Cyp1a1 (NM_001136059) Mouse Untagged Clone

CAT#: MC217414

Cyp1a1 (untagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), transcript variant 2, (10ug)



  "NM_001136059" in other vectors (4)

CNY 6,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Polyclonal Antibody against cytochrome P450 1A1 (mouse)
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AHH; AHRR; CP11; Cyp1a2; CYPIA1; P450-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217414 representing NM_001136059
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTTCCATGTATGGACTTCCAGCCTTCGTGTCAGCCACAGAGCTGCTCCTGGCTGTCACCGTATTCT
GCCTTGGATTCTGGGTGGTCAGAGCCACAAGAACCTGGGTTCCCAAAGGCCTGAAGACTCCACCAGGGCC
CTGGGGCTTGCCCTTCATTGGTCACATGCTGACTGTGGGGAAGAACCCACATCTGTCACTGACACGGCTG
AGTCAGCAGTATGGGGACGTGCTGCAGATCCGCATCGGCTCCACTCCTGTGGTGGTGCTGAGCGGCCTGA
ACACCATCAAGCAGGCCCTGGTGAGGCAGGGAGATGACTTCAAGGGCCGGCCAGACCTCTACAGCTTCAC
ACTTATCACTAATGGCAAGAGCATGACTTTTAACCCAGACTCTGGACCCGTGTGGGCTGCCCGCCGGCGC
CTGGCCCAGAATGCCCTGAAGAGCTTCTCCATAGCCTCGGACCCGACGTCAGCATCCTCTTGCTACTTGG
AGGAGCACGTGAGCAAGGAGGCTAACTATCTCGTCAGCAAACTTCAGAAGGTGATGGCAGAGGTTGGCCA
CTTTGACCCTTACAAGTATTTGGTCGTGTCAGTAGCCAATGTCATCTGTGCCATATGCTTTGGCCAACGT
TATGACCATGATGACCAAGAGCTGCTCAGCATAGTCAATCTGAGCAATGAGTTTGGGGAGGTTACTGGCT
CTGGATACCCAGCTGACTTCATTCCTGTCCTCCGTTACCTGCCTAACTCTTCCCTGGATGCCTTCAAGGA
CTTGAATGATAAGTTCTACAGCTTCATGAAGAAGTTAATCAAAGAGCACTACAGGACATTTGAGAAGGGC
CACATCCGGGACATCACAGACAGCCTCATTGAGCATTGTCAGGACAGGAAGCTGGACGAGAATGCCAATG
TCCAGCTGTCAGATGATAAGGTCATCACGATTGTTTTGGACCTCTTTGGAGCTGGGTTTGACACAGTCAC
AACTGCTATCTCGTGGAGCCTCATGTACCTGGTAACCAACCCTAGGGTACAGAGAAAGATCCAGGAGGAA
CTAGACACAGTGATTGGCAGAGATCGGCAGCCCCGGCTTTCTGACAGACCTCAGCTGCCCTATCTGGAGG
CCTTCATTCTGGAGACCTTCCGGCATTCATCCTTCGTCCCCTTCACCATCCCCCACAGCACCACAAGAGA
TACAAGTCTGAATGGCTTCTATATCCCCAAGGGGTGCTGTGTCTTTGTGAACCAGTGGCAGGTTAACCAT
GACCGGGAACTGTGGGGTGACCCAAACGAGTTCCGGCCTGAAAGGTTTCTCACCCCCAGCGGCACTCTGG
ACAAGCGCTTGAGTGAGAAGGTCACTCTCTTTGGTTTGGGCAAGCGAAAGTGCATCGGAGAGACCATTGG
CCGATCGGAGGTCTTTCTCTTCCTGGCCATCTTGCTGCAGCAAATAGAATTTAAGGTGTCTCCAGGGGAG
AAGGTGGATATGACTCCTACCTATGGGCTGACTTTAAAACACGCCCGCTGTGAACACTTCCAAGTGCAGA
TGCGGTCTTCTGGTCCTCAGCATCTTCAGGCTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001136059
Insert Size 1575 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001136059.2, NP_001129531.1
RefSeq Size 2637 bp
RefSeq ORF 1575 bp
Locus ID 13076
UniProt ID P00184
Gene Summary A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) contains an alternate 5' non-coding exon compared to variant 1. Variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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