Zic5 (NM_022987) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700049L20Rik; Opr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC219831 representing NM_022987 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGCCCCCTTTGAGCAAGAGGAACCCGCCAGCGCTGAGATTAGCGGATTTGGCAACGGCTCAGG CCCAACAGCTTCAGAATATGACAGGCTTCCCGGTGCTGGTCGGCCCGCCCGCCCACTCCCAACGCCGCGC GGTCGCCATGCACCTCCACCCGCGGGACCTAGGCACTGACCCCGGCGTAGCCAGCACTGCGCTCGGACCC GAGCACATGGCACAGGCCAGCGGGCAGGGCCCCTGTCCTCCTTCCCAGGGGCTCCCCGGGCTGTCCCAGG TTCCCGCACCAGCCGCCCGCTCCGTGGCCTCAGGGACTCACCCAGGCGCCCGCACCCACCCCGACGGCGG GGGCAGCAGTGGCGCGCAGGCCTCGGCGCCCCCGCCTCCAGCCCCTCCTCTTCCTCCCTCCCAGTCCTCC TCTCCCCCTCCCCCGCCTCCTCCTCCTCCTGCCCTCTCGGGCTACACTGCCACCAACAGTGGCGGCGGCA GCAGCAGCGGCAAAGGCCACAGCAGGGACTTCGTCCTCCGGAGGGACCTTTCCGCCACGGCCCCCGCGGC GGCCATGCACGGGGCCCCGCTCGGAGGGGAGCAGCGGTCCGGCAGCAGCTCCCCCCAGCACCCGACCCCA CCTCCCCACCCGGCCGGGATGTTCATCTCGGCCAGCGGCACCTACGCGGGCCGGGACGGCGGCGGCTCCG CGCTCTTTCCCGCGCTGCACGACTCTCCCGGGGCTCCCGGTGGCCACCCGCTAAACGGCCAGATGCGCCT TGGGCTGGCGGCCGCCGCGGCGGCTGCGGCCGAGCTTTACGGCCGCGCCGAACCACCCTTCGCTCCGCGC TCAGGGGACGCGCACTATGGGGCGGTGGCGGCCGCCGCGGCCGCCGCCCTGCACGGCTACGGAGCCGTGA ACTTAAACCTGAACCTGGCTGCGGCAGCGGCGGCCGCAGCGGCCGCGGGGCCCGGGCCCCACCTGCAACA CCACGCGCCGCCCCCGGCGCCACCGCCGGCGCCCGCGCCGCACCCGCACCACCCCCACCTCCCGGGGGCG GCCGGGGCCTTCCTGCGCTACATGCGGCAGCCAATCAAGCGGGAGCTCATCTGCAAGTGGCTGGACCCAG AGGAGCTGGCCGGTCCGCCGGCGTCCGCGGACAGCGGCGTCAAGCCCTGCTCCAAAACTTTCGGCACCAT GCACGAGCTGGTGAACCACGTCACAGTGGAGCACGTGGGCGGCCCGGAACAGAGCAGCCACGTCTGCTTT TGGGAGGACTGTCCGCGCGAGGGCAAGCCCTTCAAGGCCAAGTACAAGCTCATCAACCACATCCGCGTGC ACACCGGCGAGAAGCCCTTCCCCTGCCCGTTCCCGGGCTGCGGCAAGGTCTTCGCGCGCTCCGAGAACCT CAAGATCCACAAGCGCACTCATACAGGGGAAAAGCCTTTCAAATGTGAATTTGACGGTTGTGATAGGAAG TTTGCCAATAGCAGCGATCGAAAGAAACACTCCCATGTCCACACCAGCGACAAGCCCTACTACTGTAAGA TTCGAGGCTGTGATAAATCGTACACCCACCCAAGCTCTCTGAGGAAGCACATGAAGATTCACTGCAAGTC CCCCCCACCTTCGCCTGGAGCTCTTGGCTACTCGTCGGTGGGGACTCCCGTGGGTGACCCTTTGTCCCCT GTGCTGGACCCAACCAGGAGTCGATCCAGCACTCTGTCCCCTCAGGTGACCAACCTCAATGAGTGGTATG TTTGCCAGGCCAGTGGGGCCCCCAGCCATCTCCACACACCTTCCAGCAATGGAACCACCTCTGAGTCTGA AGATGAGGAAATGTATGGGAACCCTGAAGTCATGCGGACGATACATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022987
Insert Size	1869 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022987.3, NP_075363.1
RefSeq Size	2383 bp
RefSeq ORF	1869 bp
Locus ID	65100
UniProt ID	Q7TQ40
Gene Summary	Essential for neural crest development, converting cells from an epidermal fate to a neural crest cell fate. Binds to DNA.[UniProtKB/Swiss-Prot Function]

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