Dars (NM_177445) Mouse Untagged Clone

Name: Dars (NM_177445) Mouse Untagged Clone
SKU: MC217054
Price: 6460.00

CAT＃: MC217054

Dars (untagged) - Mouse aspartyl-tRNA synthetase (Dars), transcript variant 1, (10ug)

Datasheet

SDS

"NM_177445" in other vectors (3)

CNY 6,460.00

货期*

5周

规格

Product images

经常一起买 (3)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730439G15Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC217054 representing NM_177445 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGCACCAACGCCAGCCGTAAGGGTCAGGAGAAGCCGCGGGAGATCGTGGACGCGGCGGAAGATT ATGCTAAAGAGAGATATGGGATATCTTCTATGATACAATCACAAGAAAAGCCAGATAGAGTTTTGGTTCG AGTTAAGGACCTGACAGTTCAAAAAGCTGATGATGTTGTTTGGGTCCGTGCAAGAGTTCATACAAGCAGA GCAAAAGGGAAGCAGTGCTTCTTAGTCCTGAGGCAGCAGCAGTTTAATGTCCAGGCTCTTGTGGCGGTGG GAGACCATGCAAGCAAGCAGATGGTTAAATTTGCTGCCAACATCAACAAAGAAAGTATTATTGACGTAGA AGGTGTTGTGAGGAAAGTCAATCAGAAAATTGGAAGCTGTACCCAGCAGGATGTTGAGTTGCATGTCCAG AAGATTTATGTGATCAGCTTGGCTGAACCTCGCCTGCCCTTGCAGCTGGATGATGCCATTCGGCCTGAAG TGGAAGGAGAAGAGGACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCT TAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCATCTGCCATCTCTTCCGAGAAACTTTA ATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATAATTTCAGCTGCCAGTGAAGGAGGAGCCAATG TTTTTACTGTGTCCTATTTTAAAAATAATGCCTACCTTGCTCAGTCTCCACAATTGTATAAGCAGATGTG CATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCAT AGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAG AAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAAC TGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAG GCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAGTACACCAAATGAAA AACTGTTGGGTCGTTTGGTGAAGGAAAAGTATGATACAGACTTTTATGTTCTTGATAAATATCCATTGGC TGTAAGACCTTTCTATACCATGCCTGACCCAAGAAATCCGAAACAGTCCAACTCTTACGATATGTTCATG AGAGGAGAAGAAATATTGTCTGGAGCACAAAGAATCCATGATCCTCAGTTGCTTACAGAGAGAGCTTTAC ACCATGGAATTGATTTGGAGAAAATCAAAGCTTACATTGATTCCTTCCGCTTTGGAGCTCCTCCTCATGC TGGTGGTGGCATTGGGCTGGAAAGGGTGACGATGCTGTTTTTAGGATTGCATAACGTACGCCAGACCTCA ATGTTCCCTCGTGACCCCAAACGACTCACGCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177445
Insert Size	1506 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_177445.5, NP_803228.2
RefSeq Size	2249 bp
RefSeq ORF	1506 bp
Locus ID	226414
UniProt ID	Q922B2
Gene Summary	Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MR208055	Dars (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase (Dars), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,040.00
MR208055L3	Lenti ORF clone of Dars (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase (Dars), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,940.00
MR208055L4	Lenti ORF clone of Dars (mGFP-tagged) - Mouse aspartyl-tRNA synthetase (Dars), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,940.00

其它Dars产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies