Sgk1 (NM_001161845) Mouse Untagged Clone

CAT#: MC217438

Sgk1 (untagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 1, (10ug)



  "NM_001161845" in other vectors (4)

CNY 6,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Sg; Sgk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217438 representing NM_001161845
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAAACAAAGACATGAATGGATTCCCGGTCAAGAAATGCTCAGCGTTCCAATTTTTTAAGAAACGGG
TACGAAGATGGATCAAGAGCCCCATGGTCAGCGTGGACAAGCATCAGAGCCCCAACTTGAAGTACACTGG
CCCTGCTGGGGTGCATCTTCCCCCTGGGGAGTCAGACTTTGAGGCCATGTGTCAATCATGCCTGGGTGAC
CATGCTTTCCAAAGGGGGATGCTCCCTCCAGAGGAGTCCTGTTCCTGGGAGATCCAACCTGGGTGTGAAG
TGAAAGAACAATGTAATCATGCCAACATCCTGACCAAGCCGGACCCAAGAACCTTCTGGACTAATGATGA
TGCAGCTTTTATGAAACAGAGAAGGATGGGCCTGAACGATTTTATTCAGAAGATTGCCAGCAACACCTAT
GCATGCAAACACGCTGAAGTTCAGTCCATTTTGAAAATGTCCCATCCTCAGGAGCCGGAGCTTATGAACG
CTAACCCCTCTCCTCCGCCAAGTCCCTCTCAACAAATCAACCTGGGTCCGTCCTCCAACCCTCACGCCAA
ACCCTCCGACTTTCACTTCTTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTGGCTAGGCAC
AAGGCAGAAGAAGTATTCTATGCAGTCAAAGTTTTACAGAAGAAAGCCATCCTGAAGAAGAAAGAGGAGA
AGCATATTATGTCAGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTT
CTCATTCCAGACCGCTGACAAGCTCTACTTTGTCCTGGACTACATTAATGGTGGAGAGCTGTTCTACCAT
CTCCAGAGGGAGCGCTGCTTCCTGGAACCACGGGCTCGATTCTACGCAGCTGAAATAGCCAGTGCCCTGG
GCTATCTGCACTCCCTAAACATCGTTTATAGAGACTTAAAACCTGAGAATATTCTCCTAGACTCCCAGGG
GCACATCGTCCTCACTGACTTTGGGCTCTGCAAAGAGAATATTGAGCATAACGGGACAACATCTACCTTC
TGTGGCACGCCTGAGTATCTGGCTCCTGAGGTCCTCCATAAGCAGCCGTATGACCGGACGGTGGACTGGT
GGTGTCTTGGGGCTGTCCTGTATGAGATGCTCTACGGCCTGCCCCCGTTTTATAGCCGGAACACGGCTGA
GATGTACGACAATATTCTGAACAAGCCTCTCCAGTTGAAACCAAATATTACAAACTCGGCAAGGCACCTC
CTGGAAGGCCTCCTGCAGAAGGACCGGACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGA
GTCATATTTTCTTCTCTTTAATTAACTGGGATGATCTCATCAATAAGAAGATTACACCCCCATTTAACCC
AAATGTGAGTGGGCCCAGTGACCTTCGGCACTTCGATCCCGAGTTTACCGAGGAGCCGGTCCCCAGCTCC
ATCGGCAGGTCCCCTGACAGCATCCTTGTCACGGCCAGTGTGAAGGAAGCAGCAGAAGCCTTCCTCGGCT
TCTCCTATGCACCTCCTGTGGATTCCTTCCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001161845
Insert Size 1575 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001161845.2, NP_001155317.2
RefSeq Size 3121 bp
RefSeq ORF 1575 bp
Locus ID 20393
UniProt ID Q9WVC6
Gene Summary This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq, Sep 2009]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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