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Puf60 (NM_133691) Mouse Untagged Clone
CAT＃: MC218836
Puf60 (untagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2, (10ug)
CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2410104I19Rik; 2810454F19Rik; SIAHBP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218836 representing NM_133691
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGCGACTATAGCTCTCCAGGTCAATGGCCAACAAGGAGGAGGGTCGGAGCCAGCAGCAGCGG CTGCAGCGGCGGCGGCGGCAGTGGTGGCAGCAGGAGACAAATGGAAACCTCCACAGGGCACAGAATCCAT CAAGATGGAAAATGGACAAAGCACAGGCACCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAG GCCCTCCAGAAGGCCAAGAAATATGCAATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAACAGACCATCG CGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCAGCTCAACGGCAGCGGGCACTGGCTATCATGTG CCGGGTCTATGTGGGCTCCATCTACTATGAGCTGGGAGAAGACACTATTCGCCAGGCCTTTGCTCCCTTT GGCCCCATCAAGAGCATTGATATGTCCTGGGACTCCGTTACCATGAAGCATAAGGGCTTTGCCTTCGTGG AGTACGAGGTCCCAGAAGCTGCGCAGCTCGCTTTGGAGCAGATGAACTCTGTGATGCTTGGGGGCAGGAA CATCAAGGTGGGAAGACCCAGCAACATTGGACAAGCCCAACCCATCATAGATCAGCTGGCTGAGGAAGCT AGGGCTTTCAACCGAATATATGTGGCCTCTGTACATCAAGACCTGTCTGATGATGACATCAAGAGTGTAT TTGAAGCCTTTGGCAAGATCAAGTCTTGTACGCTGGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTA TGGTTTTATCGAATATGAGAAGGCCCAGTCGTCCCAGGATGCTGTGTCCTCCATGAACCTCTTTGATCTG GGTGGCCAGTACTTGAGGGTGGGCAAGGCCGTCACACCCCCCATGCCCCTGCTAACACCTGCCACGCCTG GAGGTCTCCCGCCTGCTGCTGCTGTGGCCGCAGCTGCAGCCACGGCCAAGATTACAGCTCAGGAAGCAGT GGCTGGAGCAGCAGTGCTGGGTACTTTAGCCACACCAGGACTAGTGTCCCCAGCACTGACACTGGCCCAG CCCTTAGGGGCCCTACCCCAGGCTGTCATGGCTGCCCAGGCCCCTGGAGTCATCACAGGTGTGACACCAG CCCGCCCTCCTATTCCGGTCACCATCCCCTCTGTGGGAGTAGTGAACCCCATTCTCGCCAGCCCGCCAAC GTTGGGTCTGTTGGAGCCCAAGAAGGAGAAGGAAGAGGAGGAGCTGTTTCCTGAGTCAGAACGGCCAGAG ATGTTGAGTGAGCAGGAGCACATGAGCATCTCGGGCAGCAGTGCTCGCCACATGGTCATGCAAAAGCTAC TCAGAAAACAGGAGTCTACAGTGATGGTTCTCCGAAACATGGTGGACCCCAAGGACATCGATGACGACCT GGAGGGAGAGGTGACAGAGGAGTGTGGCAAATTTGGTGCTGTGAACCGGGTCATTATCTATCAAGAAAAA CAGGGCGAAGAGGAAGATGCAGAAATCATCGTCAAGATTTTTGTGGAATTCTCCATGGCCTCAGAGACTC ACAAGGCCATCCAGGCCCTCAATGGGCGCTGGTTTGGTGGTCGCAAGGTGGTGGCTGAAGTGTATGACCA GGAGCGTTTTGATAACAGCGACCTCTCCGCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133691 |
| Insert Size | 1644 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133691.5, NP_598452.2 |
| RefSeq Size | 1878 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 67959 |
| UniProt ID | Q3UEB3 |
| Gene Summary | DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218937 | Puf60 (tGFP-tagged) - Mouse poly-U binding splicing factor 60 (Puf60) transcript variant 2, (10ug) |
CNY 5,990.00 |
|
| MR218937 | Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2 |
CNY 5,420.00 |
|
| MR218937L3 | Lenti ORF clone of Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2 |
CNY 7,320.00 |
|
| MR218937L4 | Lenti ORF clone of Puf60 (mGFP-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2 |
CNY 7,320.00 |
