Rpn1 (NM_133933) Mouse Untagged Clone

CAT#: MC219644

Rpn1 (untagged) - Mouse ribophorin I (Rpn1), (10ug)



  "NM_133933" in other vectors (4)

CNY 7,790.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AU018702; D6Wsu137e; Rpn-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219644 representing NM_133933
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCGCCGGTCGCCTTGCTGTTGCTGCTGCTGCTATGTCTCGGGGCCTTGGCCCCGACGCCCGGCA
GCGCCTCCTCGGAGGCTCCGCCGCTGGTCAACGAGGACGTGAAGCGCACGGTGGACCTGAGCAGCCACCT
AGCCAAGGTGACGGCTGAGGTGGTCCTGGTACACCCGGGCGGCGGCTCCACATCACGAGCCAGCTCTTTC
GTTCTGGCCCTGGAGCCAGAACTGGAGTCGCGGCTTGCGCACCTAGGCGTGCAGATAAAGGGAGAAGATG
AGGAAGACAACAACCTAGAAGTACGAGAAACCAAAATTAAAGGGAAAAGTGGGAGATTTTTCACGGTCAA
GCTCCCAGTTGCTCTTGATCCTGGGTCCAAGATCTCGGTCGTTGTGGAAACTGTCTACACCCATGTGCTT
CATCCGTATCCGACTCAGATAACTCAGTCAGAGAAACAGTTCGTCGTGTTTGAGGGCAACCATTATTTCT
ACTCTCCCTATCCAACAAAGACCCAGACCATGCGAGTGAAACTGGCGTCCCGAAATGTGGAAAGCTATAC
CAAGCTGGGGAACCCCTCGAGATCTGAGGACGTCCTTGATTATGGGCCTTTTAAAGACATCCCTGCCTAC
AGTCAGGACACTTTCAAAGTACATTACGAGAACAATAGCCCTTTCCTGACCATCACCAGTATGACCCGGG
TCATCGAGGTATCTCACTGGGGCAATATTGCTGTGGAAGAGAACGTGGACTTGAAGCACACGGGCGCAGT
GCTGAAGGGGCCTTTCTCCCGCTACGATTACCAGAGGCAGCCTGACAGTGGGATCTCCTCCATTCGTTCT
TTTAAGACCATCCTTCCTGCTGCCGCCCAGGATGTCTATTACCGGGATGAGATTGGTAATGTTTCCACCA
GCCACCTCCTTATCTTGGATGACTCTGTGGAAATGGAAATCCGGCCTCGATTTCCTCTCTTTGGAGGGTG
GAAGACACACTACATCGTTGGTTACAACCTCCCAAGCTATGAATACCTCTATAATCTGGGTGACCAGTAT
GCACTGAAGATGCGGTTTGTAGACCACGTGTTTGATGAGCAAGTGATAGATTCTCTGACAGTGAAGATCA
TCTTGCCCGAGGGAGCCAAGAACATCCAGGTGGACAGTCCCTACGATATCAGCCGGGCCCCAGACGAGCT
GCATTATACCTACCTAGACACGTTCGGCCGCCCGGTGATTGTGGCTTACAAGAAGAATCTCGTCGAGCAA
CACATTCAGGACATTGTGGTGCACTACACGTTCAACAAGGTTCTCATGCTGCAGGAGCCGCTGCTGGTCG
TGGCCGCCTTCTACATCCTGTTCTTCACCGTCATCATCTACGTCCGTCTGGACTTTTCCATCACCAAGGA
TCCAGCTGCAGAAGCCAGGATGAAAGTGGCCTGTATCACAGAGCAGGTCTTAACCCTGGTCAACAAGAGG
CTGGGCCTCTACCGTCACTTTGATGAGACTGTCAATAGATACAAGCAGTCCCGGGATATCTCTACCCTCA
ACAGTGGCAAGAAGAGCCTAGAGACAGAGCACAAGGCTGTGACCAGCGAGATTGCTGTACTGCAGTCTCG
GCTGAAGACGGAGGGCTCAGACCTGTGTGACAGGGTGAGTGAGATGCAGAAGCTGGACGCCCAGGTCAAG
GAGCTGGTCCTGAAGTCGGCGGTAGAAGCAGAGAGGCTGGTGGCTGGCAAGCTCAAGAAGGATACGTACC
TGGAAAACGAGAAGCTCAGCTCAGGAAAACGCCAGGAGCTGGTCACCAAGATCGACCACATCCTGGATGC
TCTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133933
Insert Size 1827 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133933.4, NP_598694.3
RefSeq Size 3642 bp
RefSeq ORF 1827 bp
Locus ID 103963
UniProt ID Q91YQ5
Gene Summary Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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