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Trim23 (BC059017) Mouse Untagged Clone
CAT＃: MC217929
Trim23 (untagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6330516O20Rik; AI450195; Arfd1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC059017
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCTCGCTGTAAACAAGCCCGGAGCCGGAGTAGACAGTGGGCGGCAGGGCAGCCGGGGGACGG CTGTGGTGAAGGTTCTGGAGTGTGGGGTTTGTGAAGATGTCTTCTCTTTGCAAGGAGATAAAGTTCCTCG CCTCCTCCTCTGTGGCCACACAGTCTGTCATGACTGTCTTACTCGCTTGCCTCTGCATGGAAGAGCAATA CGTTGCCCATTTGATCGACAAGTAACAGACCTAGGAGACTCAGGTGTATGGGGTTTGAAAAAGAACTTTG CTTTACTGGAGCTTTTAGAACGTTTGCAAAATGGGCACATTGGTCAGTATGGAGCTGCAGAAGAGGCCAT TGGGACATCTGGAGAGAGCATCATTCGCTGTGATGAAGATGAAGCTCACGTTGCATCTGTATATTGCACT GTGTGTGCAACTCACTTGTGCTCAGACTGTTCTCAAGTTACTCATTCTACAAAGACATTAGCAAAGCATA GGCGTGTGCCTCTAGCTGATAAACCTCATGAGAAAACCATGTGCTGTCAGCACCAGGTGCATGCCATTGA GTTTGTTTGCTTGGAAGAAGGTTGTCAAACTAGTCCACTCATGTGCTGTGTCTGCAAAGAATATGGAAAG CACCAAGGTCACAAGCATTCAGTACTGGAACCAGAAGCTAATCAGATCCGAGCATCAATTTTAGACATGG CTCACTGCATACGGACCTTCACTGAGGAAATCTCAGATTATTCCAGAAAATTAGTTGGGATTGTTCAGCA CATTGAAGGAGGAGAACAAATAGTAGAAGATGGAATTGGAATGGCTCACACAGAACATGTCCCAGGTACT GCAGAGAATGCCCGATCATGTGTCAGAGCTTATTTTTCTGATTTACATGAAACTCTTTGTCGTCAAGAAG AAATGGCTCTAAGTGTTGTTGATGCCCATGTTCGAGAAAAACTGATTTGGCTTAGGCAGCAACAAGAGGA TATGACTATTCTCTTGTCCCAGGTCTCAACAGCCTGTCTCCACTGTGAAAAAACTCTGCAGCAGGATGAT TGCAGAGTTGTCTTGGCAAAACAAGAAATCACAAGATTACTAGAAACACTGCAGAAACAGCAGCAGCAGT TTACAGAGGTTGCAGATCATATTCAGTTGGATGCCAGTATTCCAGTCACTTTTACAAAGGACAATAGAGT TCATATTGGACCCAAAATGGAAATCCGAGTAGTCACATTAGGATTAGATGGTGCTGGAAAAACTACCATT TTGTTCAAGTTAAAACAAGATGAATTTATGCAACCTATTCCAACAATTGGTTTTAATGTGGAAACTGTGG AATACAAAAATCTAAAATTCACCATTTGGGATGTGGGTGGAAAACACAAATTAAGACCATTGTGGAAACA TTATTACCTCAATACACAAGCTGTTGTATTTGTTGTTGATAGTAGTCATAGAGACAGAATTAGTGAAGCA CACAGTGAACTTGCAAAGTTGTTAACGGAAAAAGAACTCCGAGATGCCTTACTCTTGATTTTTGCTAACA AACAGGATGTAGCTGGAGCACTTTCAGTAGAAGAAATCACTGAACTTCTCAGTCTCCATAAACTATGCTG TGGAAGGAGTTGGTATATTCAGGGCTGTGATGCTCGAAGTGGAATGGGGCTCTACGAAGGGTTGGACTGG CTGTCCCGGCAACTTGTGGCTGCTGGAGTGTTGGATGTTGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC059017 |
| Insert Size | 1725 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059017, AAH59017 |
| RefSeq Size | 3385 bp |
| RefSeq ORF | 1724 bp |
| Locus ID | 81003 |
| Gene Summary | Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209027 | Trim23 (tGFP-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) |
CNY 3,520.00 |
|
| MR209027 | Trim23 (Myc-DDK-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) |
CNY 4,272.00 |
|
| MR209027L3 | Lenti ORF clone of Trim23 (Myc-DDK-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) |
CNY 5,130.00 |
|
| MR209027L4 | Lenti ORF clone of Trim23 (mGFP-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) |
CNY 5,130.00 |
