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Aifm1 (NM_012019) Mouse Untagged Clone
CAT＃: MC219720
Aifm1 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein, (10ug)
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AIF; AIFsh2; Hq; Pdcd8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219720 representing NM_012019
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGGTGTGGAGGCCTGGCGGGTGCTTTCAAGCAGAAACTGGTGCCCTTGGTGCGGACGGTGTACG TCCAGAGGCCGAAACAGAGGAACCGGCTTCCAGGCAACTTGTTCCAGCAATGGCGTGTTCCTCTAGAACT CCAGATGGCAAGACAAATGGCTAGCTCTGGTTCATCAGGGGGCAAAATGGATAATTCTGTGTTAGTCCTT ATTGTGGGCTTATCAACAATAGGAGCTGGTGCATATGCCTACAAAACTATAAAAGAAGACCAAAAAAGAT ACAATGAAAGAGTGATGGGATTAGGACTGTCCCCAGAAGAGAAACAGAGAAGAGCCATTGCCTCCGCTAC AGAGGGAGGCTCAGTTCCTCAGATCAGGGCACCAAGTCACGTCCCTTTCCTGCTGATTGGTGGAGGGACT GCTGCTTTTGCAGCAGCCAGATCCATCCGGGCTCGGGATCCTGGGGCCAGGGTCCTGATTGTATCTGAAG ACCCTGAACTGCCATACATGCGACCTCCTCTTTCAAAAGAATTGTGGTTTTCAGATGATCCAAATGTCAC AAAGACACTGCAATTCAGACAGTGGAATGGAAAAGAGAGAAGCATATATTTCCAGCCACCTTCTTTCTAT GTCTCTGCTCAGGACCTGCCTAATATTGAGAACGGTGGTGTGGCTGTCCTCACTGGGAAAAAGGTAGTAC ATCTGGATGTAAGAGGCAACATGGTGAAACTTAATGATGGCTCTCAGATTACCTTTGAAAAGTGCTTGAT TGCAACGGGAGGCACTCCAAGAAGTCTGTCTGCCATCGATAGGGCTGGAGCAGAGGTGAAGAGTAGAACA ACACTTTTCAGGAAGATTGGAGATTTTAGAGCCTTGGAGAAGATCTCTCGGGAGGTCAAGTCAATTACAG TTATCGGCGGGGGCTTCCTTGGGAGTGAGCTGGCCTGTGCTCTTGGCAGAAAGTCTCAAGCCTCGGGCAT AGAAGTGATCCAGCTGTTCCCTGAGAAAGGAAATATGGGGAAGATCCTTCCTCAATACCTCAGCAACTGG ACCATGGAAAAAGTCAAACGAGAGGGAGTGAAAGTGATGCCCAATGCAATTGTACAATCAGTTGGAGTCA GCGGTGGCAGGTTACTCATTAAGCTGAAAGATGGAAGGAAGGTAGAAACTGACCACATAGTGACAGCTGT GGGCCTAGAGCCCAATGTTGAGTTGGCCAAGACTGGCGGACTGGAAATAGATTCCGATTTTGGTGGCTTC CGGGTAAATGCAGAACTCCAAGCACGTTCTAACATCTGGGTGGCAGGGGATGCTGCATGCTTCTATGATA TAAAGTTGGGTCGAAGGCGAGTAGAGCATCATGATCATGCTGTTGTGAGTGGAAGACTGGCTGGAGAAAA CATGACTGGAGCCGCTAAGCCATACTGGCATCAGTCAATGTTCTGGAGTGATTTGGGTCCTGATGTCGGC TATGAAGCTATTGGTCTGGTGGATAGTAGTTTGCCCACAGTTGGTGTTTTTGCAAAAGCAACTGCACAAG ACAACCCAAAATCTGCCACAGAGCAGTCAGGAACTGGTATCCGTTCGGAGAGTGAGACAGAGTCAGAAGC TTCGGAAATCACAATTCCTCCCAGCGCCCCTGCAGTCCCACAGGTCCCTGTTGAAGGGGAGGACTACGGC AAAGGTGTCATCTTCTACCTCAGGGACAAAGTTGTGGTGGGGATTGTGCTATGGAACGTCTTTAACCGAA TGCCAATTGCAAGGAAGATCATTAAGGACGGTGAGCAACATGAAGATCTCAATGAAGTAGCTAAACTCTT CAACATTCATGAAGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012019 |
| Insert Size | 1839 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012019.3, NP_036149.1 |
| RefSeq Size | 2249 bp |
| RefSeq ORF | 1839 bp |
| Locus ID | 26926 |
| UniProt ID | Q9Z0X1 |
| Gene Summary | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224344 | Aifm1 (tGFP-tagged) - Mouse apoptosis-inducing factor mitochondrion-associated 1 (Aifm1) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 8,432.00 |
|
| MR224344 | Aifm1 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein |
CNY 6,832.00 |
|
| MR224344L3 | Lenti ORF clone of Aifm1 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein |
CNY 7,980.00 |
|
| MR224344L4 | Lenti ORF clone of Aifm1 (mGFP-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein |
CNY 7,980.00 |
