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Papss1 (BC066055) Mouse Untagged Clone
CAT＃: MC218279
Papss1 (untagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI325286; Asapk; SK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC066055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAGCAACCAACGTCACCTATCAAGCCCACCATGTCAGCAGGAATAAGAGAGGGCAGGTGGTGG GGACCAGAGGTGGCTTCCGTGGTTGCACAGTTTGGCTGACAGGATTGTCCGGAGCAGGGAAGACGACCGT GAGCATGGCTCTGGAGGAGTACCTGGTGTGCCACGGCATTCCGTGCTACACTTTGGATGGTGACAACATC CGCCAAGGACTCAATAAGAACCTCGGCTTCAGTCCTGAGGACAGAGAAGAGAACGTTCGCCGCATAGCTG AGGTGGCGAAGCTGTTTGCAGATGCTGGCTTAGTGTGTATCACCAGCTTTATATCGCCTTACACACAGGA TCGCAACAACGCAAGGCAGATTCATGAGGGTGCAAGCTTGCCCTTCTTTGAAGTTTTTGTCGATGCTCCT CTGCATGTCTGTGAGCAGAGGGATGTCAAAGGCCTCTACAAGAAGGCGCGGGCAGGGGAGATAAAAGGCT TCACTGGCATCGATTCTGAGTATGAGAAACCCGAGGCCCCGGAGCTGGTGCTGAAAACGGATTCCTGTGA CGTCAACGACTGCGTCCAGCAGGTTGTGGAGCTTCTTCAGGAACGGGACATCGTCCCTGTGGATGCTTCC TATGAAGTGAAAGAGCTATATGTGCCAGAGAATAAACTTCACTTGGCCAAAACTGATGCAGAAGCCTTAC CAGCCCTGAAAATCAATAAAGTGGATATGCAGTGGGTGCAGGTTTTGGCAGAAGGTTGGGCGACTCCTCT GAACGGCTTCATGAGAGAGAGGGAGTACTTGCAGTGCCTCCATTTCGATTGTCTTCTGGATGGAGGCGTC ATCAACTTATCGGTGCCTATAGTTCTGACAGCTACGCACGAGGATAAGGAGAGGCTGGACGGCTGCACCG CGTTCGCTCTGGTGTATGAGGGCCGCCGCGTGGCCATCCTTCGGAATCCTGAATTTTTTGAGCACCGGAA AGAGGAGCGGTGTGCCAGACAGTGGGGAACAACATGCAAGAACCACCCCTACATCAAGATGGTTCTGGAA CAAGGGGATTGGCTGATTGGAGGAGATCTTCAAGTCCTGGACCGGATTTACTGGAATGATGGTCTTGATC AGTACCGCCTTACCCCGACGGAGCTCAAGCAGAAGTTTAAAGATATGAACGCTGATGCTGTCTTTGCATT TCAGTTGCGCAACCCAGTGCACAACGGGCACGCTCTGTTAATGCAGGATACCCACAAGCAGCTTCTGGAG AGGGGCTACCGGCGCCCTGTCCTGCTCCTCCATCCTCTTGGTGGCTGGACGAAGGATGACGATGTCCCTC TGATGTGGCGTATGAAGCAGCACGCTGCAGTGCTGGAGGAGGGCATCCTGGATCCTGAAACGACAGTGGT GGCCATTTTCCCGTCTCCTATGATGTATGCTGGGCCAACCGAGGTCCAGTGGCACTGCAGGGCGCGGATG GTGGCCGGAGCCAATTTTTATATTGTTGGACGAGACCCTGCTGGCATGCCTCATCCGGAGACAGGGAAGG ACCTCTATGAGCCAACACATGGTGCCAAAGTGCTGACGATGGCCCCAGGCCTGATTACCTTGGAAATCGT TCCCTTCCGAGTTGCAGCTTACAACAAGAAAAAGAAGCGGATGGACTACTATGACTCTGAGCACCACGAA GACTTCGAGTTTATTTCAGGAACGAGGATGCGCAAGCTGGCACGCGAAGGCCAGAAACCTCCAGAGGGCT TCATGGCCCCCAAGGCCTGGACTGTGCTGGTAGAGTACTACAAGTCCTTAGAGAAAGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC066055 |
| Insert Size | 1812 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066055, AAH66055 |
| RefSeq Size | 2673 bp |
| RefSeq ORF | 1811 bp |
| Locus ID | 23971 |
| Gene Summary | Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:7493984). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209336 | Papss1 (tGFP-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 3,800.00 |
|
| MR209336 | Papss1 (Myc-DDK-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 4,488.00 |
|
| MR209336L3 | Lenti ORF clone of Papss1 (Myc-DDK-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 5,320.00 |
|
| MR209336L4 | Lenti ORF clone of Papss1 (mGFP-tagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756) |
CNY 5,320.00 |
