Srf (NM_020493) Mouse Untagged Clone

CAT#: MC217127

Srf (untagged) - Mouse serum response factor (Srf), (10ug)



  "NM_020493" in other vectors (6)

CNY 3,760.00

CNY 6,560.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW049942; AW240594
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217127 representing NM_020493
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTACCGAGCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAACCTGAACC
GGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACTCGCGGGGCGAACGGGGGCCGGGTCCCCGGGAA
CGGCGCGGGGCTCGGCCAGAGTCGTCTGGAGCGGGAGGCTGCAGCGGCAGCGGCGCCCACCGCCGGGGCC
CTCTACAGCGGCAGCGAGGGCGACTCCGAGTCCGGCGAGGAGGAGGAGCTGGGCGCCGAGCGGCGCGGCC
TCAAGCGGAGCCTGAGCGAGATGGAGCTCGGCGTGGTGGTCGGTGGGCCTGAGGCGGCGGCGGCGGCCGC
CGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGGGCCAAGCCGGGGAAGAAGACCCGGGGCCGCGTG
AAGATCAAGATGGAGTTCATCGACAACAAGCTGCGGCGCTACACGACCTTCAGCAAGAGGAAGACGGGCA
TCATGAAGAAGGCCTATGAGCTGTCCACGCTGACAGGGACACAGGTGCTGTTGCTGGTGGCCAGTGAGAC
AGGCCATGTGTATACCTTTGCCACCCGCAAACTGCAGCCCATGATCACCAGTGAGACCGGCAAGGCGCTG
ATTCAGACCTGCCTCAACTCGCCAGACTCTCCGCCCCGCTCAGACCCCACCACAGACCAGAGAATGAGTG
CCACTGGCTTTGAAGAGCCAGATCTCACCTACCAGGTGTCGGAATCTGACAGCAGTGGGGAAACCAAGGA
CACACTGAAGCCAGCATTCACAGTCACCAACCTGCCGGGTACCACCTCCACAATCCAGACAGCACCCAGC
ACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCCTTCCCCATCACCAACTACCTGGCACCAGTGTCTG
CTAGTGTCAGCCCCAGCGCTGTCAGCAGTGCCAACGGGACTGTGCTCAAGAGTACAGGCAGCGGCCCTGT
CTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGCTTCACCCTCATGCCTGGTGGGGCAGTGGCCCAGCAG
GTCCCTGTGCAGGCCATTCATGTGCACCAGGCCCCACAGCAAGCGTCTCCCTCTCGTGACAGCAGCACAG
ACCTCACGCAGACCTCCTCCAGCGGGACAGTGACGTTGCCCGCCACCATCATGACGTCGTCTGTACCCAC
AACTGTGGGTGGCCACATGATGTACCCTAGTCCCCATGCAGTGATGTATGCCCCCACCTCAGGCCTGGCT
GATGGCAGCCTCACCGTGCTCAATGCCTTCTCTCAGGCACCATCCACCATGCAGGTGTCCCACAGCCAGG
TCCAGGAGCCAGGTGGTGTCCCTCAGGTGTTCCTGACAGCACCGTCTGGGACCGTGCAGATCCCTGTCTC
TGCAGTTCAGCTTCACCAGATGGCTGTGATAGGGCAGCAAGCTGGGAGCAGCAGCAACCTCACCGAGCTA
CAGGTGGTGAACCTGGATGCCACCCACAGCACCAAGAGTGAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_020493
Insert Size 1515 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC051950, AAH51950
RefSeq Size 2634 bp
RefSeq ORF 1515 bp
Locus ID 20807
UniProt ID Q9JM73
Gene Summary SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). Required for cardiac differentiation and maturation (PubMed:15169892).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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