Ulk3 (NM_027895) Mouse Untagged Clone

CAT#: MC216515

Ulk3 (untagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3), (10ug)



  "NM_027895" in other vectors (4)

CNY 6,180.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1200015E14Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216515 representing NM_027895
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGGCCCAGCTGGGGTCTCCCTCGGCTGGACGGTTTCATCCTTACCGAGCGCCTGGGCAGTGGCA
CGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGATACTCGGGAAGTGGTAGCCATAAAATGCGTGGC
CAAGAAGAGTCTCAACAAGGCGTCAGTGGAAAACCTCCTGACTGAGATTGAGATCCTCAAGGGCATTCGG
CACCCCCATATCGTGCAGCTGAAAGACTTCCAGTGGGACAATGACAATATCTACCTCATCATGGAGTTCT
GTGCAGGGGGTGACCTGTCTCGCTTCATTCATACCCGCAGGATCCTGCCTGAGAAGGTGGCCCGTGTTTT
CATGCAGCAGTTGGCTAGTGCCCTGCAGTTCCTGCATGAACGAAACATCTCTCACTTGGATCTGAAACCG
CAGAACATCCTGCTGAGCTCTTTGGAGAAGCCCCACCTGAAACTGGCAGACTTTGGCTTTGCCCAGCACA
TGTCCCCGTGGGACGAAAAACACGTGCTCCGTGGCTCCCCGCTCTATATGGCTCCTGAGATGGTGTGTCG
GCGGCAGTATGATGCGCGTGTGGACCTCTGGTCTGTGGGGGTGATCCTGTACGAAGCCCTCTTTGGGCAG
CCCCCCTTTGCCTCCAGATCGTTCTCAGAGCTAGAAGAAAAGATTCGCAGCAATCGGGTGATTGAGCTCC
CTCTTCGGCCCCAACTCTCCCTAGACTGCCGGGACCTGTTGCAGCGACTTCTAGAGCGGGACCCCGCCCG
TCGAATCTCCTTCAAGGACTTCTTTGCCCATCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGC
CTGGCACAGGCAAGGGCCCTTGTGGTGGAGGCTGTGAAGAAGGACCAGGAGGGGGATGCTGCCGCTGCCC
TGTCGCTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCGCTACACTACGAAGTGGATGCCCAGAGGAA
GGAGGCAATTAAGGCGAAGGTGGGACAGTATGTGTCCCGGGCAGAGGAGCTCAAAGCCATTGTCTCCTCC
TCCAATCAGGCCCTGCTAAGACAGGGCACAACTGTCCAAGAGCTGCTTCGAGAGATGGCCCGTGACAAAC
CACGCCTCCTGGCTGCCCTGGAAGTGGCCTCAGCTGCCCTGGCCAAGGAGGAGGAAGCTGGCAAAGAGCA
GGATGCCCTGGACCTGTACCAGCACAGCCTCGGGGAGCTGCTAGTGCTGTTGGCAGCAGAGGCCCCAGGC
CGAAGGCGGGAGCTCCTTCACACCGAGGTTCAGAACCTCATGGCTCGAGCTGAATACCTGAAGGAGCAGA
TCAAGATAAGGGAGTCTCACTGGGAAGCGGAGAGTCTGGACAAAGAGGGGCTGTCGGAGTCTGTTCGTAG
TTCTTGCACACTGCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027895
Insert Size 1419 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027895.1, NP_082171.1
RefSeq Size 2826 bp
RefSeq ORF 1419 bp
Locus ID 71742
UniProt ID Q3U3Q1
Gene Summary Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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