United States
Germany
Japan
United Kingdom
China
Nefl (NM_010910) Mouse Untagged Clone
CAT＃: MC218208
Nefl (untagged) - Mouse neurofilament, light polypeptide (Nefl), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI847934; CMT2E; NF-L; NF68; Nfl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218208 representing NM_010910
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCGTTCGGCTACGATCCGTACTTTTCGACCTCCTACAAGCGGCGCTATGTGGAGACGCCCCGGG TGCACATCTCCAGCGTGCGCAGCGGCTACAGCACGGCGCGCTCCGCGTACTCCAGCTACTCCGCGCCGGT CTCCTCCTCGCTGTCCGTGCGCCGCAGCTACTCGTCCAGCTCTGGCTCTTTGATGCCCAGCCTGGAGAAT CTCGATCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCTATCCGCACACAAGAGAAGGCACAGC TGCAGGACCTCAACGATCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAACAAGGT CCTGGAAGCCGAGCTGTTGGTGCTGCGCCAGAAACACTCTGAGCCTTCCCGCTTCCGCGCCCTGTACGAG CAGGAGATCCGCGATCTGCGGCTGGCAGCGGAAGACGCCACTAACGAGAAGCAGGCGCTGCAGGGCGAGC GCGAGGGGCTGGAGGAGACTCTGCGCAACCTGCAGGCTCGCTATGAGGAAGAAGTGCTGAGCCGCGAGGA CGCCGAGGGCCGGCTGATGGAAGCGCGCAAAGGTGCGGATGAGGCCGCGCTCGCCCGCGCCGAGCTGGAG AAGCGCATCGACAGCCTGATGGACGAGATAGCTTTCCTGAAGAAGGTGCACGAGGAAGAGATCGCCGAGC TGCAGGCTCAGATCCAGTATGCTCAGATCTCCGTGGAGATGGACGTGTCCTCCAAGCCCGACCTCTCCGC CGCTCTCAAGGACATCCGCGCTCAGTACGAGAAGCTGGCCGCCAAGAACATGCAGAACGCCGAAGAGTGG TTCAAGAGCCGCTTCACCGTGCTAACCGAGAGCGCCGCCAAGAACACCGACGCTGTGCGCGCTGCCAAGG ACGAGGTGTCGGAAAGCCGCCGCCTGCTCAAGGCTAAGACCCTGGAGATCGAAGCCTGCCGGGGTATGAA CGAAGCTCTGGAGAAGCAGCTGCAGGAGCTAGAGGACAAGCAGAATGCAGACATTAGCGCCATGCAGGAC ACAATCAACAAACTGGAGAATGAGCTGAGAAGCACGAAGAGCGAGATGGCCAGGTACCTGAAGGAGTACC AGGACCTCCTCAATGTCAAGATGGCCTTGGACATCGAGATTGCAGCTTACAGAAAACTCTTGGAAGGCGA AGAGACCAGGCTCAGTTTCACCAGCGTGGGTAGCATAACCAGCGGCTACTCTCAGAGCTCGCAGGTCTTC GGCCGTTCTGCTTACAGTGGCTTGCAGAGCAGCTCCTACTTGATGTCTGCTCGCTCTTTCCCAGCCTACT ATACCAGCCACGTCCAGGAAGAGCAGACAGAGGTCGAGGAGACCATTGAGGCTACGAAAGCTGAGGAGGC CAAGGATGAGCCCCCCTCTGAAGGAGAAGCAGAAGAGGAGGAGAAGGAGAAAGAGGAGGGAGAGGAAGAG GAAGGCGCTGAGGAGGAAGAAGCTGCCAAGGATGAGTCTGAAGACACAAAAGAAGAAGAAGAAGGTGGTG AGGGTGAGGAGGAAGACACCAAAGAATCTGAAGAGGAAGAGAAGAAAGAGGAGAGTGCTGGAGAGGAGCA GGTGGCTAAGAAGAAAGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010910 |
| Insert Size | 1632 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010910.1, NP_035040.1 |
| RefSeq Size | 2014 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 18039 |
| UniProt ID | P08551 |
| Gene Summary | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224212 | Nefl (tGFP-tagged) - Mouse neurofilament light polypeptide (Nefl), (10ug) |
CNY 5,990.00 |
|
| MR224212 | Nefl (Myc-DDK-tagged) - Mouse neurofilament, light polypeptide (Nefl) |
CNY 6,464.00 |
|
| MR224212L3 | Lenti ORF clone of Nefl (Myc-DDK-tagged) - Mouse neurofilament, light polypeptide (Nefl) |
CNY 7,320.00 |
|
| MR224212L4 | Lenti ORF clone of Nefl (mGFP-tagged) - Mouse neurofilament, light polypeptide (Nefl) |
CNY 7,320.00 |
