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Fbxw7 (NM_080428) Mouse Untagged Clone
CAT＃: MC219910
Fbxw7 (untagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 3, (10ug)
CNY 8,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110001A17Rik; AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxo30; Fbxw6; SEL-10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219910 representing NM_080428
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGTGTGCGTCCCGAGCAGCGTTCTGGTTCTGAGCTGCGTCTGCTGGTGCTGGGGAGTTTTGCTGC CGGTTCCGCTGCCTAATCTTCCTTTTCTGGCGTGCCTGAGCATGTCCACGTTAGAATCTGTGACATACCT ACCTGAAAAGGGGTTATATTGTCAGAGACTGCCAAGCAGCCGGACACACGGGGGCACAGAATCCCTGAAG GGGAAAAATACAGAAAATATGGGTTTCTACGGCACATTAAAAATGATTTTTTACAAAATGAAAAGAAAGT TGGACCATGGTTCTGAAGTTCGTTCCTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGATTATACCAG TACCACTGGCCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTGAGAGCAGCCAATGGGCAA GGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCACCCACAGGCCTTCAAGAGTGGCTGAAAATGTTTC AGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGATGAGCTCATTGACAGCTGTGAACCAACACAAGT GAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAGCGAGACTTCATCTCCTTGCTTCCTAAAGAGTTG GCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTGCAAGCGGCTCAGACTTGTCGATACTGGA GAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAAATGTAAAGAAGAGGGGATTGATGAACCGTTGCA CATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATACACAGCCCATGGAAGAGTGCGTATATCAGACAG CACAGAATTGATACAAACTGGAGACGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGGCATGATGACC ATGTGATCACATGCCTACAGTTTTGTGGCAACCGCATAGTTAGTGGTTCTGATGACAACACTTTAAAAGT TTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTAGTGGGACATACAGGTGGAGTGTGGTCATCACAG ATGAGAGACAATATCATCATCAGTGGATCGACTGACCGGACTCTCAAAGTGTGGAATGCTGAAACTGGAG AGTGTATACATACTTTATATGGGCACACTTCTACTGTACGGTGTATGCATCTCCATGAAAAAAGGGTTGT AAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGATATTGAGACCGGCCAGTGTTTACACGTCCTGATG GGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATGGCAGGAGGGTTGTTAGTGGAGCTTATGATTTTA TGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCTACACACGTTACAGGGACACACTAATAGAGTCTA TTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGAGTCTGGGATGTG GAGACAGGGAATTGTATTCACACGCTAACAGGACACCAGTCATTAACGAGTGGAATGGAACTCAAAGACA ATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACA AACTTTGCAAGGTCCCAGCAAGCATCAGAGCGCTGTGACCTGCTTACAGTTCAACAAGAACTTCGTAATT ACCAGCTCAGACGACGGAACGGTCAAACTCTGGGACTTGAAAACGGGTGAATTTATCCGAAACCTCGTCA CATTGGAGAGTGGGGGGAGCGGGGGAGTTGTGTGGCGGATCAGGGCCTCAAACACAAAGCTGGTGTGTGC AGTCGGGAGTCGGAATGGAACTGAGGAAACCAAGCTCCTGGTGCTGGACTTTGATGTGGACATGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_080428 |
| Insert Size | 1890 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080428.3, NP_536353.2 |
| RefSeq Size | 3728 bp |
| RefSeq ORF | 1890 bp |
| Locus ID | 50754 |
| UniProt ID | Q8VBV4 |
| Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21953459, PubMed:22748924). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:22748924). Mediates ubiquitination and subsequent degradation of CCNE1 and MYC (PubMed:22748924). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1 (By similarity). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity). SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (PubMed:21953459). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed:27238018).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks a few of 5' exons, but has an alternate 5' exon including coding region, as compared to variant 1. The resulting isoform (2) is shorter and has a different N-terminus, as compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225462 | Fbxw7 (tGFP-tagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7) transcript variant 3, (10ug) |
CNY 5,040.00 |
|
| MR225462 | Fbxw7 (Myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 3 |
CNY 4,688.00 |
|
| MR225462L3 | Lenti ORF clone of Fbxw7 (Myc-DDK-tagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 3 |
CNY 6,460.00 |
|
| MR225462L4 | Lenti ORF clone of Fbxw7 (mGFP-tagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 3 |
CNY 6,460.00 |
