Rec8 (NM_020002) Mouse Untagged Clone
CAT#: MC219409
Rec8 (untagged) - Mouse REC8 homolog (yeast) (Rec8), (10ug)
CNY 7,510.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | mrec; Rec8L1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219409 representing NM_020002
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTACTATCCTAACGTGCTTCAGCGCCACACAGGCTGCTTCGCCACCATCTGGCTGGCAGCAACCC GAGGCAGCCGTTTGGTGAAGCGTGAATACCTAAATGTGAATGTGGTAAAGACCTGCGAGGAAATCCTCAA TTATGTGCTGGTAAGAGTGCAACCTCCGGTGGCCGGGCTGCCCCGCCCCCGCTTCTCCCTCTATCTCTCC GCCCAGCTTCAGATTGGTGTGATAAGGGTCTACTTTCAACAGTGCCAGTACCTTGTGGAAGACATCCAGC ATATCCTGGAGCACCTACATCGTGCCCAGCTGCGGATTCGCATTGATATGGAGGAGGCTGACCTACCTAG CTTGCTTCTTCCCAACTGCCTGGCCATGATGGAGACGCTGGAAGATGCCCCAGAACCCTTTTTTGGGAAG ATGTCTGTGGATCCTAGACTTCCCAGTCCTTTTGATATTCCTCAGATTCGACACCTTTTAGAGGCTGCAA CCCCGGAAAAAACTCGTAAGGAGACCCTTCCTGAAGCCACTCCAGACCCCAGGAAGCCTGACAGGACCTT GGCCACCGTGCAGTCCCCTGAGGTCATCACCTTACAGGAGGCAGAGCCCATCCGCATGCTGCAGATTGAA GGAGAGCAGGATCTCCCAGAGATCAGTCGAGGAGACTTAGAACTGCTGATCGCAGAGAAAGATGACGCCA TCTTGCTAGAGGAACGTCAGAGAGGCAGGCTTCTCCGACAGCGGAGAGCTTCACTACCACTGGATGAATC CAGGGAAGAACCCCGGGCCCTAGAAGGTGCTGGTTTGGTCTCGGCACTCTCACCTCCAGCTCCTGCGCAG GTGGAAGGGATACAAGAAGCACTACCAGGCCAGGTCTTCCCTCCTGAGGTACAGAAGATGACAGGCTGGG AGCCTGGGGCCCTACTCACTGAGGTGACCCCACCTCAGGAGCTGCGGCTGCCTGCCCCACCCAGTACAGA GAAGAGGCTCCCGTCTCTTCAGAGGCCACTTCCCCGCCGACACCGGCGGCGCCAGTTATTATTCTGGGAC AAGGAGACCCAGATCTCTCGGGAGAAGTTTGAGGAACAACTGCAGACTGGGGCTCACTGCTGGGAGTATC CAGTGGCCCAGCCTCCCAAGAGGATGCTCACAAGCCCAGCGGAGCTCTTCAGAACCCCAACTCTCTCTGG CTGGCTACCCCCAGAACTCCTAGGTTTATGGACACACTGTGCCCAGGTCCCCCAAAGAATGCTCAGACAA AGGCCACAGCTGGAGACTGAGGAGACAGTGGAGGAAGAGAGAGCAGCAGATGAGGAAGAGAGGAGAAAGA CTGAAGCTCTGAGCGAGATCGAGGTTCTGAGGGAGGCCCAGGAGCCCAGTGGTCCCCTCATGCTGTCTTC AGAGCTCTCCCTAGAAGCAGCAGAAGATGAAAAGTCTCGTACTAGCCTCATCCCCCCAGAATGGTGGGCC TGGTCTGAGGAGGGGCAGCCCGAGCCCCCTGCACTGCCCATGCTGCCTGAACTTCCTGAAGTGCCCATGG AGATGCCCCCAAGGCCTGAACTCTCTTCAGAGGCTGTGCTCAGGGCAGTAGCACTGAAACTGCAGGCCAA CAAGGAGCTGGACTTCAGCAGCCTGGTGCCCCCTCTCAGCCCCCGAAAGTTGGCTTCTCGGGTCTTCTAT CTGCTCTTGGTGCTGTCCACACAGAAGATCCTTCTTGTGGAACAACAGAAGCCATATGGGCCCCTCCTGA TTCGTCCTGGACCCAAATTCCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_020002 |
Insert Size | 1776 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_020002.3, NP_064386.2 |
RefSeq Size | 2055 bp |
RefSeq ORF | 1776 bp |
Locus ID | 56739 |
UniProt ID | Q8C5S7 |
Gene Summary | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG209218 | Rec8 (tGFP-tagged) - Mouse REC8-like 1 (yeast) (Rec8L1) |
CNY 8,208.00 |
|
MR209218 | Rec8 (Myc-DDK-tagged) - Mouse REC8 homolog (yeast) (Rec8) |
CNY 6,608.00 |
|
MR209218L3 | Lenti ORF clone of Rec8 (Myc-DDK-tagged) - Mouse REC8 homolog (yeast) (Rec8) |
CNY 7,790.00 |
|
MR209218L4 | Lenti ORF clone of Rec8 (mGFP-tagged) - Mouse REC8 homolog (yeast) (Rec8) |
CNY 7,790.00 |