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Mitf (NM_001178049) Mouse Untagged Clone
CAT＃: MC217141
Mitf (untagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 3, (10ug)
CNY 3,808.00
CNY 6,560.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCC2; Bhlhe32; bw; Gsfbcc2; mi; vit; Vitiligo; Wh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_001178049.1
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCTTAGATTTGAGATGCTCATCCCCTGCTCCTTTGAAAGCTTGTGTCTCAGTTCTGCAGAGC ATTCTGGGGCCTCCAAGCCTCCGTTAAGCTCCTCCACTATGACATCACGCATCTTGCTACGCCAGCAACT CATGCGTGAGCAGATGCAGGAGCAGGAGCGCAGGGAGCAGCAGCAGAAGCTGCAGGCAGCCCAGTTCATG CAACAGAGAGTGGCCGTGAGTCAGACACCAGCCATAAACGTCAGCGTGCCCACCACCCTTCCCTCTGCCA CCCAGGTGCCGATGGAAGTCCTTAAGGTGCAGACCCACCTGGAAAACCCCACCAAGTACCACATACAGCA AGCTCAGAGGCACCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAGCCAAGTCCTG AGCTCACCATGTCCAAACCAGCCTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGCACCCAACA GCCCTATGGCTATGCTCACTCTTAACTCCAACTGTGAAAAAGAGGCATTTTATAAGTTTGAGGAGCAGAG CAGGGCAGAGAGTGAGTGCCCAGGTATGAACACGCACTCTCGAGCGTCGTGCATGCAGATGGATGATGTA ATTGATGACATCATCAGCCTGGAATCAAGTTATAATGAAGAAATTTTGGGCTTGATGGATCCGGCCTTGC AAATGGCAAATACGTTACCCGTCTCTGGAAACTTGATCGACCTCTACAGCAACCAGGGCCTGCCACCGCC AGGCCTTACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAACATAAAAAGGGAGCTCACAGCGTGTATT TTCCCCACAGAGTCTGAAGCAAGAGCATTGGCTAAAGAGAGGCAGAAAAAGGACAATCACAACTTGATTG AACGAAGAAGAAGATTTAACATAAACGACCGCATTAAGGAGCTAGGTACTCTGATCCCCAAGTCAAATGA TCCAGACATGCGGTGGAACAAGGGAACCATTCTCAAGGCCTCTGTGGACTACATCCGGAAGTTGCAACGG GAACAGCAACGAGCTAAGGACCTTGAAAACCGACAGAAGAAGCTGGAGCATGCGAACCGGCACCTGCTGC TCAGAGTACAGGAGCTGGAGATGCAGGCTAGAGCGCATGGACTTTCCCTTATCCCATCCACCGGTCTCTG CTCGCCTGATCTGGTGAATCGGATCATCAAGCAAGAACCAGTTCTTGAGAACTGCAGCCAGGAACTTGTA CAGCACCAGGCAGACCTGACATGTACGACAACTCTGGATCTCACGGACGGTACCATCACCTTTACCAACA ACCTCGGCACCATGCCGGAGAGCAGCCCGGCCTACAGCATCCCCAGGAAGATGGGCTCCAACTTGGAAGA CATCCTGATGGACGATGCCCTCTCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTCGCCAGGA GCTTCAAAAACAAGCAGCCGGAGGAGCAGTATGAGCGCAGAAGAAACGGAGCATGCGTGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001178049 |
| Insert Size | 1533 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001178049.1, NP_001171520.1 |
| RefSeq Size | 4777 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 17342 |
| Gene Summary | This transcription factor serves at a critical point between extracellular signaling and downstream targets in cell specification in early eye and neural crest development. Mutant alleles have been identified that generate distinct phenotypes. Some of these alleles are being used to model the human diseases Waardenburg syndrome IIa and Tietz syndrome. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3, also known as Mitf-H) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226997 | Mitf (tGFP-tagged) - Mouse microphthalmia-associated transcription factor (Mitf) transcript variant 3, (10ug) |
CNY 5,610.00 |
|
| MR226997 | Mitf (Myc-DDK-tagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 3 |
CNY 5,130.00 |
|
| MR226997L3 | Lenti ORF clone of Mitf (Myc-DDK-tagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 3 |
CNY 7,030.00 |
|
| MR226997L4 | Lenti ORF clone of Mitf (mGFP-tagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 3 |
CNY 7,030.00 |
