Insm1 (NM_016889) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	IA-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC217453 representing NM_016889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGGGTTTCTGGTGAAGCGCAGCAAGAAGTCCACGCCCGTGTCCTACCGGGTCCGCGGCGGCG AGGACAGTGACCGGGCGCTGCTGCTGTCACCCGGCTGCGGGGGCGCCCGCGCCGAGCCCCCGGTGCCCAG CCCCGGGCCGCTGCCGCCACCTCCGCCGCCGGCGCTCGCGGAGCGCGCCCATGCTGCGCTCGCCGCCGCG CTCGCCTGCGCGCCAGGCCCGCCGCCGCCACCCCCGCCAGGCCCGCGGGCCGCGCACTTCGGCAACCCCG AGGCTGCGCACCCGGCGCCTCTCTACAGTCCCACGCGGCCGGTGAGCCGCGAGCACGAGAAGCACAAGTA CTTCGAGCGCAGCTTCAACCTGGGCTCGCCGGTGTCCGCTGAGTCCTTCCCCACGCCCGCCGCGCTGCTC GCAGGGGGAGGCAGCGGCGCCAACGGCGCTGGCGGCGGCGGCGGCGGCACCTGCGGCGGAGACGCGCTGC TCTTCGCTCCCGCCGAGCTCAAGATGGGCACTGCGTTCTCCGCCGGCGCCGAGGCGGCCCGGGGTCCTGG GACCGGTCCTCCACTGTCCCCCGCCGCGGCCCTGCGGCCCCCGGGCAAGCGACCGGCGCCCCCCGCCGCT GTCGCTACAGAGCCGCCCGCCAAGGCAGCCAAGGCCCCGAGCGCCAAAAAGCCGAAGGCCATCCGCAAGC TGCACTTCGAGGACGAGGTGACCACGTCGCCGGTTCTGGGGCTCAAGATCAAGGAGGGCCCGGTGGAGGC GCCGCGGGGTCGCGCGGGGGGCGCGACCCGACCCCTGGGCGAGTTCATCTGCCAGCTGTGCAAGGAGGAG TACGCTGACCCGTTCGCGCTGGCGCAGCACAAGTGCTCGCGCATCGTGCGCGTGGAGTACCGCTGCCCAG AGTGCGCCAAGGTCTTCAGCTGCCCGGCCAACCTGGCCTCGCACCGCCGCTGGCACAAACCACGGCCGGT GCCCGCGGCGGCCCGCGCGCCAGAGCCAGAAGCCGCCACCAGGGCGGAGGCGCGCGAGGCTGCGGGCGGC GGCAGCAGCGATCGGGACACGCCGAGCCCTGGCGGCGTATCCGAGTCAGGCTCCGAGGACGGGCTCTACG AGTGCCACCACTGCGCCAAGAAGTTCCGTCGCCAGGCCTATCTGCGCAAGCACCTGCTGGCACATCACCA GGCGCTGCAGGCCAAAGGCGCGCCCCCGCCCCCGCCGCCGCCGCCACCCCCCGCCGAGGACATCCTGGCT TTCTACGCGGGGCCCGACGAAAAGGCGCCCCAGGAGGCCTCGGGCGACGGCGAGGCGGCCGGCGTGCTGG GCCTGAGTGCGACCGCCCAGTGCCACCTGTGCCCAGTGTGCGGGGAGACCTTCCCCAGCAAGGGCGCCCA GGAGCGCCACCTGCGCCTGCTGCACGCTGCCCAGGTGTTCCCCTGCAAGTACTGCCCGGCCACCTTCTAC AGCTCCCCGGGCCTGACCCGGCACATCAACAAGTGCCACCCGTCTGAGAATAGACAGGTGATCCTCCTTC AGGTGCCTGTGCGTCCGGCCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_016889
Insert Size	1566 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016889.3, NP_058585.2
RefSeq Size	3038 bp
RefSeq ORF	1566 bp
Locus ID	53626
UniProt ID	Q63ZV0
Gene Summary	Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-[TG][TC][TC][TT][GA]GGG[CG]A-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (By similarity). Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.