Rad18 (NM_001167730) Mouse Untagged Clone

CAT#: MC218972

Rad18 (untagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 1, (10ug)



  "NM_001167730" in other vectors (4)

CNY 7,130.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2810024C04Rik; Rad18sc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218972 representing NM_001167730
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTGATGAAGACAATAGATGACTTGCTGC
GCTGTGGGATTTGCTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC
ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA
GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC
AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA
TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGCGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA
ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA
GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGGCTCTCGGATGCTAATGG
TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC
ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTTTATCACGTGAAGAGAAGAAGGAGAGCCTGC
GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA
GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAG
TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATCGTCCAAGAAA
TTGAAAGCATGGAGAAGACCAGGATGCGCCTTGAAGCAAGTAAACTCAATGAAAATGTCATGGTTTTTAC
AAAGAACCAAACAGAGAAGGAAATTGAGGAAGTTCACAGTGAATATCATGCTGGCAAACAGGTAGCACAG
GGCTGTGTGAGCGAAAACAAGATGAGAAGCACCATAGTCTGCTGGCAGTTATCAGGTAAAAAGCACCAGA
ATGCATTCCAGCTTCTGGTGGATCAGGCCAAAAAAGGGTATAAGAAAACTGGCAGAGTTTCACAAGCTGC
AGCGATGAGAACAGATGAACCTGCAGAGACACTGCCGTCGATGAGAACAGATGAACCTGCAGAGACACTG
CCGTCGATGAGAACAGATGAACCTGCAGAGACACTGCCGTTGATGAGAGCAGATGAACCTGCAGAAACAC
TGCCGTCTGAGTGTATCGCACAAGAAGATAATGTGAGCTTCTCAGATACTGTCTCAGTAACAAACCACTT
TCCTCAGCCCCAGCTGGACTCGCCAGGCCCGTCGGAGCCTGAGAGGCCGGATGATTCTTCTAGTTGTACT
GATATTCTTTTCTCCTCGGACTCAGACTCATGCAATAGTTCCAGTTCAGACATCATAAGAGATCTTCTGG
AAGAAGAGGAAGCCTGGGAAGCGGCTCACAAAAATGATCAAAACAGAGAAGTCAGCCCACAACAGACTCG
CCGCACTAGAGCCAGTGAATGTGTTGAGATTGAACCAAGAAACAAGCGGAATAAGAATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001167730
Insert Size 1671 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001167730.1, NP_001161202.1
RefSeq Size 2701 bp
RefSeq ORF 1671 bp
Locus ID 58186
Gene Summary E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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