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Itk (NM_010583) Mouse Untagged Clone
CAT＃: MC219702
Itk (untagged) - Mouse IL2-inducible T-cell kinase (Itk), (10ug)
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Emt; Tcsk; Tsk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219702 representing NM_010583
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAACTTCATCCTCCTGGAAGAACAGCTGATCAAGAAGTCCCAACAGAAGAGAAGGACTTCTCCCT CGAATTTTAAAGTTCGTTTCTTTGTCTTAACGAAAGCCAGCCTGGCCTACTTTGAGGACCGCCACGGGAA GAAGCGCACGTTGAAGGGCTCCATTGAACTCTCCAGAATCAAGTGTGTGGAGATTGTCAAGAGTGACATT AGCATCCCGTGCCACTATAAATACCCTTTTCAGGTCGTGCATGACAACTATCTCCTGTATGTGTTTGCTC CAGACTGTGAGAGTCGGCAGCGCTGGGTGCTGACCCTTAAAGAAGAAACGAGGAATAACAACAGCCTGGT ATCCAAGTATCACCCTAATTTCTGGATGGATGGGCGGTGGAGGTGCTGCTCCCAGCTGGAGAAGCCTGCT GTAGGCTGTGCTCCCTACGACCCATCCAAGAATGCTTCAAAGAAGCCTCTTCCTCCTACTCCTGAAGACA ACAGGCGGTCATTTCAGGAACCTGAAGAAACCCTGGTCATTGCCTTGTACGACTACCAAACCAACGACCC TCAGGAGCTCGCACTGCGGTGTGATGAAGAGTACTACCTGCTGGACAGCTCCGAGATCCACTGGTGGAGG GTTCAAGACAAAAATGGGCATGAAGGATATGCACCAAGCAGTTACCTGGTAGAAAAATCTCCAAATAACC TTGAAACCTATGAGTGGTACAATAAAAGCATCAGCCGCGACAAAGCTGAAAAACTTCTTTTGGACACAGG TAAAGAAGGAGCTTTCATGGTCCGAGATTCCAGGACACCCGGGACATACACAGTCTCTGTTTTCACCAAG GCCATCATAAGTGAGAACCCCTGTATAAAACATTATCACATCAAAGAAACAAATGACAGCCCCAAGCGCT ACTACGTGGCTGAGAAGTATGTGTTTGACTCCATCCCTCTCCTCATCCAGTATCACCAGTACAATGGAGG AGGTTTGGTCACTCGACTCCGCTATCCAGTTTGCTCCTGGAGACAAAAAGCCCCTGTCACAGCAGGGCTA AGATATGGGAAGTGGGTGATCCAACCCTCAGAGCTAACGTTCGTGCAGGAGATTGGCAGCGGGCAGTTTG GGCTGGTGCATCTCGGCTACTGGCTCAACAAGGACAAGGTGGCCATCAAGACCATTCAGGAAGGGGCGAT GTCAGAAGAAGACTTTATCGAGGAGGCGGAAGTCATGATGAAACTCTCTCACCCCAAACTGGTGCAGCTC TATGGGGTGTGCCTGGAGCAAGCCCCCATCTGCCTGGTGTTTGAGTTCATGGAGCACGGCTGCCTGTCGG ATTACCTTCGAAGTCAGCGGGGTCTCTTTGCTGCGGAGACCCTGCTGGGCATGTGCCTGGATGTGTGTGA GGGCATGGCCTACCTGGAAAAAGCTTGTGTCATCCACAGAGACCTGGCAGCCAGAAACTGTTTGGTGGGA GAAAACCAGGTCATCAAGGTGTCCGACTTTGGGATGACAAGATTTGTCCTTGATGATCAATATACCAGCT CCACGGGCACCAAATTCCCAGTGAAGTGGGCATCCCCAGAGGTGTTCTCCTTTAGTCGCTATAGCAGCAA GTCAGATGTGTGGTCGTTTGGTGTACTGATGTGGGAAGTCTTCAGTGAAGGCAAAATCCCATACGAAAAC CGAAGCAATTCCGAGGTCGTGGAAGATATCAGCACTGGCTTTCGGTTATACAAGCCCCGCCTGGCCTCCT GTCATGTCTACCAGATCATGAATCATTGCTGGAAAGAGAAACCAGAAGACCGGCCACCCTTCTCCCAGCT GTTAAGCCAGCTGGCTGAAATCGCAGAAGCTGGGCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010583 |
| Insert Size | 1860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010583.3, NP_034713.2 |
| RefSeq Size | 4274 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 16428 |
| UniProt ID | Q03526 |
| Gene Summary | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227110 | Itk (tGFP-tagged) - Mouse IL2-inducible T-cell kinase (Itk), (10ug) |
CNY 5,040.00 |
|
| MR227110 | Itk (Myc-DDK-tagged) - Mouse IL2-inducible T-cell kinase (Itk) |
CNY 4,608.00 |
|
| MR227110L3 | Lenti ORF clone of Itk (Myc-DDK-tagged) - Mouse IL2-inducible T-cell kinase (Itk) |
CNY 6,460.00 |
|
| MR227110L4 | Lenti ORF clone of Itk (mGFP-tagged) - Mouse IL2-inducible T-cell kinase (Itk) |
CNY 6,460.00 |
