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Ift74 (NM_026319) Mouse Untagged Clone
CAT＃: MC219528
Ift74 (untagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74), (10ug)
CNY 6,712.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700029H06Rik; b2b796Clo; Ccdc2; Cmg1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_026319 |
| Insert Size | 1803 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_026319.2, NP_080595.2 |
| RefSeq Size | 2128 bp |
| RefSeq ORF | 1803 bp |
| Locus ID | 67694 |
| UniProt ID | Q8BKE9 |
| Gene Summary | Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220468 | Ift74 (tGFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74), (10ug) |
CNY 6,560.00 |
|
| MR220468 | Ift74 (Myc-DDK-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74) |
CNY 6,696.00 |
|
| MR220468L3 | Lenti ORF clone of Ift74 (Myc-DDK-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74) |
CNY 7,890.00 |
|
| MR220468L4 | Lenti ORF clone of Ift74 (mGFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74) |
CNY 7,890.00 |
