Celf4 (NM_001146294) Mouse Untagged Clone

CAT#: MC217037

Celf4 (untagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 3, (10ug)



  "NM_001146294" in other vectors (4)

CNY 6,460.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A230070D14Rik; Brul4; BRUNOL-4; Brunol4; C130060B05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217037 representing NM_001146294
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATATAAAGATGGCCACGTTAGCAAACGGACAGGCTGACAACGCGAGCCTCAGTACCAACGGGCTAG
GCAGCAGCCCGGGCAGCGCCGGGCATATGAACGGATTAAGCCACAGCCCGGGGAACCCGTCGACCATTCC
CATGAAGGACCACGATGCCATCAAGCTGTTCATTGGGCAGATCCCCCGAAACCTGGATGAGAAGGACCTC
AAGCCCCTCTTCGAGGAGTTCGGCAAGATCTACGAGCTTACGGTTCTGAAGGACAGGTTCACAGGCATGC
ACAAAGGCTGCGCTTTCCTCACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCCCAGAGCGCGCTGCACGA
GCAGAAGACCCTGCCCGGGATGAACCGGCCGATCCAGGTGAAGCCTGCGGACAGCGAGAGCCGAGGAGAT
AGAAAACTCTTCGTGGGTATGCTCAACAAGCAACAATCTGAGGACGACGTGCGCCGCCTCTTCGAGGCCT
TCGGGAACATCGAGGAGTGCACTATCCTGCGCGGGCCGGACGGCAACAGCAAGGGGTGCGCCTTTGTGAA
GTACTCCTCCCATGCCGAGGCACAAGCCGCCATCAACGCTCTACATGGCAGCCAGACCATGCCTGGAGCC
TCCTCCAGCCTGGTGGTCAAGTTTGCAGACACTGACAAGGAGCGCACAATGCGACGGATGCAGCAGATGG
CTGGCCAGATGGGCATGTTCAACCCCATGGCCATCCCCTTCGGAGCCTATGGCGCCTATGCTCAGGCACT
GATGCAGCAGCAAGCAGCCCTCATGGCATCGGTCGCGCAAGGAGGCTACCTGAATCCCATGGCTGCCTTC
GCTGCCGCCCAAATGCAGCAGATGGCGGCCCTCAACATGAATGGCCTGGCAGCCGCACCTATGACCCCAA
CCTCAGGTGGCAGCACCCCTCCAGGCATCACTGCACCAGCTGTGCCTAGCATCCCATCCCCCATTGGGGT
GAACGGCTTCACGGGCCTCCCCCCTCAGGCCAATGGGCAGCCTGCTGCGGAAGCTGTGTTTGCCAATGGC
ATTCACCCCTACCCAGCACAGAGCCCCACCGCAGCCGACCCCCTGCAGCAGGCCTACGCTGGAGTGCAGC
AGTATGCAGGACCAGCTGCCTACCCTGCTGCCTATGGTCAGATTAGCCAGGCCTTTCCTCAGCCACCGCC
AATGATTCCCCAGCAACAGAGAGAAGGGCCCGAGGGCTGTAACCTGCTCATCTACCATCTGCCCCAGGAG
TTTGGGGACGCTGAGCTGATGCAGATGTTCCTCCCTTTCGGTAATGTCATCTCCTCGAAAGTGTTTGTGG
ATCGGGCGACTAACCAAAGTAAATGCTTTGGCTTCGTGAGCTTCGACAACCCGGCCAGCGCACAGACCGC
CATCCAGGCCATGAACGGCTTCCAGATAGGCATGAAGAGGCTCAAGGTGCAGCTGAAGCGGCCCAAAGAC
GCCAATCGCCCGTACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001146294
Insert Size 1488 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001146294.1, NP_001139766.1
RefSeq Size 3980 bp
RefSeq ORF 1488 bp
Locus ID 108013
UniProt ID Q7TSY6
Gene Summary RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) encodes isoform (29, also known as C).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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