United States
Germany
Japan
United Kingdom
China
Mier1 (NM_027696) Mouse Untagged Clone
CAT＃: MC217471
Mier1 (untagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 1, (10ug)
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4933425I22Rik; 5830411K19Rik; er1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217471 representing NM_027696
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTATGTTTAATTGGTTTACAGACTGTCTCTGGATTCTTTTCCTGTCAAATTACAAGCCATCTGTTG AGTCTTCAAGTCCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATCCATCAGCTGACATGCTGGT TCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGGAGAGACAAACTTCAGTTCT GAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTCAGCCTCTATGGTTATGACA GCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAGGTGAAGATGATGAAGATGC TGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATATAAAGGATTCATCGGGTCAG GAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCCCAAGATGCTCAGGAAATAA TCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAGAATCTGAAGAAGATGAAGA TTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTTTCAAGCTGAGATTCCAGTT GGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAGCTTCTGTGGGATCCAGAGT GTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGACAGGGGATGAGAAAGGTGT GGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATATGAGTTGGTTAAATGCAGC TTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCTCGAGAGGAGTTATCTGTGT GGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAAAAGATTTTCATCTGATTCA GGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTACATGTGGAAAAAGTCTGAG CGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAATCTTCATCCTGGTGTAACGG ATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAGCACCATCCCCCCCTCCCAC TGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAGCAGTAGGAATCAGAACGGT GTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTTGAAGGGTTACATGTTAATG GACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATGGTTATGAAGCAAATAACCT GACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGATGAAAAAAATGAGAGA CCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCATCTGAGTTTTTCCAGGAAG CAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027696 |
| Insert Size | 1587 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027696.3, NP_081972.2 |
| RefSeq Size | 4838 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 71148 |
| UniProt ID | Q5UAK0 |
| Gene Summary | Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR, initiates translation at an alternate start codon and lacks an alternate exon in the 5' coding region, compared to variant 4. Variants 1 and 3 encode the same isoform (a), which is shorter and has a distinct N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218535 | Mier1 (tGFP-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1) transcript variant 1, (10ug) |
CNY 5,800.00 |
|
| MR218535 | Mier1 (Myc-DDK-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 1 |
CNY 5,230.00 |
|
| MR218535L3 | Lenti ORF clone of Mier1 (Myc-DDK-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 1 |
CNY 7,130.00 |
|
| MR218535L4 | Lenti ORF clone of Mier1 (mGFP-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 1 |
CNY 7,130.00 |
