Gpr161 (NM_001081126) Mouse Untagged Clone

CAT#: MC219021

Gpr161 (untagged) - Mouse G protein-coupled receptor 161 (Gpr161), (10ug)



  "NM_001081126" in other vectors (4)

CNY 4,160.00

CNY 7,130.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gm208; Gm208Gpr; vl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219021 representing NM_001081126
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCGCACCACACCACACACACTCTCTTGGCTGTATTTCCTGTCGTCCAGCATGCTCTGCTCACCG
CATCCCGAGGGGCACTGACCATGAGCCTCAACTCCTCCCTCAGCTACAGGAAGGAACTGAGCAACCTCAC
TGCAACAGAGGGTGGTGAAGGGGGTGCCGTCTCTGAGTTCATCGCCATCATCATCATCACTGTCCTCGTC
TGCCTGGGCAACCTGGTCATTGTAGTCACCTTGTACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGT
TCGTCTTCAGCCTGACCTTGTCCAACTTCCTGTTGTCGGTGCTGGTGCTGCCGTTCGTGGTGACTAGCTC
CATCCGGAGGGAATGGATTTTCGGCGTGGTTTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGC
TCAGCCAGCATGCTCACGCTTGGGGTCATTGCCATCGATCGCTACTACGCCGTCCTGTATCCAATGGTGT
ACCCCATGAAGATCACAGGGAACCGAGCTGTGATGGCTCTCGTCTACATCTGGCTCCACTCTCTCATTGG
CTGTCTGCCGCCCCTATTTGGTTGGTCATCGGTGGAGTTTGATGAGTTCAAGTGGATGTGTGTGGCCGCG
TGGCACCAGGAACCCGGCTACACCATTTTCTGGCAGATCTGGTGTGCCCTGTTCCCCTTTCTCATCATGC
TAGTGTGCTACGGTTTCATCTTCCGGGTGGCCAGGGTCAAGGCCCGAAAGGTGCACTGTGGCACGGTGGT
CACTGTGGAGGAGGACTCTCAGAGGAGCGGGAGGAAGAATTCTAGTACCTCCACTTCCTCCTCCGGCAGT
AGGAGGAATGCCCTTCAAGGAGTGGTCTATTCAGCTAACCAGTGCAAAGCCCTCATCACCATCCTGGTGG
TCATTGGCGCCTTCATGGTCACCTGGGGCCCCTACATGGTTGTCATTACCTCAGAGGCACTCTGGGGGAA
GAACTGTGTCTCCCCAACCCTGGAGACTTGGGCCACATGGCTGTCCTTTACCAGTGCCATCTGCCACCCT
CTGATCTACGGACTCTGGAACAAGACTGTGCGCAAGGAGCTCCTGGGCATGTGCTTTGGGGACCGTTATT
ACCGGGAATCCTTTGTGCAGCGACAGAGGACCTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACTT
GGGTCTGTCCCCACATCTCACGGCGCTCATGGCAGGCGGACAGTCCCTGGGACACAGCAGCAGCACCGGT
GACACAGGCTTCAGCTACTCTCAGGATTCAGGGACGGATGTGATGCTGTTGGAAGATGGCACTTCTGAGG
ACAACCCTCCCCAGCACTGCACTTGCCCACCCAAGAGGAGGAGCTCCGTGACATTTGAGGACGAAGTGGA
ACAGATCAAAGAGGCCGCCAAGAACTCTCTTCTTCATGTGAAAGCTGAAGTACACAAATCCTTGGACAGT
TACGCAGCCAGCTTGGCTAAAGCCATTGAGGCTGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTCTGC
CAGGGGTCTTGTTCACAGCACGGACCGTCCCAGGGGCTGGCTTTGGGGGCCGTCGAGGCAGCAGAACTCT
TGTGAATCAGAGGCTGCAGCTACAGAGCATCAAAGAAGGCAATGTCTTAGCTGCAGAACAGAGATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001081126
Insert Size 1677 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081126.1, NP_001074595.1
RefSeq Size 1943 bp
RefSeq ORF 1677 bp
Locus ID 240888
UniProt ID B2RPY5
Gene Summary Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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