Eif3d (NM_018749) Mouse Untagged Clone

CAT#: MC218829

Eif3d (untagged) - Mouse eukaryotic translation initiation factor 3, subunit D (Eif3d), (10ug)



  "NM_018749" in other vectors (4)

CNY 7,030.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 66/67kDa; AA407891; eIF3p66; Eif3s7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218829 representing NM_018749
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGTTCATGACACCTGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCCGTTCCTGAGC
AATTTCGGGATATGCCCTACCAGCCATTCAGCAAAGGAGATCGGCTGGGAAAGGTTGCAGACTGGACAGG
GGCCACATACCAGGACAAGAGGTACACAAACAAGTATTCCTCTCAGTTCGGTGGGGGAAGTCAGTATGCA
TATTTCCATGAGGAGGATGAGACAAGCTTCCAGCTGGTGGACACGGCACGGACACAGAAGACCGCCTACC
AGCGGAACCGGATGAGATTCGCACAGCGCAACCTCCGCAGAGACAAAGATCGGAGGAACATGGTGCAGTT
CAACCTACAGACCCTGCCCAAGAGTGCCAAGCAGAAAGAGAGAGAACGAATTCGCTTGCAGAAAAAATTC
CAGAAGCAATTTGGAGTGAGGCAAAAATGGGACCAGAAGTCACAGAAACCCCGAGACTCCTCAGTTGAAG
TTCGCAGTGACTGGGAGGTGAAGGAGGAGATGGACTTCCCTCAGCTGATGAAGATGCGCTACTTGGAAGT
GTCAGAGCCTCAAGACATCGAGTGCTGCGGAGCCCTGGAGTACTACGACAAAGCCTTTGACCGCATCACC
ACAAGGAGTGAGAAGCCCCTGCGGAGCATCAAGCGCATCTTTCACACCGTCACCACCACAGACGACCCTG
TCATCCGGAAGCTGGCAAAAACGCAGGGCAATGTGTTTGCCACTGACGCCATCCTGGCCACGCTGATGAG
CTGCACCCGCTCCGTGTACTCCTGGGACATCGTTGTCCAGAGAGTCGGCTCTAAACTCTTCTTTGACAAG
AGGGACAACTCTGACTTTGACCTCCTGACTGTGAGTGAGACAGCCAATGAGCCGCCTCAAGATGAAGGCA
ACTCCTTCAACTCACCCCGGAACCTGGCCATGGAAGCCACCTACATCAACCACAACTTCTCTCAGCAGTG
CCTGAGAATGGGAAGAGAAAGATACAATTTCCCCAACCCAAACCCATTTGTGGAGGACGACATGGATAAG
AATGAGATCGCCTCCGTCGCTTACCGTTACCGCAGGTGGAAGCTTGGAGATGACATCGACCTTATCGTCC
GCTGTGAACACGATGGTGTCATGACCGGGGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACGCTCAA
TGAATGGGACTCCAGGCATTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGTGGGGCTGTC
ATCGCCACTGAATTGAAGAACAACAGCTACAAGTTGGCACGGTGGACCTGCTGTGCTTTGCTGGCTGGAT
CTGAGTACCTGAAGCTCGGGTATGTGTCCCGGTACCACGTAAAGGACTCCTCACGCCATGTCATCCTGGG
CACCCAGCAGTTTAAGCCCAATGAGTTTGCCAGTCAGATCAACCTGAGCGTGGAGAATGCCTGGGGCATC
CTGCGCTGCGTCATCGACATCTGCATGAAGCTGGAGGAGGGCAAGTACCTCATCCTCAAGGACCCCAACA
AGCAGGTCATCCGGGTCTACAGCCTGCCTGATGGCACCTTCAGCTCTGAGGAGGACGAAGAGGACGAGGA
GGAGGAAGAGGAGGAAGAGGAAGAGGAAGAAACTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_018749
Insert Size 1647 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018749.2, NP_061219.2
RefSeq Size 1910 bp
RefSeq ORF 1647 bp
Locus ID 55944
UniProt ID O70194
Gene Summary mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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