Zfp353 (NM_153096) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC218887 representing NM_153096 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAAACACAGGTGCTTCCAAGCCAAGACCCTGTAATACCCAGCCTTCCCCTACGGAGTCCAGCC AGCTTGTCTCAGGTCAGAGCTCAGAGATTCTTACCTCGAAACAAACAGTGATGGCATCTGCATCCTGTAG CACACAGGAGTCAACCTATACTCAGAACTCAACAGCATACCCAGCAAAAGCCATTGGTTTCCATTTTGGA GAGCCATTTGAGAACACTTCTTTGAACCAGCAAGTTGTGTCTGCTGAGCAGAAAATATCTCCTGCAGGTT TTCACCATATGCAGCTCAACGGCACCCCGAAGACATTCCTTCCTCCCCAGAAGACAACAAGAGTTGACAC CAGGGAGACTTTCTACAATGAAGACTACTGGATGAGGACACTGAACTCTGACAAGACTCTCTTGGTCCAA AATACAGCCATGTGTGAGAATCAAAGGGTTACCTTTGACAATCATCAAATTGCTCTCTATGGAAGCCATG CAAGCCAGGACCCAAATGTGGGTCACATCTTTGCTTCTGAACATGACTGGACCTCCTACGACAGTCAGAT GACAACCAGGAATGGAGGGCATCTGACTCTCTATGGGAGTCAGATGACAGCACCCAGTTATAGCCAGACC CTTCATCCCAATCAAATCATAACATCCTTTTCTGAACAGAACTATTTTGAGGATCAGCAGAGTAACCTAG CAGTTAATAATGGATTCTATGAAGATCAGGTGATATTGCCAAATGGATACCAGGCTTTCTATGAGCCTCA GATGAGAGCTAACTTTGACCAAACAAATGACCAAATGAAATCCTTCAGTGGTCAGAATGTCTGCAAAAGT CAGGAGAATCTCCTCAGTGGTGAGTGCTCCCTCTCTGGTTATCAGATATCAGGCTACAGATGTGACCAGG ATTTGATTGTGAATCCCCAGGTGCCAAGCCCAATTGGTGGCCAGCCTCTCTCTGACTTTCAGATACAAAC CTCCAGTTTTGATACAACTCTTCCTGGATCTAAAAGGACAAGATCCAGTGCAGAAGACAACCTGGCTTGC CCTTTGGAGACAAGTCCCAGATCTGAAGAGACATTCTACTTGGGTCAAATGAGGACCTCTGTTGATCAAA ATGTCTACCCAAGTCAGAATGGAACACCTAATATTAAAGAAAGCCTTGATCGTCAGGTGACTTCATTTAG TTTGCAAGCTCCATATGTGGGTGAATACTCATATCCTTCACGTTCCCCTTTGATTCAAAAACAACCTCAG AAAAACTCTTCAGCTTCCAGTTTAGTCCAGAGACAGCATCCAGAGATGAAGTTAGACTTCAAGACCCAGA GCCCTGTGTCACAGAAGAACCCTAGTCCTTTGAAGCTCTACTTTTGTACTTATGAGGGTTGTACGAAATT TTACAACAGAGCTTACCACCTCAAAGAACACCAGAAGAAACACACTTGTGTGAGGAAATACAGGTGTGAT GAGCCTGGATGCACATGGTCGTTTTTCCGCCTATGTGATCTCAACAGACATAGAGAAAAGCACAGTGGTG AGAGGCCCTACGCTTGTCCCATGTGCTCCACAAACTATTCCAGACTGGTTTATCTCAAAAAGCATCTGGA GAAGAAACATGCTCATGCACAACCCACCACTGCAACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153096
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153096.2, NP_694736.2
RefSeq Size	5107 bp
RefSeq ORF	1650 bp
Locus ID	234203

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