Bcam (BC004826) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gplu, B-CAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004826 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCCCCTGACGCCCGCGCAGGGCTGCTGTGGCTCACCTTCCTGCTGTCGGGCTACTCAGGTGCCC AGGCTGAGCTGCACGTGTCCGTGCCCCCACGGGTGGAGGTGATGCGAGGCGAGCAAGTCGCCCTGGACTG CACTCCACGGGAGCACCCTGAGCATTACGTGCTGGAATGGTTCCTCGTTGATGGTACTGGGGCTCGACAC CGTCTGGCTTCTGTGGAACCACAGGGCTCAGAGTTCCTGGGCACAGTCCACTCTCTGGGCCGCGTACCCC CATACGAGGTAGACTCTCGTGGGCGCCTGGTGATAGCAAAGGTCCAGGTGGGCGATGGACGGGACTACGT GTGCGTAGTGAAGGCTGGGGCAGCGGGTACCTCAGAGGCCACCTCAAGTGTCCGTGTGTTTGCAACACCA GAGGATACGGAGGTGTCTCCCAACAAAGGGACGCTGTCTGTGATGGACCAGTTTGCCCAGGAGATTGCCA CCTGCAGCAGCAACAACGGAAACCCCGTGCCTCGGATCACATGGTACCGAAACGGGCAGCGTCTGGAAGT ACCCATGGAGGTGAACCAAAAGGGCTACATAACCATCCGTACTGTCCGGGAAGCCTCAGGCCTGTACTCC CTGACCAGCACCCTCTACCTGAGGCTGCACAAGGATGATCGGGATGCCAACTTCCACTGTGCTGCTCACT ACGACTTGCCCTCAGGCCAACATGGCCGCCTGGACAGCCATACCTTTCGCCTCACCCTGCACTATCCCAC GGAACATGTAGAATTCTGGGTGGGGAGCCCGTCCACCACGGAGGGCTGGGTCCGTGAGGGTGATGCTGTC CAGCTGCTCTGCCAGGGCGATGGCAGCCCCAGCCCAGAGTATTCTTTTTTCCGCCAGCAGGGTACCCAGG AGGAACAGCTGAATGTCAACCTCAAGGGAAACTTGACCCTAGAACGGGTGCATCGGAACCAGAGCGGGAT CTACGGCTGCAGAGTGGAGGATTACGATGCCGACGAGGAAGTGCAACTTGTCAAGAAGCTAAAGCTGCAC GTGGCCTACCTGGATCCATTGGAGCTGAGCGTGCCTGAGGAGCTCTTCGTATTCCTGAACAGCAGCAGCA CGGTGGTGAACTGCTCCGCTAGAGGCCTGCCTACTCCTACTGTGCGATGGACCAAGGATTCTGTGACCCT TGCAGACGGCCCCATGCTCTCTCTCCAGTCTGTGACCTTTGACTCAGCTGGCACCTATACCTGTGAGGCG TCCACACCCACAGTCCCCCTCCTGAGCCGCACACAGAGTTTCCAGCTCATCGTCCAAGGAGCACCAGAGT TAAAACCGAATGAGATAATGCCCAAATCAGGGAATAGCTGGACAGAAGGCGATGAAGTCATGCTGACCTG CTCCGCCCGGGGCTTCCCAGAGCCCAAACTCACCTGGAGTCAGCGAGGCGATACTCCAGCAGAGCCGCCC TTTGAGGGTAGAGGCTGGAAGAGCAGCTCGCTGATGGTGAAAGTGACCAGCGCCCTGAGCCGAGAGGGCG TGTCCTGTGAAGCGTCTAACATCCATGGCAAGAAAGGCCATGTCTTCCACTTTGGCTCTGTGGCCCCTCA GACTGCCCAGGCTGGAGTGGCTGTCATGGCTGTAGCTGTCAGCGTCGGTCTTTTGCTACTCGTGGTCGCT GCTTTCTATTGCATGAGACGCAAAGGGCGCCCTGGCTGCTGCCGGCGAGCAGAGAAAGGGGCGCCGCCAG CCAGGGAACCGGAGTTGAGCCACTCTGGGTCAGAGCGGCCAGAACACACGGGCCTTCTCATGGGTGGGCC CTCCTTCCTTCTCCAGCCTCGGCCCTCCCTTCCTTCCTCTGCCTGCTGGGCAGGGACCCACAGTGGCCAG CGGCCTTGGGGAGGGAAAGAGGGAGGTGGGGGCTGGGAGGGGGCCTTCCTCCAGGGAATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	BC004826
Insert Size	1953 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004826, AAH04826
RefSeq Size	2258 bp
RefSeq ORF	1952 bp
Locus ID	57278
Gene Summary	Laminin alpha-5 receptor. May mediate intracellular signaling (By similarity).[UniProtKB/Swiss-Prot Function]

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