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Melk (NM_010790) Mouse Untagged Clone
CAT＃: MC219961
Melk (untagged) - Mouse maternal embryonic leucine zipper kinase (Melk), (10ug)
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI327312; mKIAA0175; MPK38 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219961 representing NM_010790
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGATTATGACGAACTCCTCAAATACTATGAACTATATGAAACGATTGGGACAGGTGGCTTTGCAA AGGTCAAACTGGCCTGCCATGTCCTCACTGGAGAGATGGTAGCTATAAAAATCATGGATAAGAATGCGCT AGGGAGTGATTTGCCCCGAGTCAAAACTGAGATCGATGCGCTGAAGAGTCTGAGACATCAGCACATATGT CAGCTCTACCATGTGCTGGAGACAAAGAACAAAATATTCATGGTTCTGGAGTACTGTCCAGGAGGAGAGC TGTTTGACTACATAATCTCCCAGGATCGCCTGTCGGAAGAGGAGACCCGGGTCGTCTTCCGTCAGATACT GTCTGCAGTTGCGTATGTCCACAGCCAGGGCTATGCCCACAGGGACCTCAAACCAGAAAATTTATTATTT GATGAAAATCATAAGCTAAAGCTGATTGACTTTGGTCTTTGTGCAAAACCCAAGGGCAACAAGGACTACC ATCTGCAGACGTGCTGTGGGAGCCTTGCTTATGCAGCTCCTGAACTAATACAAGGGAAGTCGTACCTTGG ATCAGAGGCAGATGTTTGGAGCATGGGCATCCTCCTGTATGTGCTCATGTGTGGATTTCTACCATTTGAT GATGATAATGTCATGGCTTTGTACAAGAAGATAATGAGAGGGAAATACGAAGTTCCTAAGTGGCTCTCTC CCAGTAGCATTCTGCTTCTCCAGCAGATGTTGCAGGTGGACCCAAAGAAACGGATTTCTATGAGAAATCT CCTGAACCATCCCTGGGTCATGCAAGATTACAGCTGTCCCGTGGAGTGGCAAAGCAAGACTCCTTTGACT CACCTCGATGAGGATTGCGTGACAGAGCTTTCTGTACATCACCGCAGCAGCAGGCAGACAATGGAGGATT TAATTTCGTCGTGGCAGTACGATCACCTCACAGCCACCTACCTTCTGCTTCTAGCCAAGAAGGCCCGGGG GAAGCCGGCTCGTCTACAGCTCCTGTCCTTCTCTTGTGGAACCGCCAGCACCACTCCAAAGTCAAAGAAT CTGAGCCTGGAAGATATGAGCACAAGTGATGATAACTGTGTGGCTGGATTGATAGACTATGAATTGTGTG AAGATAAATTATTAGCTCCCAAGACGCCACAGGTTACCAAACACTTGGCAGAATCAAATCACGCAGCATC TAAATCACCAGCGCCAGGGGTACGCAGAGCAGTGGCAAATAAATTAATGGACAAAGAAAATGTGTGCACT CCCAAGTCTTCTGTGAAGAATGAAGAGCAGTTTGTATTTTCTGAGCCGAAGATTCCAGTTAGTAAGAACC AGTATAAGAGAGAAATACCCGCCTCACCAACCCGTTTCCCAACACCTGCAAAAGCTAGAGCCCAGTGCCT GAGAGAAGCCCCGGTTAGAACACCAGGGAATTCCGCAGGAGCAGACACACTAACGACAGGTGTCATTAGC CCCGAGAGGAGGTGCCGTTCAATGGACGTGGATCTCAACCAGGCACACATGGAGGATACCCCGAAAAAGA AAGGAACCAATGTGTTTGGGAGCCTTGAGAGAGGACTGGATAAGGTTCTCACTGCGCTCACAAGGAACAA GAAGAAGGGCTCTGCCAGAGATGGACCAAGAAAGCGAAAGCTGCACTACAATGTGACTACAACTCGCCTG GTGAACCCCGACCAGCTCCTGAGCGAAATCATGGCTATTCTTCCAAAGAAGAACGTGGACTTCGTACAGA AAGGTTACACTCTAAAGTGTCAAACGCAGTCTGATTTTGGCAAAGTGACAATGCAGTTTGAACTGGAAGT GTGCCAGCTGCAGAGACCTGACGTGGTAGGCATCCGGAGACAGCGGCTGAAGGGTGATGCCTGGGTTTAC AAGAGATTAGTGGAAGATATCTTGTCTGGCTGCAAGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010790 |
| Insert Size | 1932 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010790.2, NP_034920.2 |
| RefSeq Size | 2914 bp |
| RefSeq ORF | 1932 bp |
| Locus ID | 17279 |
| UniProt ID | Q61846 |
| Gene Summary | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209713 | Melk (tGFP-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) |
CNY 6,180.00 |
|
| MR209713 | Melk (Myc-DDK-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) |
CNY 4,792.00 |
|
| MR209713L3 | Lenti ORF clone of Melk (Myc-DDK-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) |
CNY 7,510.00 |
|
| MR209713L4 | Lenti ORF clone of Melk (mGFP-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) |
CNY 7,510.00 |
