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Rapgef5 (BC046627) Mouse Untagged Clone
CAT＃: MC218582
Rapgef5 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | mmr-gef, mKIAA0277, 4932413M22, GFR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC046627
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCTCCCGCTTGCGGGTCTTTGACCCCCCTCTAGAGAGGAAAGATTCCGCGGCGCTCTCAGAGC GCCAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATCGACGAAGAAGATGAGGACGATGA ATGGAGCAGCCGCTCACAGTCTTCCACGGAGGATGACTCGGTGGACTCTCTGCTCTCTGACAGATATGTG GTGGTGTCTGGTACCCCGGAGAAGATCTTGGAGCACCTTTTGAATGACTTGCACCTGGCAGAGGTTCAGC ACAAAGAAACAGAAACACTCCTTGATGACTTCCTCCTCACGTACACCGTATTCATGACAACTGATGACTT GTGCCAGGCGCTGCTAAGACACTATTCTGCCAAGAAGTATCAAGGGGAAGAAGAAAACTCAGATGTCCCT TGTCGGAAGCGCAAGGTCTTACATCTGGTTTCCCAGTGGATTTCTCTGTACAAGGACTGGCTACATGAAG ATGAGCATTCAAAAATGTTTCTAAAGACCATATACAGGAACGTGCTGGATGATGTCTATGAGTACCCGAT TCTTGAGAAAGAGCTGAAAGAGTTTCAGAAGATACTTGGCGTGTACCGCCGTCACACTGTTGATGAATAC TCACCACAGAAGAAGAATAAAGCCCTTTTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCACAGAG GAACTGTGGCAGAAACAGAGGAAATTTTTTGCCACGTGTATATAACGGAGCACTCCTATATCAGTGTGAA GGCAAAAGTGTCGAGTACAGCCCAGGAGATCCTGAAGGTCGTGGCCGAGAAGCTGCAGCGTGCCAAAGAG GATCTGGCTCTGGTAGCCATTACATTCTCTGGAGAAAAGCATGAATTTCAGCCAAATGACTTGGCCATTT CCAAATCCCTGGAGGCATCTGGTCGGATATATGTCTACCGAAAAGATCTGGCTGACACTTTGAATCCTTT GGCAGAAAATGAGGAATCCCAGCAAAGGTCAATGAGGATTTTGGGGATGAACACGTGGGACCTTGCTCTG GAATTAATGAGTTTTGATTGGAGTCTGTTCAATTCAATCCACGAGCAAGAGCTGATCTACTTCACATTCA GCAGACAGGGGAACGGAGAGAATACTGTGAACCTCAGCCTTCTGCTCCAGAGATGTAACGAGGTCCAGCT CTGGGTAGCCACAGAGATTCTGCTCTGCAGCCAGCTAGGCAAGCGCGTGCAGCTGGTGAAAAAATTCATC AAGATTGCAGCTCACTGCAAAGCCCAACAGAACCTAAACTCTTTCTTTGCCATCGTGATGGGTCTCAACA CCGCCTCTGTCAGCCGGCTATCACAGACCTGGGAGAAAATCCCTGGGAAGTTTAAGAAGCTTTTCTCTGA ACTTGAGAGTTTAACGGATCCTTCCCTAAACCACAAAGCCTACAGGGATGCGTTCAAAAAGATGAAGCCA CCAAAAATCCCTTTCATGCCCTTACTGCTTAAAGATGTAACGTTTATTCATGAAGGAAATAAAACATTCT TGGATAATCTCGTCAATTTTGAAAAGCTGCACATGATTGCAGACACTGTCCGAACCCTGAGACACTGCAG GACTAACCAGTTTGGAAGTGACGTGTCTCCAAAGGAGCAGCAAGAGCTGAAGTCCTATGTCAACCACCTG TATGTCATCGACAGCCAGCAAGCCCTGTTCGAGCTCTCTCACAGGCTGGAGCCTCGAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC046627 |
| Insert Size | 1743 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC046627, AAH46627 |
| RefSeq Size | 2954 bp |
| RefSeq ORF | 1742 bp |
| Locus ID | 217944 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209090 | Rapgef5 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 3,710.00 |
|
| MR209090 | Rapgef5 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 4,320.00 |
|
| MR209090L3 | Lenti ORF clone of Rapgef5 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 5,230.00 |
|
| MR209090L4 | Lenti ORF clone of Rapgef5 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 5 (cDNA clone MGC:54925 IMAGE:6494175) |
CNY 5,230.00 |
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