Gpr161 (NM_001081126) Mouse Untagged Clone
CAT#: MC219021
Gpr161 (untagged) - Mouse G protein-coupled receptor 161 (Gpr161), (10ug)
CNY 4,160.00
CNY 7,130.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Gm208; Gm208Gpr; vl |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219021 representing NM_001081126
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGCACCACACCACACACACTCTCTTGGCTGTATTTCCTGTCGTCCAGCATGCTCTGCTCACCG CATCCCGAGGGGCACTGACCATGAGCCTCAACTCCTCCCTCAGCTACAGGAAGGAACTGAGCAACCTCAC TGCAACAGAGGGTGGTGAAGGGGGTGCCGTCTCTGAGTTCATCGCCATCATCATCATCACTGTCCTCGTC TGCCTGGGCAACCTGGTCATTGTAGTCACCTTGTACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGT TCGTCTTCAGCCTGACCTTGTCCAACTTCCTGTTGTCGGTGCTGGTGCTGCCGTTCGTGGTGACTAGCTC CATCCGGAGGGAATGGATTTTCGGCGTGGTTTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGC TCAGCCAGCATGCTCACGCTTGGGGTCATTGCCATCGATCGCTACTACGCCGTCCTGTATCCAATGGTGT ACCCCATGAAGATCACAGGGAACCGAGCTGTGATGGCTCTCGTCTACATCTGGCTCCACTCTCTCATTGG CTGTCTGCCGCCCCTATTTGGTTGGTCATCGGTGGAGTTTGATGAGTTCAAGTGGATGTGTGTGGCCGCG TGGCACCAGGAACCCGGCTACACCATTTTCTGGCAGATCTGGTGTGCCCTGTTCCCCTTTCTCATCATGC TAGTGTGCTACGGTTTCATCTTCCGGGTGGCCAGGGTCAAGGCCCGAAAGGTGCACTGTGGCACGGTGGT CACTGTGGAGGAGGACTCTCAGAGGAGCGGGAGGAAGAATTCTAGTACCTCCACTTCCTCCTCCGGCAGT AGGAGGAATGCCCTTCAAGGAGTGGTCTATTCAGCTAACCAGTGCAAAGCCCTCATCACCATCCTGGTGG TCATTGGCGCCTTCATGGTCACCTGGGGCCCCTACATGGTTGTCATTACCTCAGAGGCACTCTGGGGGAA GAACTGTGTCTCCCCAACCCTGGAGACTTGGGCCACATGGCTGTCCTTTACCAGTGCCATCTGCCACCCT CTGATCTACGGACTCTGGAACAAGACTGTGCGCAAGGAGCTCCTGGGCATGTGCTTTGGGGACCGTTATT ACCGGGAATCCTTTGTGCAGCGACAGAGGACCTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACTT GGGTCTGTCCCCACATCTCACGGCGCTCATGGCAGGCGGACAGTCCCTGGGACACAGCAGCAGCACCGGT GACACAGGCTTCAGCTACTCTCAGGATTCAGGGACGGATGTGATGCTGTTGGAAGATGGCACTTCTGAGG ACAACCCTCCCCAGCACTGCACTTGCCCACCCAAGAGGAGGAGCTCCGTGACATTTGAGGACGAAGTGGA ACAGATCAAAGAGGCCGCCAAGAACTCTCTTCTTCATGTGAAAGCTGAAGTACACAAATCCTTGGACAGT TACGCAGCCAGCTTGGCTAAAGCCATTGAGGCTGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTCTGC CAGGGGTCTTGTTCACAGCACGGACCGTCCCAGGGGCTGGCTTTGGGGGCCGTCGAGGCAGCAGAACTCT TGTGAATCAGAGGCTGCAGCTACAGAGCATCAAAGAAGGCAATGTCTTAGCTGCAGAACAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_001081126 |
Insert Size | 1677 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001081126.1, NP_001074595.1 |
RefSeq Size | 1943 bp |
RefSeq ORF | 1677 bp |
Locus ID | 240888 |
UniProt ID | B2RPY5 |
Gene Summary | Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224248 | Gpr161 (tGFP-tagged) - Mouse G protein-coupled receptor 161 (Gpr161), (10ug) |
CNY 5,040.00 |
|
MR224248 | Gpr161 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 161 (Gpr161) |
CNY 4,160.00 |
|
MR224248L3 | Lenti ORF clone of Gpr161 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 161 (Gpr161) |
CNY 6,460.00 |
|
MR224248L4 | Lenti ORF clone of Gpr161 (mGFP-tagged) - Mouse G protein-coupled receptor 161 (Gpr161) |
CNY 6,460.00 |