Celf4 (NM_133195) Mouse Untagged Clone

CAT#: MC216700

Celf4 (untagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 4, (10ug)



  "NM_133195" in other vectors (5)

CNY 6,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A230070D14Rik; Brul4; BRUNOL-4; Brunol4; C130060B05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216700 representing NM_133195
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATATAAAGATGGCCACGTTAGCAAACGGACAGGCTGACAACGCGAGCCTCAGTACCAACGGGCTAG
GCAGCAGCCCGGGCAGCGCCGGGCATATGAACGGATTAAGCCACAGCCCGGGGAACCCGTCGACCATTCC
CATGAAGGACCACGATGCCATCAAGCTGTTCATTGGGCAGATCCCCCGAAACCTGGATGAGAAGGACCTC
AAGCCCCTCTTCGAGGAGTTCGGCAAGATCTACGAGCTTACGGTTCTGAAGGACAGGTTCACAGGCATGC
ACAAAGGCTGCGCTTTCCTCACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCCCAGAGCGCGCTGCACGA
GCAGAAGACCCTGCCCGGGATGAACCGGCCGATCCAGGTGAAGCCTGCGGACAGCGAGAGCCGAGGAGGT
AGTAGCTGCCTGCGCCAGCCCCCTTCACATAGAAAACTCTTCGTGGGTATGCTCAACAAGCAACAATCTG
AGGACGACGTGCGCCGCCTCTTCGAGGCCTTCGGGAACATCGAGGAGTGCACTATCCTGCGCGGGCCGGA
CGGCAACAGCAAGGGGTGCGCCTTTGTGAAGTACTCCTCCCATGCCGAGGCACAAGCCGCCATCAACGCT
CTACATGGCAGCCAGACCATGCCTGGAGCCTCCTCCAGCCTGGTGGTCAAGTTTGCAGACACTGACAAGG
AGCGCACAATGCGACGGATGCAGCAGATGGCTGGCCAGATGGGCATGTTCAACCCCATGGCCATCCCCTT
CGGAGCCTATGGCGCCTATGCTCAGGCACTGATGCAGCAGCAAGCAGCCCTCATGGCATCGGTCGCGCAA
GGAGGCTACCTGAATCCCATGGCTGCCTTCGCTGCCGCCCAAATGCAGCAGATGGCGGCCCTCAACATGA
ATGGCCTGGCAGCCGCACCTATGACCCCAACCTCAGGTGGCAGCACCCCTCCAGGCATCACTGCACCAGC
TGTGCCTAGCATCCCATCCCCCATTGGGGTGAACGGCTTCACGGGCCTCCCCCCTCAGGCCAATGGGCAG
CCTGCTGCGGAAGCTGTGTTTGCCAATGGCATTCACCCCTACCCAGCACAGAGCCCCACCGCAGCCGACC
CCCTGCAGCAGGCCTACGCTGGAGTGCAGCAGTATGCAGGACCTGCCTACCCTGCTGCCTATGGTCAGAT
TAGCCAGGCCTTTCCTCAGCCACCGCCAATGATTCCCCAGCAACAGAGAGAAGGGCCCGAGGGCTGTAAC
CTGCTCATCTACCATCTGCCCCAGGAGTTTGGGGACGCTGAGCTGATGCAGATGTTCCTCCCTTTCGGCT
TCGTGAGCTTCGACAACCCGGCCAGCGCACAGACCGCCATCCAGGCCATGAACGGCTTCCAGATAGGCAT
GAAGAGGCTCAAGGTGCAGCTGAAGCGGCCCAAAGACGCCAATCGCCCGTACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133195
Insert Size 1455 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133195.3, NP_573458.2
RefSeq Size 3947 bp
RefSeq ORF 1455 bp
Locus ID 108013
UniProt ID Q7TSY6
Gene Summary RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (4) encodes isoform (41, also known as D).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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