Srf (NM_020493) Mouse Untagged Clone
CAT#: MC217127
Srf (untagged) - Mouse serum response factor (Srf), (10ug)
CNY 3,760.00
CNY 6,560.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AW049942; AW240594 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC217127 representing NM_020493
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTACCGAGCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAACCTGAACC GGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACTCGCGGGGCGAACGGGGGCCGGGTCCCCGGGAA CGGCGCGGGGCTCGGCCAGAGTCGTCTGGAGCGGGAGGCTGCAGCGGCAGCGGCGCCCACCGCCGGGGCC CTCTACAGCGGCAGCGAGGGCGACTCCGAGTCCGGCGAGGAGGAGGAGCTGGGCGCCGAGCGGCGCGGCC TCAAGCGGAGCCTGAGCGAGATGGAGCTCGGCGTGGTGGTCGGTGGGCCTGAGGCGGCGGCGGCGGCCGC CGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGGGCCAAGCCGGGGAAGAAGACCCGGGGCCGCGTG AAGATCAAGATGGAGTTCATCGACAACAAGCTGCGGCGCTACACGACCTTCAGCAAGAGGAAGACGGGCA TCATGAAGAAGGCCTATGAGCTGTCCACGCTGACAGGGACACAGGTGCTGTTGCTGGTGGCCAGTGAGAC AGGCCATGTGTATACCTTTGCCACCCGCAAACTGCAGCCCATGATCACCAGTGAGACCGGCAAGGCGCTG ATTCAGACCTGCCTCAACTCGCCAGACTCTCCGCCCCGCTCAGACCCCACCACAGACCAGAGAATGAGTG CCACTGGCTTTGAAGAGCCAGATCTCACCTACCAGGTGTCGGAATCTGACAGCAGTGGGGAAACCAAGGA CACACTGAAGCCAGCATTCACAGTCACCAACCTGCCGGGTACCACCTCCACAATCCAGACAGCACCCAGC ACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCCTTCCCCATCACCAACTACCTGGCACCAGTGTCTG CTAGTGTCAGCCCCAGCGCTGTCAGCAGTGCCAACGGGACTGTGCTCAAGAGTACAGGCAGCGGCCCTGT CTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGCTTCACCCTCATGCCTGGTGGGGCAGTGGCCCAGCAG GTCCCTGTGCAGGCCATTCATGTGCACCAGGCCCCACAGCAAGCGTCTCCCTCTCGTGACAGCAGCACAG ACCTCACGCAGACCTCCTCCAGCGGGACAGTGACGTTGCCCGCCACCATCATGACGTCGTCTGTACCCAC AACTGTGGGTGGCCACATGATGTACCCTAGTCCCCATGCAGTGATGTATGCCCCCACCTCAGGCCTGGCT GATGGCAGCCTCACCGTGCTCAATGCCTTCTCTCAGGCACCATCCACCATGCAGGTGTCCCACAGCCAGG TCCAGGAGCCAGGTGGTGTCCCTCAGGTGTTCCTGACAGCACCGTCTGGGACCGTGCAGATCCCTGTCTC TGCAGTTCAGCTTCACCAGATGGCTGTGATAGGGCAGCAAGCTGGGAGCAGCAGCAACCTCACCGAGCTA CAGGTGGTGAACCTGGATGCCACCCACAGCACCAAGAGTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_020493 |
Insert Size | 1515 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC051950, AAH51950 |
RefSeq Size | 2634 bp |
RefSeq ORF | 1515 bp |
Locus ID | 20807 |
UniProt ID | Q9JM73 |
Gene Summary | SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). Required for cardiac differentiation and maturation (PubMed:15169892).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A mechanism of cooling hot tumors: Lactate attenuates inflammation in dendritic cells
,null,
iScience
,PubMed ID 34541473
[Srf]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG208120 | Srf (tGFP-tagged) - Mouse serum response factor (Srf) |
CNY 7,232.00 |
|
MR208120 | Srf (Myc-DDK-tagged) - Mouse serum response factor (Srf) |
CNY 5,632.00 |
|
MR208120L1 | Lenti ORF clone of Srf (Myc-DDK-tagged) - Mouse serum response factor (Srf) |
CNY 6,940.00 |
|
MR208120L2 | Lenti ORF clone of Srf (mGFP-tagged) - Mouse serum response factor (Srf) |
CNY 8,032.00 |
|
MR208120L3 | Lenti ORF clone of Srf (Myc-DDK-tagged) - Mouse serum response factor (Srf) |
CNY 6,940.00 |
|
MR208120L4 | Lenti ORF clone of Srf (mGFP-tagged) - Mouse serum response factor (Srf) |
CNY 8,032.00 |