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Usp44 (NM_183199) Mouse Untagged Clone
CAT＃: MC217222
Usp44 (untagged) - Mouse ubiquitin specific peptidase 44 (Usp44), (10ug)
CNY 6,560.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | E430004F17Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217222 representing NM_183199
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGGTGCAAGCACGTTGAGCAGTTGCAGCTTGCTCAAGGCCATTCCATCTTGGACCCTCAGAAAT GGTACTGTATGGTCTGCAACACAACCGAGTCCATTTGGGCTTGCCTCAGCTGTTCCCACGTCGCCTGTGG GAAGTACATCCAAGAGCACGCACTGAAGCACTTTCAAGAAAGCAGCCATCCCGTTGCCTTCGAGGTGAAC GACATGTACGCTTTTTGTTATCTTTGTAACGATTATGTTCTAAACGATAACGCAGCCGGAGACCTGAAGT CACTACGGAGTACACTAAGTACAATCAAAAGTAAAAAGTACCCCTGTGTGGTTCCGAGTGACTCGGTTTT ACATCCTGTGGATGCACAGGACCGTGTTTACTCCTTACTCGACGGCACCCAATCTCTGCCCGGAAACGAG GATCCAACGTGCGCCGCCCTCTGGCACAGGAGACGGGTGCTCATGGGGAAAGCCTTTCGAACTTGGTTCG AACAGTCAGCAATTGGAAGGAAAGGGCAAGAGCCAACTCAGGAGAGAATGGTAGCAAAACGAGAGGCGAA GAGAAGGCAGCAGCAGGAGTTGGAGCAGCAGATGAAAGCCGAGCTGGAAAGCACACCTCCGCGGAAGAGT TTACGCTTACAAGGCTCCTCGGAGGAGGCGGCCACAATCGAAATCGTGCCAGTGCGGGCACCGCCCCCAC CTCCCGCATCCCCAGCAAAGGACAAAGCCGCGCTACCTACTTCGGAAGACAGGACTTTTAAAAAACTTGA CCTGAACCAGTGGCTGGCTGTGGCTGCCAGCGATAAGGCCCGATCCTATAAGCACTCAGCCGTCACGGAG GCGGCGGCACAGCAGATGAACGAAGGGCAAGAGAAAGAGAAAGGCTTCGTATGCTCCAGACATTCGGGTT TATCCTCAGGCCTGAGCGGAGGGGCCTCAAAAGGTCGGAACATGGAGCTTATTCAGCCAAGGGAGCCCAG TTCCCCGTACAGTTCTCTCTGCCATGAATTGCATATCCTGTTCCAAGTCATGTGGTCTGGAGAGTGGGCC TTGGTCTCACCGTTTGCCATGCTTCACTCCGTGTGGAGGCTGATCCCTGCTTTCCGCGGTTACGCCCAGC AGGATGCTCAGGAATTTCTTTGTGAACTTCTGGATAAGATACAACGTGAACTAGAGACAACCGGAACCAA GTTACCAGCCCTCATCCCCACTTCCCAAAGGAGACTTATTGAGCAAGTTCTGAATGTGGTGAATAACATT TTTCACGGACAATTTCTTAGTCAGGTATGGATGTCTTGCCACATTTTTATCATCTTCGGCAATTCCTACA GTGCGGCCTACAACTGGGTATTCTGTATGGATCGAGCGTGGGAAAGTTGGTGGCATTTCTCGGTCAGAAT AGATTTCTTTCACGAGTGTGTGAAGAAGTCACGGATCTCTGAATCATTGGAAATAGTTTTGTATTTGTGG AAGCAGTGGCTTTATGGGGGAGAGAGTAAGGAGAACTGCATCTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_183199 |
| Insert Size | 1518 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_183199.3, NP_899022.2 |
| RefSeq Size | 2946 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 327799 |
| Gene Summary | Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222450 | Usp44 (tGFP-tagged) - Mouse ubiquitin specific peptidase 44 (Usp44), (10ug) |
CNY 5,040.00 |
|
| MR222450 | Usp44 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 44 (Usp44) |
CNY 3,760.00 |
|
| MR222450L3 | Lenti ORF clone of Usp44 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 44 (Usp44) |
CNY 6,460.00 |
|
| MR222450L4 | Lenti ORF clone of Usp44 (mGFP-tagged) - Mouse ubiquitin specific peptidase 44 (Usp44) |
CNY 6,460.00 |
