March2 (BC002144) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC7259
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC002144 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACAGGTGACTGTTGCCACCTCCCTGGCTCCCTATGTGACTGTTCGAGCAGCCCTGCCTTTTCCA AGGTTGTAGAGGCCACAGGGCTTGGGCCACCTCAATATGTAGCACAAGTGACTTCACGGGATGGCCGGCT GCTCTCAACTGTCATCCGGGCTTTGGATTCACAGAGTGACTGTCCCTTCTGCCGAATCTGCCACGAGGGA GCAAATGGGGAGAACTTGCTGTCCCCATGTGGCTGCACGGGCACACTGGGAGCTGTGCACAAGAGCTGCC TGGAGAAATGGCTGTCTTCCTCCAACACCAGCTACTGTGAGCTGTGTCACACTGAGTTTGCAGTGGAAAA GCGGCCCCGACCTCTCACAGAGTGGCTAAAGGACCCAGGGCCGCGCACTGAGAAGCGAACACTGTGCTGT GACATGGTGTGCTTTGTGTTCATCACACCACTGGCTGCCATCTCAGGCTGGCTTTGCCTGCGAGGGGCCC AGGACCACCTCCGTCTGCATAGCCGGCTGGAAGCTGTAGGTCTCATTGCCCTCACCATCGCCCTCTTTAC CATCTATGTGCTCTGGACACTGGTCTCTTTCCGATACCATTGCCAGCTGTACTCGGAATGGAGGAAGACA AATCAGAAAGTCCGCCTGAAGATTCGGGAAGCAGATGGCTCCGAGGATCCACATCACTCCTTGCTGGCTA CTGGACTCTTAAAAAAGGTGGCAGAGGAGACCCCTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	BC002144
Insert Size	741 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002144, AAH02144
RefSeq Size	1376 bp
RefSeq ORF	740 bp
Locus ID	224703
Gene Summary	E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.[UniProtKB/Swiss-Prot Function]

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