCTGGGACTGAATTTGGTCTCAAGTTGATCCTGGACATCGGTCAGGAGGACT ATGTCCCCTTCCTTGCGTCCACAGCAGGGGCTAGGCTGATGCTTCATGAGCAGAGGACGTACCCCTTCAT TAGAGAGGAGGGCATCTATGCCATGGCAGGAACTGAGACGTCTATTGGGGTGCTGGTGGACAAGCTGCAA CGCAAGGGGGAGCCCTACAGTCCCTGCACCATGAACGGCTCCGATGTTGCCATAAAGAACCTCTACAGTG TCTACAACACCACCTATTCCATCCAGGCCTGTCTTCATTCCTGTTTCCAAGACCACATGATCCGTAACTG CAGTTGTGGCCACTACTTATACCCACTGCCTGAAGGAGAGAAATACTGCAACAACAGGGACTTCCCAGAC TGGGCCTATTGCTATCTAAACCTGCAGATGAGTGTGACCCAGAGAGAGACCTGCCTCAGCATGTGCAAGG AGTCCTGCAATGACACCCAGTATAAGATGACCATCTCCATGGCTGACTGGCCATCTGAGGCCTCTGAGGA TTGGATCTTACATGTCCTGTCTCAGGAGCGGGACCAGAGCTCGAATATCACCCTGAGCAGGAAGGGTATT GTCAAGCTCAATATCTACTTCCAAGAGTTCAACTACCGTACCATTGAGGAATCTCCAGCCAACAATATTG TGTGGCTGCTCTCTAACCTGGGGGGCCAGTTTGGCTTCTGGATGGGGGGCTCGGTGCTGTGCCTCATTGA GTTTGGGGAGATCATTATCGACTTCATTTGGATCACCATCATCAAGCTGGTGGCCTCCTGCAAAGGCCTG CGCAGGAGGCGGCCACAGGCACCCTACACTGGCCCACCGCCCACTGTGGCTGAGCTGGTAGAGGCCCACA CCAACTTTGGCTTCCAGCCTGACACAACCAGCTGCAGGCCCCACGGCGAGGTCTACCCTGACCAACAGAC TCTGCCCATCCCGGGGACTCCACCCCCCAACTATGACTCCCTGAGGCTGCAGCCGCTGGACACCATGGAG TCGGACAGTGAGGTGGAGGCCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_011325 |
Insert Size | 1917 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_011325.2, NP_035455.1 |
RefSeq Size | 2694 bp |
RefSeq ORF | 1917 bp |
Locus ID | 20277 |
UniProt ID | Q9WU38 |
Gene Summary | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shortest transcript. Both variants 1 and 2 encode the same protein. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG227329 | Scnn1b (tGFP-tagged) - Mouse sodium channel nonvoltage-gated 1 beta (Scnn1b), (10ug) |
CNY 8,728.00 |
|
MR227329 | Scnn1b (Myc-DDK-tagged) - Mouse sodium channel, nonvoltage-gated 1 beta (Scnn1b) |
CNY 7,128.00 |
|
MR227329L3 | Lenti ORF clone of Scnn1b (Myc-DDK-tagged) - Mouse sodium channel, nonvoltage-gated 1 beta (Scnn1b) |
CNY 8,270.00 |
|
MR227329L4 | Lenti ORF clone of Scnn1b (mGFP-tagged) - Mouse sodium channel, nonvoltage-gated 1 beta (Scnn1b) |
CNY 8,270.00 |