Xrcc6 (NM_010247) Mouse Untagged Clone

CAT#: MC219608

Xrcc6 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 6 (Xrcc6), (10ug)



  "NM_010247" in other vectors (4)

CNY 4,536.00

CNY 7,790.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 70kDa; G22p1; Ku70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219608 representing NM_010247
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGAGTGGGAGTCCTACTACAAAACTGAGGGCGAGGAAGAGGAAGAGGAGGAGGAGAGCCCCGACA
CAGGTGGAGAATATAAATATTCAGGAAGAGATAGCTTGATTTTTCTGGTTGATGCCTCCAGGGCTATGTT
CGAATCTCAGGGTGAAGATGAACTCACACCTTTTGATATGAGCATCCAGTGTATCCAGAGTGTGTACACC
AGTAAGATCATAAGCAGCGATCGGGATCTCCTGGCAGTGGTGTTCTATGGAACCGAGAAAGATAAAAATT
CAGTGAATTTCAAAAATATTTATGTCTTACAAGATTTGGACAACCCAGGCGCTAAGCGAGTGCTAGAGCT
CGACCAGTTTAAGGGACAACAGGGGAAGAAGCACTTCCGAGACACGGTTGGCCATGGGTCTGACTACTCT
TTGAGTGAAGTGCTCTGGGTCTGTGCCAACCTCTTCAGCGACGTCCAGCTCAAGATGAGTCACAAGAGGA
TCATGCTGTTCACCAATGAAGACGACCCCCATGGCCGTGACAGTGCTAAAGCCAGCCGGGCCAGGACCAA
AGCCAGCGACCTCCGGGACACTGGGATCTTCCTTGACTTGATGCATCTGAAGAAGCCAGGAGGCTTTGAT
GTATCCGTGTTCTACAGGGACATCATCACCACCGCTGAGGACGAGGACCTTGGGGTTCACTTCGAGGAGT
CAAGCAAGCTGGAAGACCTGCTAAGGAAGGTTCGAGCCAAGGAGACCAAAAAGCGAGTTCTGTCCAGGTT
AAAGTTTAAGCTCGGTGAAGACGTAGTACTCATGGTGGGCATTTATAACTTGGTCCAGAAAGCTAACAAG
CCTTTTCCAGTGAGACTCTATCGGGAAACAAATGAACCAGTGAAAACCAAGACAAGGACTTTTAATGTAA
ACACCGGCAGTCTACTCCTGCCTAGTGACACCAAGCGGTCTCTGACTTATGGGACACGTCAGATTGTGCT
GGAGAAAGAGGAGACAGAGGAGCTGAAGCGGTTTGATGAGCCAGGTTTGATCCTCATGGGCTTTAAGCCC
ACGGTGATGCTGAAGAAGCAGCACTACCTGAGGCCCTCTCTGTTCGTGTACCCGGAGGAGTCCCTGGTCA
GTGGGAGCTCAACCTTGTTCAGCGCTCTGCTCACCAAGTGTGTGGAGAAGGAGGTCATAGCAGTGTGTAG
ATACACACCCCGGAAGAACGTCTCCCCGTATTTTGTGGCTTTGGTGCCCCAGGAAGAGGAGCTGGATGAT
CAGAACATTCAGGTGACTCCAGGAGGCTTCCAGCTTGTCTTCCTCCCTTATGCCGATGACAAGCGGAAGG
TGCCCTTTACTGAGAAGGTGACGGCCAACCAGGAGCAGATAGACAAGATGAAGGCCATTGTTCAAAAGCT
CCGCTTCACATACAGGAGCGACAGTTTTGAGAATCCAGTCCTGCAGCAGCACTTCCGCAACCTGGAGGCC
CTAGCTTTGGACATGATGGAGTCGGAGCAAGTGGTAGATCTGACACTACCCAAGGTTGAAGCCATAAAGA
AAAGACTGGGTTCCCTGGCAGATGAGTTTAAAGAACTTGTCTATCCTCCAGGTTATAATCCCGAGGGAAA
AGTTGCCAAGAGAAAACAAGATGATGAAGGTTCTACGAGTAAAAAGCCCAAGGTAGAGTTATCAGAAGAA
GAGCTGAAGGCCCATTTTCGTAAGGGCACACTGGGTAAGCTCACTGTACCTACACTGAAGGACATATGCA
AGGCTCATGGGCTTAAGAGTGGGCCGAAGAAGCAGGAACTGCTAGATGCTCTTATCAGACACTTGGAGAA
GAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010247
Insert Size 1827 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010247.2, NP_034377.2
RefSeq Size 2113 bp
RefSeq ORF 1827 bp
Locus ID 14375
UniProt ID P23475
Gene Summary Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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