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Hrh1 (NM_008285) Mouse Untagged Clone
CAT＃: MC216800
Hrh1 (untagged) - Mouse histamine receptor H1 (Hrh1), (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Bphs; H1R; HH1R; Hir |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216800 representing NM_008285
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTTCCCAACACCTCTTCTGCCTCCGAAGACAAGATGTGTGAGGGGAACAGGACAGCCATGGCCA GCCCTCAGCTGCTGCCCCTGGTGGTGGTTCTAAGTAGTATCTCCCTGGTCACAGTGGGCCTCAACCTGCT GGTGCTGTATGCAGTGCGCAGTGAGCGCAAGCTACACACCGTGGGCAACCTGTACATTGTCAGCCTGTCG GTAGCAGACCTGATTGTAGGGGCAGTCGTCATGCCCATGAACATCCTCTATCTTATCATGACCAAGTGGT CCCTGGGCCGCCCCCTCTGCCTCTTTTGGCTCTCTATGGATTATGTGGCCAGCACGGCATCCATCTTTAG TGTCTTCATCCTGTGTATTGATCGCTACCGCTCTGTCCAGCAACCCCTCCGGTACCTGAGGTATCGAACC AAGACCCGTGCTTCAGCTACCATCCTGGGGGCCTGGTTTCTCTCCTTCCTGTGGGTTATACCTATACTTG GCTGGCATCACTTCACGCCCCTGGCCCCAGAGCTTCGGGAAGATAAGTGTGAGACAGATTTCTACAATGT CACTTGGTTCAAGATCATGACCGCCATCATCAACTTCTACCTCCCCACTTTGCTCATGCTGTGGTTCTAT GTGAAGATCTACAAGGCTGTGCGGCGACACTGTCAGCACCGCCAGCTCACCAACGGGTCCCTCCCTACCT TTTTAGAAATCAAGCTGAGGTCGGAGGATGCCAAAGAGGGTGCCAAGAAACCTGGGAAAGAGTCTCCCTG GGGGGTCCAGAAGAGGCCGTCAAGAGACCCTACTGGAGGTCTGGATCAGAAGTCAACATCTGAAGACCCC AAGGTGACCTCTCCGACTGTCTTCAGCCAAGAGGGGGAAAGGGAAACAGTCACACGCCCCTGTTTCCGTC TTGACGTCATGCAGACACAGCCTGTGCCTGAGGGAGATGCCAGGGGCTCAAAGGCCAATGACCAGACCTT GAGCCAGCCCAAAATGGATGAGCAGAGCCTGAGTACTTGCCGGCGGATCAGTGAGACATCAGAGGACCAG ACCTTGGTGGATCGACAGTCCTTCTCCCGGACCACAGACTCAGACACCAGCATAGAGCCAGGGCTGGGCA AAGTCAAAGCGAGAAGCAGGTCTAACAGTGGCCTGGACTACATCAAAGTCACCTGGAAGAGGCTTCGCTC ACATTCCAGACAGTATGTGTCCGGGTTGCACTTGAACCGAGAGCGGAAGGCAGCCAAGCAGTTGGGTTGT ATCATGGCAGCATTCATTCTCTGCTGGATTCCCTATTTCATCTTCTTCATGGTCATTGCCTTCTGCAACA GCTGCTGCAGCGAACCTGTGCACATGTTCACCATTTGGCTGGGCTACATCAACTCCACGCTGAACCCCCT CATCTACCCGCTGTGCAACGAGAACTTCAAGAAGACATTCAAAAAAATTCTGCACATTCGTTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008285 |
| Insert Size | 1467 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008285.3, NP_032311.2 |
| RefSeq Size | 3782 bp |
| RefSeq ORF | 1467 bp |
| Locus ID | 15465 |
| UniProt ID | P70174 |
| Gene Summary | In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Involved in circadian rhythm of locomotor activity and exploratory behavior. Also involved in responsiveness to pertussis toxin through its control of susceptibility to histamine hypersensitivity and enhancement of antigen-specific delayed-type hypersensitivity responses.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 6. Variants 1 through 6 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226450 | Hrh1 (tGFP-tagged) - Mouse histamine receptor H1 (Hrh1), (10ug) |
CNY 5,320.00 |
|
| MR226450 | Hrh1 (Myc-DDK-tagged) - Mouse histamine receptor H1 (Hrh1) |
CNY 5,488.00 |
|
| MR226450L3 | Lenti ORF clone of Hrh1 (Myc-DDK-tagged) - Mouse histamine receptor H1 (Hrh1) |
CNY 6,750.00 |
|
| MR226450L4 | Lenti ORF clone of Hrh1 (mGFP-tagged) - Mouse histamine receptor H1 (Hrh1) |
CNY 6,750.00 |
