Src (NM_009271) Mouse Untagged Clone

CAT#: MC218246

Src (untagged) - Mouse Rous sarcoma oncogene (Src), transcript variant 1, (10ug)



  "NM_009271" in other vectors (4)

CNY 6,940.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW259666; pp60c-src
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218246 representing NM_009271
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGCAACAAGAGCAAGCCCAAGGACGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCTCGGAAAACG
TGCACGGGGCAGGGGGCGCCTTCCCGGCCTCACAGACACCGAGCAAGCCCGCCTCCGCCGACGGCCACCG
CGGGCCCAGCGCCGCCTTCGTGCCGCCCGCGGCCGAGCCCAAGCTCTTCGGAGGCTTCAACTCCTCGGAC
ACCGTCACCTCCCCGCAGAGGGCGGGGCCTCTGGCAGGTGGGGTGACCACCTTTGTGGCCCTCTATGACT
ATGAGTCACGGACAGAGACTGACCTGTCCTTCAAGAAAGGGGAGCGGCTGCAGATTGTCAATAACACGAG
GAAGGTGGATGTCAGAGAGGGAGACTGGTGGCTGGCACACTCGCTGAGCACGGGACAGACCGGTTACATC
CCCAGCAACTATGTGGCGCCCTCCGACTCCATCCAGGCTGAGGAGTGGTACTTTGGCAAGATCACTAGAC
GGGAATCAGAGCGGCTGCTGCTCAACGCCGAGAACCCGAGAGGGACCTTCCTCGTGAGGGAGAGTGAGAC
CACAAAAGGTGCCTACTGCCTCTCTGTATCCGACTTCGACAATGCCAAGGGCCTAAATGTGAAACACTAC
AAGATCCGCAAGCTGGACAGCGGCGGTTTCTACATCACCTCCCGCACCCAGTTCAACAGCCTGCAGCAGC
TCGTGGCTTACTACTCCAAACATGCTGATGGCCTGTGTCACCGCCTCACTACCGTATGTCCCACATCCAA
GCCTCAGACCCAGGGATTGGCCAAGGATGCGTGGGAGATCCCCCGGGAGTCCCTGCGGCTGGAGGTCAAG
CTGGGCCAGGGTTGCTTCGGAGAGGTGTGGATGGGGACCTGGAACGGCACCACGAGGGTTGCCATCAAAA
CTCTGAAGCCAGGCACCATGTCCCCAGAGGCCTTCCTGCAGGAGGCCCAAGTCATGAAGAAACTGAGGCA
CGAGAAACTGGTGCAGCTGTATGCTGTGGTGTCGGAAGAACCCATTTACATTGTGACAGAGTACATGAAC
AAGGGGAGTCTGCTGGACTTTCTCAAGGGGGAAACGGGCAAATATTTGCGGCTACCCCAGCTGGTGGACA
TGTCTGCTCAGATCGCTTCAGGCATGGCCTATGTGGAGCGGATGAACTATGTGCACCGGGACCTTCGAGC
CGCCAATATCCTAGTAGGGGAGAACCTGGTGTGCAAAGTGGCCGACTTTGGGTTGGCCCGGCTCATAGAA
GACAACGAATACACAGCCCGGCAAGGTGCCAAATTCCCCATCAAGTGGACCGCCCCTGAAGCTGCTCTGT
ACGGCAGGTTCACCATCAAGTCGGATGTGTGGTCCTTTGGGATTCTGCTGACCGAGCTCACCACTAAGGG
AAGAGTGCCCTATCCTGGGATGGTGAACCGTGAGGTTCTGGACCAGGTGGAGCGGGGCTACCGGATGCCT
TGTCCCCCCGAGTGCCCCGAGTCCCTGCATGACCTTATGTGCCAGTGCTGGCGGAAGGAGCCCGAGGAGC
GGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAAGACTACTTTACGTCCACTGAGCCACAGTACCAGCC
CGGGGAGAACCTATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009271
Insert Size 1626 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009271.3, NP_033297.2
RefSeq Size 3887 bp
RefSeq ORF 1626 bp
Locus ID 20779
UniProt ID P05480
Gene Summary Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN) (Probable). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN) (By similarity). In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors (PubMed:9344858). Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1. Plays a role in EGF-mediated calcium-activated chloride channel activation (By similarity). Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a critical role in the stimulation of the CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor (Probable). Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus (By similarity). Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function (PubMed:14739300). Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase (PubMed:12615910). Phosphorylates DDR2 on tyrosine residues, thereby promoting its subsequent autophosphorylation. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-738'. Enhances DDX58/RIG-I-elicited antiviral signaling. Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373' and 'Tyr-376'. Phosphorylates BCAR1 at 'Tyr-226'. Phosphorylates CBLC
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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