Hspa1l (NM_013558) Mouse Untagged Clone
CAT#: MC219971
Hspa1l (untagged) - Mouse heat shock protein 1-like (Hspa1l), (10ug)
CNY 8,270.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Hsc70t; Msh5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219971 representing NM_013558
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTAATAAAGGAATGGCGATCGGCATCGACTTGGGCACCACCTACTCGTGCGTGGGCGTGTTCC AGCACGGCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACGACCCCTAGCTACGTGGCCTTCAC CGACACCGAGCGCCTCATCGGAGACGCTGCCAAGAACCAGGTGGCCATGAATCCCCAGAACACTGTTTTT GATGCCAAACGTCTAATTGGCAGGAAGTTTAATGATCCTGTTGTGCAGTCAGATATGAAGCTTTGGCCAT TTCAAGTGATCAATGAAGCCGGCAAACCCAAGGTGATGGTGTCCTATAAAGGAGAGAAGAAAGCCTTCTA CCCAGAGGAGATCTCATCCATGGTACTGACGAAGATGAAGGAGACTGCAGAGGCTTTTTTGGGCCACAAT GTCACCAACGCTGTGATCACGGTGCCAGCCTATTTCAATGACTCTCAGCGGCAAGCCACCAAGGATGCAG GTGTCATCGCAGGACTCAATGTGCTGAGAATAATCAATGAGCCCACGGCGGCCGCCATCGCCTACGGCTT GGATAAAGGAAGTCACGGAGAGCGGCACGTGCTCATCTTCGACCTGGGGGGTGGCACGTTCGACGTGTCC ATCCTGACGATCGACGACGGCATCTTCGAGGTGAAGGCCACGGCGGGCGACACGCACCTGGGAGGGGAGG ACTTCGACAACCGGCTGGTGAGCCACTTCGTGGAGGAGTTCAAGAGGAAGCACAAGAAGGACATCAGCCA GAACAAGCGCGCGGTGCGGCGGCTGCGCACTGCGTGTGAGAGGGCCAAGAGGACGCTGTCGTCCAGCACC CAGGCAAACCTGGAGATCGACTCTTTATATGAGGGCATCGACTTCTACACGTCCATCACTAGAGCACGGT TTGAAGAGCTGTGTGCAGACCTATTTAGAGGCACACTTGAGCCCGTGGAAAAGTCTCTTCGGGATGCCAA GATGGATAAGGCTAAAATCCATGACATTGTTCTAGTAGGGGGCTCCACCCGCATCCCAAAGGTGCAAAAA CTGCTTCAGGACTACTTTAATGGACGGGATCTCAACAAGAGTATCAATCCCGATGAGGCAGTCGCCTACG GAGCTGCAGTCCAGGCAGCTATTTTAATGGGCGACAAATCTGAAAAAGTACAGGATTTGCTTTTGTTGGA CGTAGCTCCCCTGTCTCTAGGATTGGAGACAGCTGGGGGTGTGATGACTGTACTGATCAAGCGCAACTCC ACCATCCCCACCAAGCAGACGCAGATCTTCACCACCTACTCGGACAACCAGCCCGGGGTGCTGATCCAGG TGTACGAGGGCGAGAGAGCCATGACGCGCGACAACAACCTGCTGGGGCGCTTTGACTTGACTGGAATACC TCCTGCACCTAGGGGCGTGCCACAGATCGAGGTGACCTTCGACATCGACGCCAACGGTATCCTGAACGTC ACGGCCATGGACAAGAGCACCGGCAAGGCCAACAAGATCACCATCACCAACGACAAGGGTCGCCTGAGCA AGGAGGAGATTGAGCGCATGGTGCAGGAGGCCGAGCGCTACAAAGCGGAGGATGAGGGCCAGAGGGAGAA AATCGCTGCCAAAAATGCCTTAGAATCGTACGCCTTTAATATGAAGAGCGCTGTGGGTGATGAGGGTCTG AAGGACAAGATCAGCGAGTCCGATAAAAAGAAAATACTGGATAAATGCAATGAGGTCCTTTCCTGGCTGG AGGCCAACCAGCTGGCTGAGAAAGATGAGTTTGATCATAAAAGAAAAGAACTGGAAAATATGTGTAATCC GATCATCACAAAACTGTACCAGAGCGGATGCACCGGGCCCACCTGTACGCCAGGGTATACTCCCGGCAGG GCTGCCACAGGCCCTACCATCGAGGAAGTAGATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_013558 |
Insert Size | 1926 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013558.2, NP_038586.2 |
RefSeq Size | 2415 bp |
RefSeq ORF | 1926 bp |
Locus ID | 15482 |
UniProt ID | P16627 |
Gene Summary | Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Positive regulator of PRKN translocation to damaged mitochondria.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG223206 | Hspa1l (tGFP-tagged) - Mouse heat shock protein 1-like (Hspa1l), (10ug) |
CNY 6,180.00 |
|
MR223206 | Hspa1l (Myc-DDK-tagged) - Mouse heat shock protein 1-like (Hspa1l) |
CNY 4,784.00 |
|
MR223206L3 | Lenti ORF clone of Hspa1l (Myc-DDK-tagged) - Mouse heat shock protein 1-like (Hspa1l) |
CNY 7,510.00 |
|
MR223206L4 | Lenti ORF clone of Hspa1l (mGFP-tagged) - Mouse heat shock protein 1-like (Hspa1l) |
CNY 7,510.00 |