United States
Germany
Japan
United Kingdom
China
Ulk3 (NM_027895) Mouse Untagged Clone
CAT＃: MC216515
Ulk3 (untagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3), (10ug)
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1200015E14Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216515 representing NM_027895
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGCCCAGCTGGGGTCTCCCTCGGCTGGACGGTTTCATCCTTACCGAGCGCCTGGGCAGTGGCA CGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGATACTCGGGAAGTGGTAGCCATAAAATGCGTGGC CAAGAAGAGTCTCAACAAGGCGTCAGTGGAAAACCTCCTGACTGAGATTGAGATCCTCAAGGGCATTCGG CACCCCCATATCGTGCAGCTGAAAGACTTCCAGTGGGACAATGACAATATCTACCTCATCATGGAGTTCT GTGCAGGGGGTGACCTGTCTCGCTTCATTCATACCCGCAGGATCCTGCCTGAGAAGGTGGCCCGTGTTTT CATGCAGCAGTTGGCTAGTGCCCTGCAGTTCCTGCATGAACGAAACATCTCTCACTTGGATCTGAAACCG CAGAACATCCTGCTGAGCTCTTTGGAGAAGCCCCACCTGAAACTGGCAGACTTTGGCTTTGCCCAGCACA TGTCCCCGTGGGACGAAAAACACGTGCTCCGTGGCTCCCCGCTCTATATGGCTCCTGAGATGGTGTGTCG GCGGCAGTATGATGCGCGTGTGGACCTCTGGTCTGTGGGGGTGATCCTGTACGAAGCCCTCTTTGGGCAG CCCCCCTTTGCCTCCAGATCGTTCTCAGAGCTAGAAGAAAAGATTCGCAGCAATCGGGTGATTGAGCTCC CTCTTCGGCCCCAACTCTCCCTAGACTGCCGGGACCTGTTGCAGCGACTTCTAGAGCGGGACCCCGCCCG TCGAATCTCCTTCAAGGACTTCTTTGCCCATCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGC CTGGCACAGGCAAGGGCCCTTGTGGTGGAGGCTGTGAAGAAGGACCAGGAGGGGGATGCTGCCGCTGCCC TGTCGCTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCGCTACACTACGAAGTGGATGCCCAGAGGAA GGAGGCAATTAAGGCGAAGGTGGGACAGTATGTGTCCCGGGCAGAGGAGCTCAAAGCCATTGTCTCCTCC TCCAATCAGGCCCTGCTAAGACAGGGCACAACTGTCCAAGAGCTGCTTCGAGAGATGGCCCGTGACAAAC CACGCCTCCTGGCTGCCCTGGAAGTGGCCTCAGCTGCCCTGGCCAAGGAGGAGGAAGCTGGCAAAGAGCA GGATGCCCTGGACCTGTACCAGCACAGCCTCGGGGAGCTGCTAGTGCTGTTGGCAGCAGAGGCCCCAGGC CGAAGGCGGGAGCTCCTTCACACCGAGGTTCAGAACCTCATGGCTCGAGCTGAATACCTGAAGGAGCAGA TCAAGATAAGGGAGTCTCACTGGGAAGCGGAGAGTCTGGACAAAGAGGGGCTGTCGGAGTCTGTTCGTAG TTCTTGCACACTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027895 |
| Insert Size | 1419 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027895.1, NP_082171.1 |
| RefSeq Size | 2826 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 71742 |
| UniProt ID | Q3U3Q1 |
| Gene Summary | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219200 | Ulk3 (tGFP-tagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3), (10ug) |
CNY 3,710.00 |
|
| MR219200 | Ulk3 (Myc-DDK-tagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3) |
CNY 3,330.00 |
|
| MR219200L3 | Lenti ORF clone of Ulk3 (Myc-DDK-tagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3) |
CNY 5,230.00 |
|
| MR219200L4 | Lenti ORF clone of Ulk3 (mGFP-tagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3) |
CNY 5,230.00 |
