Polr2m (NM_178602) Mouse Untagged Clone

CAT#: MC218078

Polr2m (untagged) - Mouse glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a), transcript variant 1, (10ug)



  "NM_178602" in other vectors (7)

CNY 6,940.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA407243; AA408799; AI303746; AW553238; D9Wsu138e; Grinl1a; mKIAA4185
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218078 representing NM_178602
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTCGCTGCCCCGCGGCTTCGAGCCCCCAGCTCCCGAGGACTTGGGGCGGCAGAGTTCGGCGGAGC
TGCGGGAGAGGTTGAGGCGCCAGGAGAGACTTTTGCGCAACGAAAAATTCATTTGCAAATTGCCCGACAA
AGGTAAAAAGATCTCAGACACAGTTGCCAAACTGAAAGCTGCCATTTCAGAACGTGAAGAGGTTAGAGGG
AGAAGTGAACTGTTTCATCCTGTTAGTGTAGACTGTAAGCTAAGGCAAAAAGCAACCACAAGAGCTGACA
CCGATGTAGACAAGGCCCAGAGTTCTGACCTGATGCTTGATACTTCATCATTAGATCCTGACTGTTCCTC
AATAGACATTAAGTCATCTAAATCAACCTCAGAAACACAGGGACCTACACATCTCACTCACAGAGGCAAT
GAAGAGACTTTGGAGGCTGGCTACACAGTAAACAGCAGCCCAGCTGCCCACATCCGAGCCCGGGCGCCCT
CATCCGAAGTTAAGGAGCATCTCCCCCAGCACTCTGTTTCAAGTCAAGAGGAAGAGATCTCCAGCAGCAT
CGACAGTCTCTTCATCACTAAATTGCAAAAAATCACAATTGCAGACCAGAGTGAACCCTCAGAAGAAAAC
ACCAGCACTGAGAACTTTCCAGAACTGCAGAGTGAGACTCCTAAGAAGCCTCATTACATGAAAGTGCTAG
AAATGCGAGCCAGAAACCCAGTGCCCCCTCCTCATAAGTTTAAGACCAATGTGTTACCCACACAACAGAG
TGACTCACCAAGTCATTGTCAGAGGGGCCAGTCTCCTGCTTCCTCAGAAGAGCAGCGACGAAGGGCTAGG
CAGCATCTTGATGATATCACAGCAGCGCGCCTCCTTCCGCTCCACCACCTGCCTGCACAGCTGCTTTCCA
TAGAAGAGTCGCTGGCCCTGCAGAGGGAGCAGAAGCAGAATTATGAGGAGATGCAGGCAAAGCTCGCAGC
ACAGAAACTGGCCGAGAGACTGAATATTAAAATGCAGAGCTACAATCCAGAAGGGGAGTCTTCAGGGAGA
TACCGAGAAGTGAGGGACGAAGCTGATGCCCAGTCCTCGGATGAGTGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_178602
Insert Size 1101 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_178602.3, NP_848717.1
RefSeq Size 2265 bp
RefSeq ORF 1101 bp
Locus ID 28015
UniProt ID Q6P6I6
Gene Summary Isoform 1 appears to be stable component of the Pol II(G) complex form of RNA polymerase II. Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in Mediator-dependent regulation of transcription activation. Isoform 1 acts in vitro as negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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