Chka (BC060218) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Chetk-alpha, ChoK, EtnK-alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC060218 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAACCAAGTTCTGCACCGGGGGCGAGGCCGAGCCGTCGCCTCTCGGGCTGCTGCTGAGCTGCGGTG GCAACGCTGCCCCGACGCCCGGCGTAGGGCAGCAGCGGGACGCCGCAGGCGAGCTGGAGTCCAAGCAGCT TGGTGGCCGAACCCAACCTCTCGCGCTGCCGCCGCCGCCGCCGCCGCCCCTGCCGCTGCCCCCGCCGCCA TCACCGCCGCTAGCGGACGAACAACCCGAGCCCCGGACCAGGCGCAGGGCCTACCTGTGGTGTAAGGAAT TCCTGCCCGGAGCCTGGCGAGGCCTTCGCGAGGACCAGTTCCACATCAGTGTCATCAGGGGTGGTCTCAG TAACATGCTGTTCCAGTGCTCCCTGCCAGACTCCATAGCCAGCGTTGGCGATGAGCCTCGGAAAGTGCTC TTGCGGCTCTATGGGGCAATCTTAAAGATGGGGGCTGAGGCGATGGTTCTGGAGAGTGTTATGTTTGCCA TTCTTGCAGAGAGGTCACTTGGGCCAAAACTCTTTGGCATCTTTCCCCAAGGCCGACTGGAGCAGTTTAT CCCGAGCCGGCGATTGGACACTGAAGAGTTACGTTTACCAGATATTTCTGCAGAAATAGCTGAGAAAATG GCCACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGAACAATGGAAAAAT ACCTGAATCAAGTACTAAGACTCAAATTCAGCAGGGAGGCCAGAGTTCAGCAACTGCACAAGATCCTCTC TTACAACCTGCCTCTTGAGCTCGAGAACCTGAGGTCATTGCTGCAGTATACTAGATCTCCAGTTGTATTT TGTCATAATGACTGTCAAGAAGGTAATATCTTATTGTTGGAAGGCCAGGAGAACTCTGAAAGGCGGAAGC TGATGCTTATTGACTTTGAGTACAGCAGTTACAATTACAGGGGATTTGACATTGGAAATCATTTCTGTGA ATGGATGTATGATTATACCTATGAGAAGTATCCTTTCTTCAGAGCGAACATTCAGAAGTATCCTTCCCGA AAACAACAGGTAGGAGAAGCATTTGTATTTATAAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	BC060218
Insert Size	4050 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060218
RefSeq Size	4685 bp
RefSeq ORF	1088 bp
Locus ID	12660
Gene Summary	Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline (By similarity).[UniProtKB/Swiss-Prot Function]

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