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Keap1 (NM_001110305) Mouse Untagged Clone
CAT＃: MC219820
Keap1 (untagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 2, (10ug)
CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | INRF2; mKIAA0132 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219820 representing NM_001110305
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCCGAACCCAAGCTTAGCGGGGCTCCCCGCAGCAGCCAGTTCCTGCCCCTGTGGTCAAAGTGCC CCGAGGGGGCCGGGGACGCAGTGATGTATGCCTCCACGGAGTGCAAGGCAGAGGTGACGCCCTCGCAGGA CGGTAACCGAACCTTCAGCTACACACTAGAGGATCACACCAAGCAGGCTTTTGGCGTCATGAACGAGCTT CGCCTGAGCCAGCAACTCTGTGACGTGACCCTGCAGGTCAAATATGAGGACATCCCAGCTGCCCAATTCA TGGCTCACAAAGTGGTGCTGGCCTCCTCCAGCCCAGTCTTTAAAGCCATGTTCACCAACGGGCTTCGGGA GCAGGGCATGGAGGTGGTGTCCATCGAAGGCATCCACCCTAAGGTCATGGAAAGGCTTATTGAGTTCGCC TACACGGCCTCCATCTCCGTGGGCGAGAAGTGTGTCCTGCACGTGATGAACGGGGCGGTCATGTACCAGA TTGACAGCGTGGTTCGAGCCTGCAGCGACTTCCTCGTGCAGCAGCTGGACCCCAGCAACGCCATTGGCAT CGCCAACTTCGCGGAGCAGATCGGCTGCACTGAACTGCACCAGCGTGCCCGGGAGTATATCTACATGCAC TTCGGGGAGGTGGCCAAGCAGGAGGAGTTCTTCAACCTGTCACACTGCCAGCTGGCCACGCTCATCAGCC GGGATGATCTGAACGTACGCTGCGAGTCCGAGGTGTTCCACGCGTGCATCGACTGGGTCAAATACGACTG CCCGCAGCGGCGCTTCTACGTGCAGGCACTGCTGCGGGCCGTGCGCTGCCATGCGCTCACGCCGCGCTTC CTGCAGACGCAGCTGCAGAAGTGTGAGATCCTGCAGGCCGACGCGCGCTGCAAGGACTACCTGGTGCAGA TATTCCAGGAGCTCACGCTGCACAAGCCCACGCAGGCAGTGCCCTGCCGCGCGCCCAAAGTGGGCCGCCT CATCTACACAGCGGGCGGTTACTTCCGACAGTCGCTCAGCTACCTGGAGGCCTACAACCCGAGCAATGGC TCCTGGCTGCGCCTGGCCGATCTACAGGTGCCGCGCAGTGGGCTGGCAGGCTGCGTGGTGGGTGGGCTGC TATACGCTGTGGGCGGCCGCAACAACTCTCCGGATGGCAACACTGACTCCAGCGCCCTGGACTGCTACAA CCCCATGACCAACCAGTGGTCGCCCTGTGCCTCTATGAGCGTGCCACGCAACCGCATCGGGGTGGGGGTC ATAGATGGCCACATCTACGCAGTCGGGGGTTCCCACGGCTGCATCCACCACAGCAGCGTGGAGAGATATG AGCCAGAGCGGGACGAGTGGCATCTAGTCGCGCCAATGTTGACACGGAGGATTGGCGTGGGCGTGGCAGT GCTCAACCGCTTGCTGTATGCAGTGGGGGGCTTTGACGGGACTAACCGGCTTAACTCCGCAGAATGTTAC TATCCAGAGAGGAATGAGTGGCGGATGATCACACCGATGAATACCATCCGGAGCGGGGCCGGGGTCTGCG TGCTGCACAACTGTATCTATGCAGCAGGGGGCTACGATGGGCAGGACCAGTTGAACAGTGTGGAGCGCTA CGACGTGGAGACAGAGACCTGGACTTTCGTAGCCCCCATGAGGCATCACCGTAGTGCGCTGGGGATTACT GTGCACCAGGGCAAGATCTACGTCCTCGGAGGCTATGATGGCCACACTTTTCTGGACAGTGTGGAATGCT ATGACCCGGACAGTGATACCTGGAGTGAGGTGACCCGCATGACATCTGGCCGCAGCGGGGTGGGTGTGGC CGTCACCATGGAACCCTGTCGGAAGCAAATTGATCAACAAAACTGTACCTGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001110305 |
| Insert Size | 1875 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001110305.1, NP_001103775.1 |
| RefSeq Size | 3434 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 50868 |
| UniProt ID | Q9Z2X8 |
| Gene Summary | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 all encode the same protein. |
Documents
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| FAQs |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225867 | Keap1 (tGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
| MR225867 | Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 2 |
CNY 7,432.00 |
|
| MR225867L3 | Lenti ORF clone of Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 2 |
CNY 6,460.00 |
|
| MR225867L4 | Lenti ORF clone of Keap1 (mGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 2 |
CNY 9,832.00 |
