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Sgo1 (NM_028232) Mouse Untagged Clone
CAT＃: MC217322
Sgol1 (untagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1), (10ug)
CNY 6,650.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3300001M08Rik; C81037; Sgo1; Sgol1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217322 representing NM_028232
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAGGAAAGGTGTCAGAAAAGGTCCTTTCAAGATACCCTTGAAGACATTAAGAATCGAATGAAAG AAAAAAGGAATAAAAATTTGGCGGGGATTGGGAAACGCAAGTCCTTTATTGTTGCACCGGGCCAAGTACC CACTAACACTGCTACACTACTGAGATATTACCAAGATAACAACAGGTTGTTAGTCTTGGCTTTGGAAAAT GAGAAATCCAAAGTGAGAGAAGCACAGGATGTCATCCTGCAACTGAGAAAAGAATGCTACTACCTTACTT GTCAGCTGTATGCATTGAAAGAGAAGCTAACTTCCCGACAAAGTGAAGAAACTACTCAGAACTGGAAAGG ACGTCCCTCAGACGTGGTCTCCAGCATTGACAATACGACCAGGGACTTGTCAGGGAAGTCCTTACAGCAA ATTGCTGTTGAAGAAACTGATTGTCCTTACCAAACCACAGAACCAAGTCCTGCTGTTACTCCAGAGACAC AGGGTTGCGATTTTGATTCAGGTAAAGTTGAGTCTACTGATGAAGTCTTACCCAGAACTATATCTATCCG TCGCCATTTAAGGAAAGATTTTAGTAATATAAGCCACTCCACGACTTTGGAGGATTGTAAAGCCAGTCCA AGAGTGGCACAGTCTCTGGAAGTTAAAGGAAGTAGATGTAGAGAAGTAACCGTAACCCTGCACAGACTTG AAAATGTTTGTCTGTGGAACAAAGACCAAATTAGCTTATGTTCTAGACTGATTAACCCAGCAAAGATTAC TGAAACAGAAGTCATTTTATCATCTAAACCTGAACAAATAGAAAGCAAGCATAAACGTGCACGAAAAAGA AGAGCAGAGCAAAGAAGAACCAAGCAGAGATGCAAATCAAAATCCTCATTGAGGAGTAAGGGGAACAAAA ACAAAGATAAGCAGGGTTTACCCCCTACTACACTGGATGGAGGTATTGGTTCCTGTGATGCTTACGATTT TAATCTAAAAGGGACGGTCCACCCCACCCCTTTCCGACAAAAAATGAACAATGGCTGCAACAAAGAAACG GATAGCAGCAACTCAGAAGTGAGTGACCTCGAATGCAGTACCTCTGAGGATGAGTCTGATGACCTCTACC TGCCTCCCTCCAAGCGCTTGCGAGACTACAGAGAGTCAGAGAGAGCAGTTACCAGGCCTCGGTCTAAAAG AGGACTTCAGTACCCAGATGGGAAAGAGAGGAAGGAGGTGCTGCCATCTACAGCTCCTACTGGTATCCCA CCTGAGACTCAAGAGTCACCTCGTTGTAGCCTAAAGGATGTCACCAATATCCTGCAGTGTCCTAGAGTGA AGATCAGGAAGCCTTCTCTGCCTCCAAAGCGGCGTGAAGACAGCCCAGCAGTGGCTCTGACTAAACGCAG GTGTAGCACCATCAAAAGCTATAAAGAGCCAACACTCGCTTCAAAGCTAAGAAGAGGGGACCCTTTCACG GACTTGTGTTTCTTGAATTCTCCTATTTTCAAGCAGAAAAGGGGTATGAGATGTCCTAAAAGAAGAACCA AGCAAACACAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028232 |
| Insert Size | 1554 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028232.2, NP_082508.1 |
| RefSeq Size | 3609 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 72415 |
| UniProt ID | Q9CXH7 |
| Gene Summary | Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Involved in centromeric enrichment of AUKRB in prometaphase.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208287 | Sgol1 (tGFP-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) |
CNY 4,940.00 |
|
| MR208287 | Sgol1 (Myc-DDK-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) |
CNY 3,848.00 |
|
| MR208287L3 | Lenti ORF clone of Sgol1 (Myc-DDK-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) |
CNY 6,370.00 |
|
| MR208287L4 | Lenti ORF clone of Sgol1 (mGFP-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) |
CNY 6,370.00 |
