Epb41 (NM_001128607) Mouse Untagged Clone

CAT#: MC219468

Epb4.1 (untagged) - Mouse erythrocyte protein band 4.1 (Epb4.1), transcript variant 3, (10ug)



  "NM_001128607" in other vectors (3)

CNY 7,510.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4.1R; AI415518; D4Ertd442e; Elp-1; Elp1; Epb4.1; mKIAA4056
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219468 representing NM_001128607
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTGTAAGGTCTCCTTGTTGGATGACACGGTCTACGAATGTGTTGTAGAGAAACATGCTAAGGGAC
AAGACTTGCTGAAGCGAGTGTGCGAGCACCTCAACCTTTTGGAAGAAGACTACTTTGGTTTAGCCCTGTG
GGACAGCGCAACCTCTAAGACATGGCTGGATTCTGCCAAAGAAATAAAAAAGCAGGTTCGAGGTGTTCCT
TGGAATTTCACATTTAATGTGAAGTTTTATCCACCCGACCCAGCACAATTAACAGAAGACATAACAAGAT
ACTATTTATGTCTTCAGCTTCGGCAGGACATTGTTGCTGGACGTCTGCCCTGTTCCTTTGCAACTTTAGC
CCTACTCGGGTCTTACACGATCCAGTCTGAGCTGGGAGACTATGACCCAGAACTGCACGGCATGGATTAT
GTTAGTGATTTTAAACTGGCTCCAAATCAGACCAAGGAACTTGAAGAAAAGGTCATGGAATTGCATAAAT
CATACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAATTTCTTGAGAATGCCAAAAAGTTGTCCATGTA
TGGAGTTGATCTTCACAAAGCAAAGGACTTGGAGGGAGTGGACATTATTCTCGGCGTCTGCTCCAGCGGC
CTTCTGGTTTACAAAGACAAGTTGAGAATTAACCGCTTTCCTTGGCCCAAAGTGCTAAAGATTTCTTACA
AACGCAGCAGCTTCTTCATCAAGATCCGGCCTGGAGAGCAAGAACATTATGAAAGTACCATCGGCTTCAA
GCTCCCCAGTTATCGAGCGGCCAAGAAACTATGGAAGGTCTGTGTGGAGCATCACACGTTCTTCAGACTC
ACCTCTACAGACACCATCCCCAAAAGCAAGTTTCTTGCCCTGGGATCCAAATTCCGATACAGTGGCCGGA
CTCAAGCTCAGACCAGGCAAGCCAGTGCCCTGATTGACAGGCCTGCTCCACACTTTGAGCGGACAGCAAG
CAAGCGGGCGTCCAGGAGCCTCGATGGAGCAGCAGCTGCTGAGTCCACAGACCGAAGTCCTCGGCCCACC
TCTGCCCCAGCCATTGCTCAGAGTCAGGTCACAGAAGGGCCAGGGGCACCTATCAAAAAAACACCAAAGG
AAGCTGTGAAGGTTGAAGAGAAGCGGGGAGAAGAGCCGGCTGAGCCCGCTGAGCCGGAGCCCACAGAAGC
ATGGAAGGATTTAGACAAGAGTCAAGAAGAGATCAAAAAGCACCATGCCAGCATCAGTGAACTGAAAAAG
AACTTTATGGAATCGGTACCCGAACCACGGCCCAGCGAGTGGGACAAGCGCTTATCTACACACTCACCCT
TCCGGACTCTTAACATCAACGGGCAAGTCCCTACTGGAGATGGACCTCCTCTGGTAAAGACTCAAACTGT
CACCATCTCAGATACTGCCAATGCTGTGAAAAGTGAAATCCCAACCAAAGATGTCCCTATTGTCCACACT
GAGACCAAGACCATCACCTATGAAGCTGCCCAGACTGAGGACAGCAATGGGGACTTAGACCCTGGAGTCT
TGCTGACAGCCCAGACCATCACATCCGAGACCACAAGTAGTACAACCACGACACAGATTACCAAGACTGT
AAAAGGTGGGATTTCTGAGACCCGGATCGAGAAGAGAATTGTGATCACAGGAGATGCCGATATCGACCAT
GATCAGGTCCTTGTACAAGCCATCAAGGAAGCCAAGGAGCAGCATCCAGACATGTCAGTGACCAAGGTGG
TCGTCCACCAGGAGACAGAGATCTCTGAGGAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001128607
Insert Size 1785 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001128607.1, NP_001122079.1
RefSeq Size 5067 bp
RefSeq ORF 1785 bp
Locus ID 269587
UniProt ID P48193
Gene Summary Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) lacks multiple in-frame exons, uses an alternate 5' splice site, and uses a different 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream ATG and an isoform (3) that is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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