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Kpna3 (NM_008466) Mouse Untagged Clone
CAT＃: MC217424
Kpna3 (untagged) - Mouse karyopherin (importin) alpha 3 (Kpna3), (10ug)
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | IPOA4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217424 representing NM_008466
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGAACCCCGGCTTGGAGAACCACCGCATCAAGAGCTTCAAGAACAAGGGCCGCGATGTGGAGA CGATGCGAAGACACAGAAATGAAGTGACAGTTGAACTCCGGAAGAACAAAAGAGACGAACACTTACTGAA AAAGAGAAATGTTCCCCAAGAAGAAAGCTTAGAAGATTCAGATGTTGATGCTGATTTTAAAGCACAAAAT GTAACACTAGAAGCTATATTACAGAATGCCACAAGTGATAATCCTGTAGTCCAGTTGAGTGCTGTGCAGG CAGCAAGGAAACTATTATCCAGTGACAGAAATCCACCAATTGATGACCTAATAAAATCTGGGATTTTACC AATTCTAGTCAAATGTCTAGAAAGAGATGATAATCCTTCATTACAGTTTGAAGCTGCTTGGGCACTAACT AATATAGCATCGGGAACTTCTGCACAGACTCAAGCTGTTGTACAGTCTAATGCAGTACCACTTTTTCTGC GACTCCTCCATTCCCCACATCAGAATGTTTGTGAACAAGCGGTGTGGGCTTTGGGAAACATTATAGGTGA TGGTCCTCAATGTAGAGATTATGTCATATCACTGGGAGTTGTCAAACCTCTTCTGTCCTTCATCAATCCC TCCATTCCCATCACCTTCCTTCGGAACGTCACATGGGTCATTGTCAATCTCTGCAGGAATAAAGACCCCC CACCGCCTATGGAGACAGTTCAGGAGATTTTGCCAGCTTTGTGTGTGCTCATCTACCATACGGACATAAA TATTCTTGTAGACACTGTTTGGGCGCTGTCATACTTGACAGACGGGGGAAATGAGCAAATACAGATGGTT ATTGATTCAGGGGTTGTACCCTTCCTCGTACCCCTTCTGAGCCACCAGGAAGTCAAAGTTCAAACTGCAG CACTCAGAGCGGTTGGGAACATAGTGACTGGCACTGATGAGCAGACCCAGGTGGTTCTCAACTGTGATGT CCTGTCCCACTTCCCAAACCTCTTGTCACACCCAAAAGAGAAAATAAATAAGGAAGCAGTATGGTTCCTT TCCAATATAACAGCAGGCAATCAGCAGCAAGTTCAAGCTGTAATAGATGCTGGGTTAATCCCTATGATTA TTCATCAGCTCGCTAAGGGGGACTTTGGAACACAAAAGGAAGCTGCTTGGGCAATTAGCAACTTAACGAT AAGTGGCAGAAAAGATCAAGTTGAATACCTTGTACAGCAGAACGTAATACCACCATTCTGTAACTTACTG TCAGTAAAAGATTCTCAAGTGGTGCAGGTGGTTCTAGATGGTCTTAAAAACATCCTGATAATGGCTGGTG ATGAAGCAAGCACAATAGCTGAGATCATAGAAGAGTGTGGAGGTTTGGAGAAAATTGAAGTTTTGCAGCA ACATGAAAATGAAGACATATATAAATTAGCATTTGAAATCATAGATCAATATTTCTCTGGTGATGATATT GATGAAGATCCTAGCCTCATTCCAGAAGCAACACAAGGAGGTACTTACAATTTTGACCCAACAGCCAACC TTCAAACAAAAGAATTTAATTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008466 |
| Insert Size | 1566 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008466.5, NP_032492.1 |
| RefSeq Size | 4167 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 16648 |
| UniProt ID | O35344 |
| Gene Summary | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208345 | Kpna3 (tGFP-tagged) - Mouse karyopherin (importin) alpha 3 (Kpna3) |
CNY 5,040.00 |
|
| MR208345 | Kpna3 (Myc-DDK-tagged) - Mouse karyopherin (importin) alpha 3 (Kpna3) |
CNY 3,880.00 |
|
| MR208345L3 | Lenti ORF clone of Kpna3 (Myc-DDK-tagged) - Mouse karyopherin (importin) alpha 3 (Kpna3) |
CNY 6,460.00 |
|
| MR208345L4 | Lenti ORF clone of Kpna3 (mGFP-tagged) - Mouse karyopherin (importin) alpha 3 (Kpna3) |
CNY 6,460.00 |
