Cdc25b (NM_001111075) Mouse Untagged Clone

CAT#: MC218771

Cdc25b (untagged) - Mouse cell division cycle 25 homolog B (S. pombe) (Cdc25b), transcript variant 2, (10ug)



  "NM_001111075" in other vectors (3)

CNY 7,030.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI604853
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218771 representing NM_001111075
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTACCCCTGCAGAAGTCTGCGCCGGGTTCAGCTCTCAGTCCTGCCCGCGTGCTGGGTGGCATTC
AGCGGCCGCGCCACCTCTCGGTCTTTGAGTTTGAGTCTGATGGCTTCCTGGGGTCTCCGGAGCCTACAGC
TTCCTCCTCTCCGGTTACCACTCTTACACAGACCATGCACAACCTCGCTGGGCTCGGCAGTGAGCCTCCA
AAAGCTCAAGTAGGAAGCCTGTCGTTCCAGAACAGGCTGGCAGACCTATCCCTGTCCAGGCGCACCTCTG
AGTGCTCCCTGTCATCTGAGTCCTCAGAATCTTCGGATGCAGGTCTGTGCATGGACTCCCCCAGCCCTGT
GGACCCGCAGATGGCAGAGCGCACGTTTGAACAGGCCATTCAGGCAGCCAGTCGGGTCATTCAAAATGAG
CAGTTTACCATAAAACGCTTCCGATCCTTACCAGTGAGGCTGCTGGAACACAGTCCGGTGCTGCAGAGCA
TCACCAACTCCCGAGCACTGGACAGCTGGAGGAAAACTGAAGCAGGCTACCGAGCCGCCGCCAATAGTCC
TGGGGAGGACAAAGAGAATGTGCGCTTCCAGAGACCAAGATGGAGGAGCTCCAGGGAGAGAAGGTGTCTT
ACCACTGAGTGGAAGATGGAAGTAGAGGAGCTGAGCCCGGTGGCACAGTCTTCTTCCTTGACTCCTGTCG
AAAGGGCTTCTGAAGAAGATGACGGATTTGTGGACATCCTGGAGAGTGATTTAAAGGATGACGAGAAGGT
CCCCGCGGGCATGGAGAACCTCATTAGTGCCCCACTGGTCAAAAAGCTGGATAAGGAAGAGGAACAGGAT
CTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGCTCCCCATCCATGCCATGCAGTGTGATCCGACCCA
TCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATGTGCCTGTCCAGAGCAAGCGCAGGAAAAGTGTGAC
ACCCCTGGAAGAGCAGCAGCTTGAAGAACCTAAGGCCCGTGTCTTTCGCTCAAAGTCGCTGTGTCATGAG
ATTGAGAACATCCTGGATAGTGACCACCGTGGACTGATCGGAGATTACTCTAAGGCCTTCCTCCTGCAGA
CCGTGGATGGCAAACACCAAGACCTTAAGTACATCTCACCAGAAACTATGGTGGCCCTGTTAACAGGCAA
GTTCAGCAACATCGTGGAGAAATTTGTCATTGTGGACTGCAGATACCCCTATGAGTATGAAGGCGGGCAT
ATCAAGAATGCTGTGAACCTGCCCCTGGAACGGGATGCTGAGACCTTTCTGCTGCAGCGTCCCATCATGC
CTTGTAGCCTGGACAAGAGAATCATCCTCATTTTCCACTGTGAATTCTCGTCTGAGCGTGGACCACGAAT
GTGCCGCTTCATCAGGGAACGGGACCGTGCAGCTAACGACTACCCCAGCCTGTACTACCCGGAGATGTAC
ATCCTCAAAGGCGGCTACAAGGAGTTCTTCCCACAGCATCCGAACTTTTGTGAGCCCCAGGACTACCGAC
CCATGAACCACGAGGCTTTCAGGGATGAGCTGAGGAACTTTCGCCTTAAGACTCGCAGCTGGGCTGGGGA
ACGGAGCAGGAGGGAACTTTGTAGCAGGCTGCAAGACCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001111075
Insert Size 1653 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001111075.4, NP_001104545.1
RefSeq Size 3028 bp
RefSeq ORF 1653 bp
Locus ID 12531
Gene Summary Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame coding exon and uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...