Rpn1 (NM_133933) Mouse Untagged Clone
CAT#: MC219644
Rpn1 (untagged) - Mouse ribophorin I (Rpn1), (10ug)
CNY 7,790.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AU018702; D6Wsu137e; Rpn-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219644 representing NM_133933
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGCCGGTCGCCTTGCTGTTGCTGCTGCTGCTATGTCTCGGGGCCTTGGCCCCGACGCCCGGCA GCGCCTCCTCGGAGGCTCCGCCGCTGGTCAACGAGGACGTGAAGCGCACGGTGGACCTGAGCAGCCACCT AGCCAAGGTGACGGCTGAGGTGGTCCTGGTACACCCGGGCGGCGGCTCCACATCACGAGCCAGCTCTTTC GTTCTGGCCCTGGAGCCAGAACTGGAGTCGCGGCTTGCGCACCTAGGCGTGCAGATAAAGGGAGAAGATG AGGAAGACAACAACCTAGAAGTACGAGAAACCAAAATTAAAGGGAAAAGTGGGAGATTTTTCACGGTCAA GCTCCCAGTTGCTCTTGATCCTGGGTCCAAGATCTCGGTCGTTGTGGAAACTGTCTACACCCATGTGCTT CATCCGTATCCGACTCAGATAACTCAGTCAGAGAAACAGTTCGTCGTGTTTGAGGGCAACCATTATTTCT ACTCTCCCTATCCAACAAAGACCCAGACCATGCGAGTGAAACTGGCGTCCCGAAATGTGGAAAGCTATAC CAAGCTGGGGAACCCCTCGAGATCTGAGGACGTCCTTGATTATGGGCCTTTTAAAGACATCCCTGCCTAC AGTCAGGACACTTTCAAAGTACATTACGAGAACAATAGCCCTTTCCTGACCATCACCAGTATGACCCGGG TCATCGAGGTATCTCACTGGGGCAATATTGCTGTGGAAGAGAACGTGGACTTGAAGCACACGGGCGCAGT GCTGAAGGGGCCTTTCTCCCGCTACGATTACCAGAGGCAGCCTGACAGTGGGATCTCCTCCATTCGTTCT TTTAAGACCATCCTTCCTGCTGCCGCCCAGGATGTCTATTACCGGGATGAGATTGGTAATGTTTCCACCA GCCACCTCCTTATCTTGGATGACTCTGTGGAAATGGAAATCCGGCCTCGATTTCCTCTCTTTGGAGGGTG GAAGACACACTACATCGTTGGTTACAACCTCCCAAGCTATGAATACCTCTATAATCTGGGTGACCAGTAT GCACTGAAGATGCGGTTTGTAGACCACGTGTTTGATGAGCAAGTGATAGATTCTCTGACAGTGAAGATCA TCTTGCCCGAGGGAGCCAAGAACATCCAGGTGGACAGTCCCTACGATATCAGCCGGGCCCCAGACGAGCT GCATTATACCTACCTAGACACGTTCGGCCGCCCGGTGATTGTGGCTTACAAGAAGAATCTCGTCGAGCAA CACATTCAGGACATTGTGGTGCACTACACGTTCAACAAGGTTCTCATGCTGCAGGAGCCGCTGCTGGTCG TGGCCGCCTTCTACATCCTGTTCTTCACCGTCATCATCTACGTCCGTCTGGACTTTTCCATCACCAAGGA TCCAGCTGCAGAAGCCAGGATGAAAGTGGCCTGTATCACAGAGCAGGTCTTAACCCTGGTCAACAAGAGG CTGGGCCTCTACCGTCACTTTGATGAGACTGTCAATAGATACAAGCAGTCCCGGGATATCTCTACCCTCA ACAGTGGCAAGAAGAGCCTAGAGACAGAGCACAAGGCTGTGACCAGCGAGATTGCTGTACTGCAGTCTCG GCTGAAGACGGAGGGCTCAGACCTGTGTGACAGGGTGAGTGAGATGCAGAAGCTGGACGCCCAGGTCAAG GAGCTGGTCCTGAAGTCGGCGGTAGAAGCAGAGAGGCTGGTGGCTGGCAAGCTCAAGAAGGATACGTACC TGGAAAACGAGAAGCTCAGCTCAGGAAAACGCCAGGAGCTGGTCACCAAGATCGACCACATCCTGGATGC TCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_133933 |
Insert Size | 1827 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_133933.4, NP_598694.3 |
RefSeq Size | 3642 bp |
RefSeq ORF | 1827 bp |
Locus ID | 103963 |
UniProt ID | Q91YQ5 |
Gene Summary | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG209378 | Rpn1 (tGFP-tagged) - Mouse ribophorin I (Rpn1) |
CNY 5,890.00 |
|
MR209378 | Rpn1 (Myc-DDK-tagged) - Mouse ribophorin I (Rpn1) |
CNY 4,528.00 |
|
MR209378L3 | Lenti ORF clone of Rpn1 (Myc-DDK-tagged) - Mouse ribophorin I (Rpn1) |
CNY 7,220.00 |
|
MR209378L4 | Lenti ORF clone of Rpn1 (mGFP-tagged) - Mouse ribophorin I (Rpn1) |
CNY 7,220.00 |